公开(公告)号：US20040058389A1

公开(公告)日：2004-03-25

申请号：US10666412

申请日：2003-09-18

申请人： New Mexico State University Technology Transfer Corporation

发明人： Joseph Wang ,  Ronen Polsky ,  Kathryn L. Turner

IPC分类号： G01N033/53 ,  G01N033/553

CPC分类号： G01N33/54346 ,  C12Q1/6825 ,  G01N33/54326 ,  G01N33/553 ,  Y10S436/801 ,  Y10S436/805 ,  Y10S436/81 ,  Y10S436/815 ,  C12Q2537/125 ,  C12Q2563/116 ,  C12Q2563/149

摘要： Methods and devices for electrochemical detection of a specific binding pair member utilizing a microsphere with an incorporated electroactive marker, wherein a member of the specific binding pair to be detected is bound, directly or through one or more intermediates, to the microsphere. Multiple specific binding pair members may be detected by use of electrochemically distinguishable electroactive markers. Microspheres with incorporated electroactive markers may include one or more functional groups for binding members of specific binding pairs, and are preferably insoluble in aqueous solvents but soluble in selected organic solvents.

摘要翻译： 使用具有引入的电活性标记的微球体特异性结合对成员的电化学检测的方法和装置，其中要检测的特异性结合对的成员直接或通过一个或多个中间体结合到微球体。 可以通过使用电化学上可区分的电活性标记来检测多个特异性结合对成员。 具有引入的电活性标记的微球可以包括用于结合特异性结合对成员的一个或多个官能团，并且优选不溶于水性溶剂，但可溶于选定的有机溶剂。

公开(公告)号：US5872013A

公开(公告)日：1999-02-16

申请号：US539131

申请日：1995-10-04

申请人： Johannes Leonardus Maria Leunissen ,  Marc Joris De Brabander ,  Petrus Franciscus Elisabeth Maria Van de Plas

发明人： Johannes Leonardus Maria Leunissen ,  Marc Joris De Brabander ,  Petrus Franciscus Elisabeth Maria Van de Plas

IPC分类号： A61K39/00 ,  A61K39/395 ,  G01N33/543 ,  G01N33/553 ,  G01N33/58

CPC分类号： G01N33/54346 ,  G01N33/553 ,  G01N33/587 ,  Y10S436/801 ,  Y10S530/811

摘要： An aggregate for qualitatively or quantitatively determining bindable substances. Said aggregate comprises an ultra small colloidal particle and a specific binding agent characterized in that the mean diameter of the colloidal particle is below 2 nm. Preferably the size of the metal particle is selected so that the aggregate is capable of penetrating in standard biological specimens. The invention further provides a universally applicable detection system for determining bindable substances in particular in living material. Further there is provided a process for preparing said aggregate.

摘要翻译： 用于定性或定量测定可结合物质的总量。 所述骨料包含超小胶体颗粒和特异性粘合剂，其特征在于胶体颗粒的平均直径低于2nm。 优选选择金属颗粒的尺寸，使得聚集体能够在标准生物样品中渗透。 本发明还提供了一种普遍适用的检测系统，用于确定特别在生物材料中的可结合物质。 此外，提供了制备所述骨料的方法。

公开(公告)号：US5597910A

公开(公告)日：1997-01-28

申请号：US479817

申请日：1995-06-07

申请人： Satyanarayana R. Gudibande ,  John H. Kenten

发明人： Satyanarayana R. Gudibande ,  John H. Kenten

IPC分类号： G01N21/77 ,  C07F13/00 ,  C07F15/00 ,  C07H21/00 ,  C07H21/04 ,  C08G79/02 ,  C09K11/07 ,  C12Q1/68 ,  C12Q1/70 ,  G01N27/447 ,  C09K11/06 ,  G01T1/20

CPC分类号： C07H21/00 ,  C12Q1/6816 ,  C12Q1/6876 ,  C12Q1/703 ,  C12Q1/708 ,  G01N27/44726 ,  Y10S428/917 ,  Y10S436/80 ,  Y10S436/801 ,  Y10S436/805 ,  Y10S436/905 ,  Y10T436/143333

摘要： This invention relates to a new electrochemiluminescent (ECL) label for oligonucleotides using phosphoramidite chemistry.

摘要翻译： 本发明涉及使用亚磷酰胺化学的寡核苷酸的新的电化学发光（ECL）标记。

公开(公告)号：US5516697A

公开(公告)日：1996-05-14

申请号：US512950

申请日：1995-08-09

申请人： Marian L. Kruzel

发明人： Marian L. Kruzel

IPC分类号： G01N33/487 ,  G01N33/84 ,  G01N33/20

CPC分类号： G01N33/84 ,  Y10S436/801 ,  Y10S436/91

摘要： A simple, cost effective assay of iron is accomplished by using lactoferrin as part of a biosensor to detect iron in a sample. The lactoferrin releases protons when the iron is sequestered by the lactoferrin and the change in potential caused by the release of protons is measured by a potentiometer or pH sensing device. The sensing devices include the ion-selective or ion sensitive field effect transistors (ISFET), a potentiometric device or the pH indicator papers.

