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    Antimicrobial peptides from bovine neutrophis
    3.
    发明授权
    Antimicrobial peptides from bovine neutrophis 失效
    来自牛嗜中性粒细胞的抗菌肽

    公开(公告)号：US06211148B1

    公开(公告)日：2001-04-03

    申请号：US08988705

    申请日：1997-12-11

    申请人： Michael E. Selsted ,  James S. Cullor

    发明人： Michael E. Selsted ,  James S. Cullor

    IPC分类号： A61K3816

    CPC分类号： C07K14/4723 ,  A61K38/00

    摘要： The present invention provides a new family of cysteine-rich antimicrobial peptides isolated from bovine neutrophils herein named beta defensins. Thirteen structurally homologous peptides were purified to homogeneity from a granule-rich cytoplasmic fraction of purified blood neutrophils. These antimicrobial compounds are useful in human and veterinary medicine, and as agents in agricultural, food science, and industrial applications.

    摘要翻译： 本发明提供了一种从牛中性粒细胞分离的命名为β-防御素的新的富含半胱氨酸的抗微生物肽家族。 将13个结构同源的肽从纯化的血液中性粒细胞的富颗粒细胞质部分纯化至均质。 这些抗菌化合物可用于人类和兽医学，也可用于农业，食品科学和工业应用。

    Broad spectrum antimicrobial compounds and methods of use
    4.
    发明授权
    Broad spectrum antimicrobial compounds and methods of use 失效
    广谱抗菌化合物及其使用方法

    公开(公告)号：US5324716A

    公开(公告)日：1994-06-28

    申请号：US715271

    申请日：1991-06-14

    申请人： Michael E. Selsted ,  James S. Cullor

    发明人： Michael E. Selsted ,  James S. Cullor

    IPC分类号： A61K38/00 ,  A61P31/04 ,  C07K7/08 ,  A61K37/00 ,  A61K37/02 ,  C07K5/00 ,  C07K7/00

    CPC分类号： C07K7/08 ,  A61K38/00

    摘要： The invention provides a broad spectrum antimicrobial compound that includes a tryptophan-rich peptide exhibiting antimicrobial activity. A method of microbicidal inhibition or microbistatic inhibition of microbial growth is also provided. The method includes administering to an environment capable of sustaining microbial growth a microbicidally or microbistatically effective amount of a tryptophan-rich peptide exhibiting antimicrobial activity.

    摘要翻译： 本发明提供了一种广谱抗微生物化合物，其包括显示抗菌活性的富含色氨酸的肽。 还提供了杀微生物抑制或微生物抑制微生物生长的方法。 该方法包括向能够维持微生物生长的环境施用具有抗微生物活性的微生物或微生物有效量的富含色氨酸的肽。

    Antimicrobial peptides from bovine neutrophils
    5.
    发明授权
    Antimicrobial peptides from bovine neutrophils 失效
    来自牛嗜中性粒细胞的抗菌肽

    公开(公告)号：US5821224A

    公开(公告)日：1998-10-13

    申请号：US356832

    申请日：1994-12-13

    申请人： Michael E. Selsted ,  James S. Cullor

    发明人： Michael E. Selsted ,  James S. Cullor

    IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K39/395 ,  A61P31/04 ,  C07K14/47 ,  C07K16/18 ,  C12N5/02 ,  C12P21/02 ,  C12P21/08 ,  A61K38/17 ,  C12N15/12

    CPC分类号： C07K14/4723 ,  A61K38/00

    摘要： The present invention provides a new family of cysteine-rich antimicrobial peptides isolated from bovine neutrophils herein named beta defensins. Thirteen structurally homologous peptides were purified to homogeneity from a granule-rich cytoplasmic fraction of purified blood neutrophils. These antimicrobial compounds are useful in human and veterinary medicine, and as agents in agricultural, food science, and industrial applications.

