公开(公告)号：US07138254B2

公开(公告)日：2006-11-21

申请号：US10361481

申请日：2003-02-07

申请人： Stevan Bogdan Jovanovich ,  Oscar Salas-Solano ,  Jeng-Thun Li

发明人： Stevan Bogdan Jovanovich ,  Oscar Salas-Solano ,  Jeng-Thun Li

IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C07H21/00

CPC分类号： C12Q1/6806 ,  B01J2219/00286 ,  B01J2219/00369 ,  B01J2219/00511 ,  B01J2219/00585 ,  B01J2219/00691 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01L3/0241 ,  B01L3/5025 ,  B01L7/52 ,  B01L2300/0838 ,  B01L2300/1844 ,  B01L2400/0409 ,  C12Q1/6869 ,  C40B40/06 ,  C40B60/14 ,  C12Q2565/518 ,  C12Q2535/101 ,  C12Q2527/125

摘要： Methods for preparing nanoscale reactions using nucleic acids or proteins are presented. Nucleic acids are captured saturably, yet reversibly, on the internal surface of the reaction chamber, typically a capillary. Excess nucleic acid is removed and the reaction is performed directly within the capillary. Proteins are captured specifically and saturably on the modified inner surface of the reaction chamber, typically a capillary. Excess protein is removed and the reaction is performed directly within the capillary. Devices for effecting the methods of the invention and a system designed advantageously to utilize the methods for high throughput reactions involving nucleic acids or proteins are also provided.

公开(公告)号：US06476118B1

公开(公告)日：2002-11-05

申请号：US09507496

申请日：2000-02-18

申请人： Barry L. Karger ,  Lev Kotler ,  Wolfgang Goetzinger ,  Haihong Zhou ,  Zoran Sosic ,  Oscar Salas-Solano

发明人： Barry L. Karger ,  Lev Kotler ,  Wolfgang Goetzinger ,  Haihong Zhou ,  Zoran Sosic ,  Oscar Salas-Solano

IPC分类号： C08L3104

CPC分类号： G01N27/44747 ,  C08L33/24 ,  C08L33/26 ,  C08L2205/02 ,  C08L2666/04 ,  C08L33/00

摘要： A method of separating a mixture of biopolymers of various lengths using a blend of two linear hydrophilic polymers, particularly polyacrylamides (LPA) or substituted polyacrylamides, one with high weight-average molecular mass and another with low weight-average molecular mass, each present at a low concentration but wherein the concentration of the high weight-average molecular mass LPA is above its entanglement threshold, is disclosed. The matrix is excellent for use in methods of nucleic acid analysis, particularly long read length DNA sequencing.

摘要翻译： 使用两种直链亲水性聚合物，特别是聚丙烯酰胺（LPA）或取代的聚丙烯酰胺（一种具有高重均分子量，另一种具有低重均分子量）的共混物的混合物分离各种长度的生物聚合物的混合物的方法， 公开了低浓度，但其中高重均分子量LPA的浓度高于其缠结阈值。 该基质非常适用于核酸分析方法，特别是长读长度DNA测序。