摘要翻译： 通过使用乳铁蛋白作为生物传感器的一部分来检测样品中的铁来实现铁的简单，成本有效的测定。 当铁由乳铁蛋白隔离时，乳铁蛋白释放质子，并且通过电位计或pH感测装置测量由质子释放引起的电位变化。 感测装置包括离子选择性或离子敏感场效应晶体管（ISFET），电位器件或pH指示纸。

公开(公告)号：US5445972A

公开(公告)日：1995-08-29

申请号：US95888

申请日：1993-07-22

申请人： Peter J. Tarcha ,  Thomas E. Rohr ,  Therese Cotton ,  Gary E. Winter

发明人： Peter J. Tarcha ,  Thomas E. Rohr ,  Therese Cotton ,  Gary E. Winter

IPC分类号： G01N21/65 ,  G01N33/543 ,  G01N33/532 ,  G01N33/533

CPC分类号： G01N33/54373 ,  G01N21/658 ,  G01N2021/653 ,  Y10S435/968 ,  Y10S436/80 ,  Y10S436/801 ,  Y10S436/805 ,  Y10T436/24

摘要： A Raman label which, upon radiation, produces a detectable Raman scattering signal in a ligand-binding assay for an analyte in a test sample. A conjugate containing a Raman label attached to a specific binding member.

摘要翻译： 在测试样品中的分析物的配体结合测定中，辐射后产生可检测的拉曼散射信号的拉曼标签。 含有附着于特定结合成员的拉曼标签的缀合物。

公开(公告)号：US5120643A

公开(公告)日：1992-06-09

申请号：US72459

申请日：1987-07-13

申请人： Shanfun Ching ,  Patricia Billing ,  Julian Gordon

发明人： Shanfun Ching ,  Patricia Billing ,  Julian Gordon

IPC分类号： G01N30/88 ,  G01N30/90 ,  G01N33/532 ,  G01N33/543 ,  G01N33/558 ,  G01N33/58 ,  G01N33/76 ,  G01N37/00

CPC分类号： G01N33/587 ,  G01N33/54366 ,  G01N33/54386 ,  G01N33/54393 ,  G01N33/558 ,  G01N33/585 ,  G01N33/76 ,  G01N30/90 ,  Y10S435/967 ,  Y10S435/97 ,  Y10S435/975 ,  Y10S436/801 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/826 ,  Y10S514/97

摘要： The present invention relates to improved specific binding assay methods, kits and devices utilizing chromatographically mobile specific binding reagents labelled with colloidal particles. Specific binding reagents labelled with colloidal particles such as gold and selenium may be subjected to rapid chromatographic solvent transport on chromatographic media by means of selected solvents and chromatographic transport facilitating agents. Further, impregnation of solid substrate materials with labile protein materials including colloidal particle and enzyme labelled reagents in the presence of meta-soluble proteins provides for the rapid resolubilization of such materials which have been dried onto such substrate materials.

公开(公告)号：US4454231A

公开(公告)日：1984-06-12

申请号：US212806

申请日：1980-12-04

申请人： Michael Cais ,  Moshe Shimoni

发明人： Michael Cais ,  Moshe Shimoni

IPC分类号： B01D11/04 ,  B01J8/00 ,  B01J8/34 ,  G01N25/14 ,  G01N33/53 ,  G01N33/537 ,  G01N33/58 ,  B65D71/00 ,  G01N35/00

CPC分类号： B01D11/04 ,  B01J8/003 ,  B01J8/34 ,  G01N33/537 ,  B01L3/5021 ,  Y10S435/975 ,  Y10S436/80 ,  Y10S436/801 ,  Y10S436/804 ,  Y10S436/808 ,  Y10S436/81 ,  Y10S436/811 ,  Y10S436/816 ,  Y10S436/817

摘要： The present invention relates to a new technique and device for mass transfer of one or more components from one liquid phase to another liquid phase, involving a physical separation of said two phases.According to the new technique, the mass transfer and physical separation are carried out in the same device. The device consists of a mixing-reservoir into which is fitted snugly a mixer-separator, having a channel in the vertical axis of the mixer-separator. The two substantially immiscible liquid solutions are introduced into the mixing reservoir, the phases are thoroughly mixed, by moving the mixer-separator in and out the mixing reservoir. After the spontaneous separation into an upper and lower phase, the upper phase is removed by pushing in the mixer-separator said upper phase being accumulated in a collecting container.The new technique and device can be successfully utilized in the general field of liquid-liquid extraction and particularly applied in immunoassays, where the two basic operations of mass transfer and physical separation of fractions are required.

