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    Soluble rubella E1 envelope protein variants
    1.
    发明授权
    Soluble rubella E1 envelope protein variants 有权
    可溶性风疹E1包膜蛋白变体

    公开(公告)号：US07604935B2

    公开(公告)日：2009-10-20

    申请号：US11584887

    申请日：2006-10-23

    申请人： Barbara Upmeier ,  Ralf Bollhagen ,  Alfred Engel ,  Elke Faatz ,  Peter Schaarschmidt ,  Christian Scholz ,  Toralf Zarnt

    发明人： Barbara Upmeier ,  Ralf Bollhagen ,  Alfred Engel ,  Elke Faatz ,  Peter Schaarschmidt ,  Christian Scholz ,  Toralf Zarnt

    IPC分类号： C12Q1/70 ,  C12N7/01 ,  A61K39/20

    CPC分类号： C07K14/005 ,  C07K2319/35 ,  C12N2770/36222 ,  Y10S530/826

    摘要： A soluble rubella E1 antigen variant is disclosed that comprises amino acids 334-409 of the native rubella E1 peptide, but lacks the C-terminal end and at least the transmembrane region and the anchor segment as well as at least the amino acids 143 to 164. Also described is a recombinant DNA molecule encoding the rubella E1 antigen variants which are recombinantly expressed as a chaperone fusion protein, refolded into a soluble and immunoreactive conformation, and further used for the serological detection of anti-rubella antibodies. In addition, also disclosed is a method for the detection, determination and quantification of anti-rubella antibodies of IgG and/or IgM subclass in a sample wherein the rubella E1 antigen is used as a capture reagent and/or binding partner for the antibodies.

    摘要翻译： 公开了可溶性风疹E1抗原变体，其包含天然风疹E1肽的氨基酸334-409，但缺少C末端和至少跨膜区和锚定区以及至少氨基酸143至164 还描述了编码风疹E1抗原变体的重组DNA分子，其被重组表达为伴侣融合蛋白，重折叠成可溶性和免疫反应性构象，并进一步用于抗风疹抗体的血清学检测。 此外，还公开了用于检测，测定和定量样品中IgG和/或IgM亚类的抗风疹抗体的方法，其中所述风疹E1抗原用作抗体的捕获试剂和/或结合配偶体。

    Soluble rubella E1 envelope protein variants
    3.
    发明申请
    Soluble rubella E1 envelope protein variants 有权
    可溶性风疹E1包膜蛋白变体

    公开(公告)号：US20070154883A1

    公开(公告)日：2007-07-05

    申请号：US11584887

    申请日：2006-10-23

    申请人： Barbara Upmeier ,  Ralf Bollhagen ,  Alfred Engel ,  Elke Faatz ,  Peter Schaarschmidt ,  Christian Scholz ,  Toralf Zarnt

    发明人： Barbara Upmeier ,  Ralf Bollhagen ,  Alfred Engel ,  Elke Faatz ,  Peter Schaarschmidt ,  Christian Scholz ,  Toralf Zarnt

    IPC分类号： C12Q1/70

    CPC分类号： C07K14/005 ,  C07K2319/35 ,  C12N2770/36222 ,  Y10S530/826

    摘要： The invention disclosed relates to a soluble rubella E1 antigen and variants of this peptide characterized by lacking at the C-terminal end at least the transmembrane region and the anchor segment as well as at least the amino acids 143 to 164 and containing at least the region spanning the disulfide bridges Cys 349-Cys 352 and Cys 368-401 whereas the N-terminus (Cys 349) of this region contains additionally at least 15 amino acids and/or the C-terminus (Cys 401) of this region contains additionally at least 8 amino acids of the adjacent rubella E1 antigen sequence. Also described are a recombinant DNA molecule encoding the rubella E1 antigen and variants which are recombinantly expressed as a chaperone fusion protein, refolded into a soluble and immunoreactive conformation, and further used for the serological detection of anti-rubella antibodies. In addition, also disclosed is a method for the detection, determination and quantification of anti-rubella antibodies of IgG and/or IgM subclass in a sample wherein the rubella E1 antigen is used as a capture reagent and/or binding partner for the antibodies.

    摘要翻译： 所公开的本发明涉及可溶性风疹E1抗原和该肽的变体，其特征在于至少在C-末端缺少至少跨膜区和锚段以及至少氨基酸143至164并且至少含有该区域 跨越二硫键Cys 349-Cys 352和Cys 368-401，而该区域的N末端（Cys 349）另外包含至少15个氨基酸和/或该区域的C末端（Cys 401） 相邻风疹E1抗原序列的至少8个氨基酸。 还描述了编码风疹E1抗原的重组DNA分子和重组表达为伴侣融合蛋白的变体，重新折叠成可溶性和免疫反应性构象，并进一步用于抗风疹抗体的血清学检测。 此外，还公开了用于检测，测定和定量样品中IgG和/或IgM亚类的抗风疹抗体的方法，其中所述风疹E1抗原用作抗体的捕获试剂和/或结合配偶体。

    Recombinant microbial 3-hydroxybutyrate dehydrogenase a process for its production and the use thereof
    10.
    发明授权
    Recombinant microbial 3-hydroxybutyrate dehydrogenase a process for its production and the use thereof 失效
    重组微生物3-羟基丁酸脱氢酶一种生产方法及其应用

    公开(公告)号：US06255093B1

    公开(公告)日：2001-07-03

    申请号：US09287097

    申请日：1999-04-07

    申请人： Rainer Schmuck ,  Rainer Müller ,  Harald Weisser ,  Alfred Engel ,  Kerstin Krüger

    发明人： Rainer Schmuck ,  Rainer Müller ,  Harald Weisser ,  Alfred Engel ,  Kerstin Krüger

    IPC分类号： C12N904

    CPC分类号： C12N9/0006

    摘要： DNA molecule comprising a structural gene which codes for a bacterial protein with the enzymatic activity of 3-hydroxybutyrate dehydrogenase (3-HBDH), a recombinant DNA containing the DNA molecule, microorganisms transformed accordingly, a process for obtaining 3-HBDH by culturing a suitable transformed microorganism as well as a method for the determination of ketone bodies in the presence of the enzyme. A structural gene from Rhodobacter sphaeroides is preferred.

    摘要翻译： 包含编码具有3-羟基丁酸脱氢酶（3-HBDH）的酶活性的细菌蛋白质的结构基因的DNA分子，含有该DNA分子的重组DNA，相应地转化的微生物，通过培养合适的方法获得3-HBDH的方法 转化的微生物以及在酶存在下测定酮体的方法。 来自球形红细菌的结构基因是优选的。