-
公开(公告)号：US08426167B2
公开(公告)日：2013-04-23
申请号：US11786847
申请日：2007-04-13
申请人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Urban Schmitt , Ariuna Bazarsuren , Peter Schaarschmidt
发明人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Urban Schmitt , Ariuna Bazarsuren , Peter Schaarschmidt
IPC分类号： C12P21/04
CPC分类号： G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20
摘要： The present invention relates to the cloning and expression of foreign protein or polypeptides in bacteria, such as Escherichia coli. In particular, this invention relates to expression tools comprising a FKBP-type peptidyl prolyl isomerase selected from the group consisting of FkpA, SlyD, and trigger factor; methods of recombinant protein expression, the recombinant polypeptides thus obtained, as well as to the use of such polypeptides.
-
2.发明申请Methods for producing fusion polypeptides or enhancing expression of fusion polypeptides 有权用于产生融合多肽或增强融合多肽表达的方法公开(公告)号：US20090028893A1
公开(公告)日：2009-01-29
申请号：US11786847
申请日：2007-04-13
申请人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Urban Schmitt , Ariuna Bazarsuren , Peter Schaarschmidt
发明人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Urban Schmitt , Ariuna Bazarsuren , Peter Schaarschmidt
CPC分类号： G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20
摘要： The present invention relates to the cloning and expression of foreign protein or polypeptides in bacteria, such as Escherichia coli. In particular, this invention relates to expression tools comprising a FKBP-type peptidyl prolyl isomerase selected from the group consisting of FkpA, SlyD, and trigger factor; methods of recombinant protein expression, the recombinant polypeptides thus obtained, as well as to the use of such polypeptides.
摘要翻译： 本发明涉及在细菌如大肠杆菌中克隆和表达外源蛋白质或多肽。 特别地，本发明涉及包含选自FkpA，SlyD和触发因子的FKBP型肽基脯氨酰异构酶的表达工具; 重组蛋白表达的方法，如此获得的重组多肽，以及这些多肽的用途。
-
3.发明授权Fusion polypeptides, vaccines and compositions of FKBP chaperones and target polypeptides 有权融合多肽，疫苗和FKBP伴侣和靶多肽的组合物公开(公告)号：US07244819B2
公开(公告)日：2007-07-17
申请号：US10179038
申请日：2002-06-24
申请人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Urban Schmitt , Ariuna Bazarsuren , Peter Schaarschmidt
发明人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Urban Schmitt , Ariuna Bazarsuren , Peter Schaarschmidt
IPC分类号： C01K1/00
CPC分类号： G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20
摘要： The present invention relates to the cloning and expression of foreign protein or polypeptides in bacteria, such as Escherichia coli. In particular, this invention relates to expression tools comprising a FKBP-type peptidyl prolyl isomerase selected from the group consisting of FkpA, SlyD, and trigger factor; methods of recombinant protein expression, the recombinant polypeptides thus obtained, as well as to the use of such polypeptides.
摘要翻译： 本发明涉及在细菌如大肠杆菌中克隆和表达外源蛋白质或多肽。 特别地，本发明涉及包含选自FkpA，SlyD和触发因子的FKBP型肽基脯氨酰异构酶的表达工具; 重组蛋白表达的方法，如此获得的重组多肽，以及这些多肽的用途。
-
公开(公告)号：US07244575B2
公开(公告)日：2007-07-17
申请号：US11088599
申请日：2005-03-24
申请人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Dorothea Sizmann
发明人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Dorothea Sizmann
CPC分类号： G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20 , G01N2800/2821
摘要： The present invention relates to the diagnosis of HIV infections. It especially teaches the production of a soluble retroviral surface glycoprotein-(or transmembrane glycoprotein)-chaperone complex and the advantageous use of a chaperone-antigen complex especially in the detection of antibodies to HIV in immunoassays, preferably according to the double antigen bridge concept, or as an immunogen. The invention also discloses soluble complexes comprising a variant of HIV-1 gp41 or a variant of HIV-2 gp36, respectively, and a chaperone selected from the peptidyl-prolyl-isomerase class of chaperones. Variants comprising specific amino-acid substitutions in the N-helical domain of HIV-1 gp41 or of HIV-2 gp36, respectively, are also described.