摘要翻译： 本发明涉及用于将一种或多种组分从一个液相传质到另一个液相的新技术和装置，其涉及所述两相的物理分离。 根据新技术，传质和物理分离在相同的装置中进行。 该装置由混合容器组成，其中紧密地安装有混合器分离器，在混合器分离器的垂直轴上具有通道。 将两个基本上不混溶的液体溶液引入混合容器中，通过将混合器分离器移入和移出混合容器而将相充分混合。 在自发分离成上，下相之后，通过将混合器分离器中的上部相积聚在收集容器中来推动上相。 新技术和设备可以在液 - 液萃取的一般领域得到成功应用，特别适用于免疫测定，其中需要传质和物理分离两种基本操作。

公开(公告)号：US4230664A

公开(公告)日：1980-10-28

申请号：US5754

申请日：1979-01-23

申请人： Michael Cais

发明人： Michael Cais

IPC分类号： B01L3/14 ,  G01N33/532 ,  G01N33/537 ,  G01N33/16

CPC分类号： B01L3/5082 ,  G01N33/532 ,  G01N33/537 ,  Y10S436/801 ,  Y10S436/808 ,  Y10S436/81

摘要： A test pack kit for assaying for a ligand in a liquid medium. The kit comprises specially designed assay tubes, each tube having a cylindrical side arm which can be interconnected with the side arm of another assay tube. In addition, the pack kit contains antiserum; a conjugate of a metal labelling substance with the ligand; assay calibration standards--in vials containing known concentrations of unlabelled ligand; a separation agent in vials containing material used for separating the bound antibody--metal labelling complex from the unbound metal labelling substance and instrument calibration standards--in vials containing several known concentrations of the metal--labelling substance. Errors caused by pipetting or transferring of reagents from one container to another are practically eliminated.

摘要翻译： 用于测定液体介质中配体的测试包试剂盒。 该试剂盒包括特别设计的测定管，每个管具有可与另一测定管的侧臂互连的圆柱形侧臂。 此外，包装盒含有抗血清; 金属标记物质与配体的共轭物; 测定校准标准品 - 含有已知浓度的未标记配体的小瓶; 在包含用于从未结合的金属标记物质和仪器校准标准物分离结合的抗体 - 金属标记复合物的材料的小瓶中的分离剂 - 在含有几种已知浓度的金属标记物质的小瓶中。 实际上消除了将试剂从一个容器移液或转移到另一个容器造成的错误。

公开(公告)号：US4225575A

公开(公告)日：1980-09-30

申请号：US905552

申请日：1978-05-15

申请人： Roger N. Piasio ,  David A. Perry ,  Pangal N. Nayak

发明人： Roger N. Piasio ,  David A. Perry ,  Pangal N. Nayak

IPC分类号： C12Q1/00 ,  G01N33/50 ,  G01N33/543 ,  G01N33/16 ,  A61K43/00 ,  B01J1/22

CPC分类号： C12Q1/00 ,  G01N33/50 ,  G01N33/54366 ,  Y10S436/801 ,  Y10S436/804 ,  Y10S436/81

摘要： A method and apparatus for conducting chemical reactions at a liquid-solid interface wherein a reaction component is fixed on the surface of a solid phase immersed in a liquid phase containing a freely diffusing, mobile reaction component. The apparatus comprises a substantially smooth-surfaced matrix of appropriate geometrical configuration designed to provide a large surface to volume ratio, a short transfer distance from the mobile component to the fixed component distributed on the solid phase surface and to drain freely upon removal from the reaction liquid.

摘要翻译： 一种用于在液 - 固界面处进行化学反应的方法和装置，其中将反应组分固定在浸入含有自由扩散的可移动反应组分的液相中的固相的表面上。 该装置包括适当的几何构型的基本光滑表面的矩阵，其被设计成提供大的表面与体积比，从移动部件到分布在固相表面上的固定部件的短传输距离，并且在从反应中移除时自由排水 液体。

公开(公告)号：US4067959A

公开(公告)日：1978-01-10

申请号：US684775

申请日：1976-05-10

申请人： Gunner Bolz

发明人： Gunner Bolz

IPC分类号： G01N33/543 ,  G01N33/58 ,  G01N33/00 ,  A61K43/00

CPC分类号： G01N33/54306 ,  G01N33/58 ,  Y10S435/975 ,  Y10S436/80 ,  Y10S436/801 ,  Y10S436/804 ,  Y10S436/807

摘要： An indirect method for the quantitation of an antigen or antibody in a liquid sample. In the antigen assay, antigen of the same type as the sample antigen is adsorbed onto a solid support surface. Then, specific labelled antibody is immunologically reacted in solution with the sample antigen and the adsorbed antigen. The quantity of reacted labelled antibody on the solid support surface is then determined, as for example, fluorogen label by a fluorometer. The procedure is employed for detection of antibody by reversing the antigen and antibody components in each instance.

摘要翻译： 用于定量液体样品中抗原或抗体的间接方法。 在抗原测定中，与样品抗原相同类型的抗原被吸附在固体支持物表面上。 然后，特异性标记的抗体与溶液中的样品抗原和吸附的抗原进行免疫反应。 然后测定固体支持体表面上反应的标记抗体的量，例如通过荧光计的荧光标记。 该方法用于通过在每种情况下逆转抗原和抗体组分来检测抗体。