    摘要翻译： 本发明提供了一种从牛中性粒细胞分离的命名为β-防御素的新的富含半胱氨酸的抗微生物肽家族。 将13个结构同源的肽从纯化的血液中性粒细胞的富颗粒细胞质部分纯化至均质。 这些抗菌化合物可用于人类和兽医学，也可用于农业，食品科学和工业应用。

    Method and apparatus for monitoring body parts of animals
    7.
    发明授权
    Method and apparatus for monitoring body parts of animals 失效
    用于监测动物身体部位的方法和装置

    公开(公告)号：US4491126A

    公开(公告)日：1985-01-01

    申请号：US395830

    申请日：1982-01-06

    申请人： James S. Cullor

    发明人： James S. Cullor

    IPC分类号： A61D1/00 ,  A61D7/00 ,  A61M1/00 ,  C12N5/16 ,  A61B19/00 ,  A61M25/00

    CPC分类号： A61M39/0247 ,  A61D7/00 ,  C12N5/163 ,  C12N2502/02 ,  C12N2510/02

    摘要： An improved method and apparatus is disclosed for adding fluids to, or removing fluids from, a body part or organ of an animal which minimizes animal trauma and permits rapid, easy, repeated fluid or low viscosity gels transfers. The apparatus includes a valve assembly having a tubular fluid-conveying element adapted for fixed connection to the animal, along with a fluid conduit connected between the valve element and a specific internal body part or organ, such as a cow's uterus. In use, a syringe is employed to introduce or remove fluids from the body part, through the valve assembly and connected conduit. The invention is especially adapted for introducing, recovering cell lines producing monoclonal antibodies or other biologically active products in large mammals, and facilitates monitoring of antibody production as well as administration of nutrients to enhance cell line growth.

    摘要翻译： 公开了一种改进的方法和装置，用于向动物的身体部位或器官添加流体或从其中去除流体，其使动物创伤最小化并允许快速，容易，重复的流体或低粘度凝胶转移。 该装置包括具有管状流体输送元件的阀组件，其适于与动物的固定连接，以及连接在阀元件和特定内部身体部分或器官（例如母牛的子宫）之间的流体导管。 在使用中，使用注射器通过阀组件和连接的导管从体部引入或去除流体。 本发明特别适用于引入，回收在大型哺乳动物中产生单克隆抗体或其他生物活性产物的细胞系，并且有助于监测抗体产生以及营养物质的施用以增强细胞系生长。

    Composition and method for in vitro cell culture
    8.
    发明授权
    Composition and method for in vitro cell culture 失效
    体外细胞培养的组成和方法

    公开(公告)号：US4987079A

    公开(公告)日：1991-01-22

    申请号：US390453

    申请日：1989-08-04

    申请人： James S. Cullor

    发明人： James S. Cullor

    IPC分类号： A61D7/00 ,  A61M1/00 ,  C12N5/16

    CPC分类号： A61M39/0247 ,  A61D7/00 ,  C12N5/163 ,  C12N2502/02 ,  C12N2510/02

    摘要： An improved method and apparatus is disclosed for adding fluids to, or removing fluids from, a body part or organ of an animal which minimizes animal trauma and permits rapid, easy, repeated fluid or low viscosity gel transfers. The apparatus includes a tubular fluid-conveying element adapted for fixed connection to the animal, along with a flexible fluid conduit connected between the element and a specific internal body part or organ, such as a cow's uterus. In use, a syringe is employed to introduce or remove fluids from the body part, through the tubular element and connected conduit. The invention is especially adapted for introducing and recovering cell lines producing monoclonal antibodies or other biologically active products in large mammals, and fascilitates monitoring of antibody production as well as administration of nutrients to enhance cell line growth. In preferred monoclonal antibody production procedures, the cells are initially educated through in vitro contact with ambient fluid of the selected organ (e.g., allantoic fluid from a pregnant host and fetal calf serum), whereupon the educated cells are inoculated and allowed to incubate in the host. It has been found particularly advantageous to employ the allantois of a pregnant mammal for antibody production, and some or all of the cell incubation period therein should occur after the onset of immunocompetency of the fetus.

    摘要翻译： 公开了一种改进的方法和装置，用于向动物的身体部位或器官添加流体或从其中去除流体，其使动物创伤最小化并允许快速，容易，重复的流体或低粘度凝胶转移。 该装置包括适于与动物的固定连接的管状流体输送元件，以及连接在元件与特定内部身体部位或器官（例如母牛的子宫）之间的柔性流体导管。 在使用中，使用注射器通过管状元件和连接的管道从体部引入或移除流体。 本发明特别适用于引入和回收在大型哺乳动物中产生单克隆抗体或其他生物活性产物的细胞系，并且监测抗体产生以及施用营养物以增强细胞系生长。 在优选的单克隆抗体制备方法中，通过体外接触所选器官的环境流体（例如，来自怀孕宿主的尿囊液和胎牛血清）来初步培养细胞，接受接受培养的细胞并允许其在 主办。 已经发现特别有利的是，使用怀孕哺乳动物的尿囊来进行抗体产生，并且其中的部分或全部细胞孵育期应在胎儿免疫能力发生之后发生。