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8 条记录 (耗时 0.021 秒)

    System and method for introduction and stabilization of genes in Thermus sp.
    6.
    发明授权
    System and method for introduction and stabilization of genes in Thermus sp. 失效
    引入和稳定栖热菌属基因的系统和方法

    公开(公告)号：US06861258B2

    公开(公告)日：2005-03-01

    申请号：US10085388

    申请日：2002-02-28

    申请人： Kevin J. Kayser ,  Ho-Shin Park ,  John J. Kilbane, II

    发明人： Kevin J. Kayser ,  Ho-Shin Park ,  John J. Kilbane, II

    IPC分类号： C12N1/21 ,  C12N15/74 ,  C12Q1/68 ,  C12P21/00

    CPC分类号： C12R1/20 ,  C12N15/74 ,  C12Q1/6897

    摘要： A method for introducing and stabilizing heterologous and recombinant genes in a thermophilic host in which a characteristic gene defining a detectable host characteristic is inactivated or deleted from the thermophilic host, resulting in a modified thermophilic host expressing an absence of the detectable host characteristic. A DNA fragment of interest is inserted into the modified thermophilic host together with an intact characteristic gene, whereby the detectable host characteristic is restored to the thermophilic host, thereby enabling detection and confirmation of successful transformation using plasmid vectors and integration of the DNA fragment into the chromosome of the thermophilic host.

    摘要翻译： 一种用于在嗜热宿主中引入和稳定异源和重组基因的方法，其中定义可检测的宿主特征的特征基因从嗜热宿主中失活或缺失，导致表达不存在可检测宿主特征的经修饰的嗜热宿主。 将感兴趣的DNA片段与完整特征基因一起插入修饰的嗜热性宿主中，由此将可检测的宿主特征恢复到嗜热宿主，从而使得能够使用质粒载体检测和确认成功的转化并将DNA片段整合到 嗜热宿主的染色体。

    Method for metabolizing carbazole in petroleum
    8.
    发明授权
    Method for metabolizing carbazole in petroleum 失效
    在石油中代谢咔唑的方法

    公开(公告)号：US06943006B2

    公开(公告)日：2005-09-13

    申请号：US10658691

    申请日：2003-09-09

    申请人： Kevin J. Kayser ,  John J. Kilbane, II

    发明人： Kevin J. Kayser ,  John J. Kilbane, II

    IPC分类号： C10G32/00 ,  C12N15/52 ,  C12P7/22 ,  C12P13/00 ,  C12N1/20 ,  C12N1/21 ,  C12N9/02 ,  C12P7/02

    CPC分类号： C12P13/001 ,  C10G32/00 ,  C12N15/52 ,  C12P7/22

    摘要： A method for selective cleavage of C—N bonds genes that encode for at least one enzyme suitable for conversion of carbazole to 2-aminobiphenyl-2,3-diol are combined with a gene encoding an amidase suitable for selectively cleaving a C—N bond in 2-aminobiphenyl-2,3-diol, forming an operon that encodes for cleavage of both C—N bonds of said carbazole. The operon is inserted into a host culture which, in turn, is contacted with the carbazole, resulting in selective cleavage of both C—N bonds of the carbazole. Also disclosed is a new microorganism that expresses a carbazole degradation trait constitutively and a method for degrading carbazole employing this microorganism.

    摘要翻译： 用于选择性切割CN键的方法将编码至少一种适用于将咔唑转化为2-氨基联苯-2,3-二醇的酶的基因与编码适合于选择性裂解2-氨基联苯中的CN键的酰胺酶的基因组合 -2,3-二醇，形成编码所述咔唑的两个CN键切割的操纵子。 将操纵子插入宿主培养物中，其继而与咔唑接触，导致咔唑的两个C-N键的选择性裂解。 还公开了组成型表达咔唑降解性状的新微生物和使用该微生物降解咔唑的方法。