摘要翻译： 本发明涉及HIV感染的诊断。 它特别教导了可溶性逆转录病毒表面糖蛋白（或跨膜糖蛋白） - 辅助复合物的产生，并且优选地根据双抗原桥概念，优选使用伴侣 - 抗原复合物，特别是在免疫测定中检测HIV抗体中， 或作为免疫原。 本发明还公开了分别包含HIV-1 gp41的变体或HIV-2 gp36的变体的可溶性复合物，以及选自分子伴侣的肽基 - 脯氨酰基异构酶类的伴侣。 还描述了分别在HIV-1 gp41或HIV-2 gp36的N-螺旋结构域中包含特异性氨基酸取代的变体。
-
5.发明授权Soluble complexes of target proteins and peptidyl prolyl isomerase chaperones and methods of making and using them 有权靶蛋白和肽基脯氨酰异构酶伴侣的可溶性复合物及其制备和使用方法公开(公告)号：US06962982B2
公开(公告)日：2005-11-08
申请号：US10179905
申请日：2002-06-24
申请人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Dorothea Sizmann
发明人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Dorothea Sizmann
IPC分类号： A61K39/00 , A61K47/48 , C07K14/16 , C12N9/90 , G01N33/543 , G01N33/569 , A61K38/24 , C07K14/00
CPC分类号： G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20 , G01N2800/2821
摘要： The present invention relates to the diagnosis of HIV infections. It especially teaches the production of a soluble retroviral surface glycoprotein- (or transmembrane glycoprotein)-chaperone complex and the advantageous use of a chaperone-antigen complex especially in the detection of antibodies to HIV in immunoassays, preferably according to the double antigen bridge concept, or as an immunogen. The invention also discloses soluble complexes comprising a variant of HIV-1 gp41 or a variant of HIV-2 gp36, respectively, and a chaperone selected from the peptidyl-prolyl-isomerase class of chaperones. Variants comprising specific amino-acid substitutions in the N-helical domain of HIV-1 gp41 or of HIV-2 gp36, respectively, are also described.
摘要翻译： 本发明涉及HIV感染的诊断。 特别是教导了可溶性逆转录病毒表面糖蛋白（或跨膜糖蛋白） - 辅助复合物的产生，并且优选地根据双抗原桥概念，优选使用伴侣 - 抗原复合物，特别是在免疫测定中检测HIV抗体中， 或作为免疫原。 本发明还公开了分别包含HIV-1 gp41的变体或HIV-2 gp36的变体的可溶性复合物，以及选自分子伴侣的肽基 - 脯氨酰基异构酶类的伴侣。 还描述了分别在HIV-1 gp41或HIV-2 gp36的N-螺旋结构域中包含特异性氨基酸取代的变体。
-
公开(公告)号：US20050181358A1
公开(公告)日：2005-08-18
申请号：US11088599
申请日：2005-03-24
申请人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Dorothea Sizmann
发明人： Christian Scholz , Herbert Andres , Elke Faatz , Alfred Engel , Dorothea Sizmann
IPC分类号： A61K39/00 , A61K47/48 , C07K14/16 , C12N9/90 , G01N33/543 , G01N33/569 , C12Q1/70 , C12P21/06
CPC分类号： G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20 , G01N2800/2821
摘要： The present invention relates to the diagnosis of HIV infections. It especially teaches the production of a soluble retroviral surface glycoprotein-(or transmembrane glycoprotein)-chaperone complex and the advantageous use of a chaperone-antigen complex especially in the detection of antibodies to HIV in immunoassays, preferably according to the double antigen bridge concept, or as an immunogen. The invention also discloses soluble complexes comprising a variant of HIV-1 gp41 or a variant of HIV-2 gp36, respectively, and a chaperone selected from the peptidyl-prolyl-isomerase class of chaperones. Variants comprising specific amino-acid substitutions in the N-helical domain of HIV-1 gp41 or of HIV-2 gp36, respectively, are also described.
摘要翻译： 本发明涉及HIV感染的诊断。 特别是教导了可溶性逆转录病毒表面糖蛋白（或跨膜糖蛋白） - 辅助复合物的产生，并且优选地根据双抗原桥概念，优选使用伴侣 - 抗原复合物，特别是在免疫测定中检测HIV抗体中， 或作为免疫原。 本发明还公开了分别包含HIV-1 gp41的变体或HIV-2 gp36的变体的可溶性复合物，以及选自分子伴侣的肽基 - 脯氨酰基异构酶类的伴侣。 还描述了分别在HIV-1 gp41或HIV-2 gp36的N-螺旋结构域中包含特异性氨基酸取代的变体。
-
公开(公告)号：US09266962B2
公开(公告)日：2016-02-23
申请号：US14112108
申请日：2012-05-04
申请人： Herbert Andres , David Casagolda Vallribera , Hartmut Duefel , Michael Gerg , Christian Scholz , Michael Schraeml
发明人： Herbert Andres , David Casagolda Vallribera , Hartmut Duefel , Michael Gerg , Christian Scholz , Michael Schraeml
CPC分类号： C07K16/22 , C07K14/475 , C07K14/65 , C07K16/1203 , C07K16/40 , C07K2317/34 , C07K2317/565 , C07K2317/92 , C07K2319/00 , C07K2319/21 , C07K2319/22 , C07K2319/23 , C07K2319/24 , C07K2319/35 , C07K2319/41 , C07K2319/43 , C12N9/90 , C12Y502/01008 , G01N33/573 , G01N33/74 , G01N2333/99
摘要： Herein is reported a fusion polypeptide according to formula (I): NH2—S2—X1—S1—COOH, wherein X1 comprises either a random amino acid sequence or an amino acid sequence derived from a first polypeptide, S2 and S1 are non-overlapping amino acid sequences derived from a second polypeptide, and denotes a peptide bond, wherein the second polypeptide is a polypeptide with peptidyl-prolyl cis/trans-isomerase activity (PPIase activity) or is derived from the FKBP-fold domain family, wherein X1 is inserted in place of the insert-in-flap-domain of the second polypeptide.
摘要翻译： 本文报道了根据式（I）的融合多肽：NH2-S2-X1-S1-COOH，其中X1包含随机氨基酸序列或衍生自第一多肽的氨基酸序列，S2和S1不重叠 衍生自第二多肽的氨基酸序列，并且表示肽键，其中第二多肽是具有肽基 - 脯氨酰顺/反异构酶活性（PPIase活性）或衍生自FKBP-折叠域家族的多肽，其中X1是 插入第二多肽的插入片段结构域。
-
公开(公告)号：US20140178393A1
公开(公告)日：2014-06-26
申请号：US14112108
申请日：2012-05-04
申请人： Herbert Andres , David Casagolda Vallribera , Hartmut Duefel , Michael Gerg , Christian Scholz , Michael Schraeml
发明人： Herbert Andres , David Casagolda Vallribera , Hartmut Duefel , Michael Gerg , Christian Scholz , Michael Schraeml
IPC分类号： C12N9/90 , G01N33/573 , C07K16/40
CPC分类号： C07K16/22 , C07K14/475 , C07K14/65 , C07K16/1203 , C07K16/40 , C07K2317/34 , C07K2317/565 , C07K2317/92 , C07K2319/00 , C07K2319/21 , C07K2319/22 , C07K2319/23 , C07K2319/24 , C07K2319/35 , C07K2319/41 , C07K2319/43 , C12N9/90 , C12Y502/01008 , G01N33/573 , G01N33/74 , G01N2333/99
摘要： Herein is reported a fusion polypeptide according to formula (I): NH2—S2—X1—S1—COOH, wherein X1 comprises either a random amino acid sequence or an amino acid sequence derived from a first polypeptide, S2 and S1 are non-overlapping amino acid sequences derived from a second polypeptide, and denotes a peptide bond, wherein the second polypeptide is a polypeptide with peptidyl-prolyl cis/trans-isomerase activity (PPIase activity) or is derived from the FKBP-fold domain family, wherein X1 is inserted in place of the insert-in-flap-domain of the second polypeptide.
摘要翻译： 本文报道了根据式（I）的融合多肽：NH2-S2-X1-S1-COOH，其中X1包含随机氨基酸序列或衍生自第一多肽的氨基酸序列，S2和S1不重叠 衍生自第二多肽的氨基酸序列，并且表示肽键，其中第二多肽是具有肽基 - 脯氨酰顺/反异构酶活性（PPIase活性）或衍生自FKBP-折叠域家族的多肽，其中X1是 插入第二多肽的插入片段结构域。
-
公开(公告)号：US07731938B2
公开(公告)日：2010-06-08
申请号：US11764913
申请日：2007-06-19
IPC分类号： A61K49/00
CPC分类号： G01N33/57419 , G01N2333/4742
摘要： The present invention relates to the assessment of colorectal cancer. It discloses the use of the CYFRA 21-1 assay in the assessment of colorectal cancer. It also relates to a method for assessing colorectal cancer in vitro using a liquid sample, derived from an individual by measuring CYFRA 21-1 in said sample. Measurement of CYFRA 21-1 can, e.g., be used in the early detection or in the follow-up of patients with colorectal cancer.
摘要翻译： 本发明涉及结肠直肠癌的评估。 它公开了CYFRA 21-1测定在结肠直肠癌评估中的应用。 它还涉及一种通过在所述样品中测量CYFRA 21-1从体外衍生的液体样品体外评估结肠直肠癌的方法。 CYFRA 21-1的测量可以例如用于早期检测或结直肠癌患者的随访。
-
公开(公告)号：US20090155820A1
公开(公告)日：2009-06-18
申请号：US11820769
申请日：2007-06-20
IPC分类号： G01N33/574 , C12Q1/34 , C12Q1/48
CPC分类号： G01N33/57419
摘要： The present invention relates to the diagnosis of colorectal cancer. It discloses the use of protein ASC (apoptosis-associated speck-like protein containing a caspase-associated recruitment domain) in the diagnosis of colorectal cancer. It relates to a method for diagnosis of colorectal cancer from a liquid sample, derived from an individual by measuring ASC in said sample. Measurement of ASC can, e.g., be used in the early detection or diagnosis of colorectal cancer.
摘要翻译： 本发明涉及结肠直肠癌的诊断。 它公开了在结直肠癌诊断中使用蛋白质ASC（含凋亡相关的斑点状蛋白质，含有胱天蛋白酶相关募集域）。 它涉及一种通过测量所述样品中的ASC从个体衍生的来自液体样品的结肠直肠癌的诊断方法。 ASC的测量可以例如用于结肠直肠癌的早期检测或诊断。
-
-
-
-
-
-
-
-
-
搜索结果
29 条记录 (耗时 0.031 秒)