公开(公告)号：US20200325510A1

公开(公告)日：2020-10-15

申请号：US16913761

申请日：2020-06-26

申请人： Codexis, Inc.

发明人： Haibin Chen ,  Yong Koy Bong ,  Fabien L. Cabirol ,  Anupam Gohel Prafulchandra ,  Tao Li ,  Jeffrey C. Moore ,  Martina Quintanar-Audelo ,  Yang Hong ,  Steven J. Collier ,  Derek Smith

IPC分类号： C12P17/12 ,  C12P17/18 ,  C12N9/02 ,  C12P13/24 ,  C12P13/04

摘要： The present disclosure provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.

公开(公告)号：US10787689B2

公开(公告)日：2020-09-29

申请号：US16655547

申请日：2019-10-17

申请人： CODEXIS, INC.

发明人： Haibin Chen ,  Steven J. Collier ,  Jovana Nazor ,  Joly Sukumaran ,  Derek Smith ,  Jeffrey C. Moore ,  Gregory Hughes ,  Jacob Janey ,  Gjalt W. Huisman ,  Scott J. Novick ,  Nicholas J. Agard ,  Oscar Alvizo ,  Gregory A. Cope ,  Wan Lin Yeo ,  Stefanie Ng Minor

IPC分类号： C12N9/06 ,  C12P13/02 ,  C12P13/00 ,  C12P17/18 ,  C12P13/04 ,  C12P13/06 ,  C12P17/10 ,  C12P17/12 ,  C12P17/16

摘要： The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

公开(公告)号：US10308966B2

公开(公告)日：2019-06-04

申请号：US16195480

申请日：2018-11-19

申请人： CODEXIS, INC.

发明人： Haibin Chen ,  Steven J. Collier ,  Jovana Nazor ,  Joly Sukumaran ,  Derek Smith ,  Jeffrey C. Moore ,  Gregory Hughes ,  Jacob Janey ,  Gjalt W. Huisman ,  Scott J. Novick ,  Nicholas J. Agard ,  Oscar Alvizo ,  Gregory A. Cope ,  Wan Lin Yeo ,  Stefanie Ng Minor

IPC分类号： C12N9/06 ,  C12P13/00 ,  C12P13/02 ,  C12P13/04 ,  C12P13/06 ,  C12P17/10 ,  C12P17/12 ,  C12P17/16 ,  C12P17/18

摘要： The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

公开(公告)号：US20180187221A1

公开(公告)日：2018-07-05

申请号：US15899834

申请日：2018-02-20

申请人： CODEXIS, INC.

发明人： Haibin Chen ,  Steven J. Collier ,  Jovana Nazor ,  Joly Sukumaran ,  Derek Smith ,  Jeffrey C. Moore ,  Gregory Hughes ,  Jacob Janey ,  Gjalt W. Huisman ,  Scott J. Novick ,  Nicholas J. Agard ,  Oscar Alvizo ,  Gregory A. Cope ,  Wan Lin Yeo ,  Stefanie Ng Minor

IPC分类号： C12P13/02 ,  C12N9/06 ,  C12P13/06 ,  C12P17/18 ,  C12P13/00 ,  C12P17/16 ,  C12P17/10 ,  C12P17/12 ,  C12P13/04

CPC分类号： C12P13/02 ,  C12N9/0028 ,  C12P13/001 ,  C12P13/04 ,  C12P13/06 ,  C12P17/10 ,  C12P17/12 ,  C12P17/165 ,  C12P17/185 ,  C12P17/188 ,  C12Y105/01 ,  C12Y105/01023 ,  C12Y105/01024 ,  C12Y105/01028 ,  Y02P20/52

摘要： The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

公开(公告)号：US09963685B2

公开(公告)日：2018-05-08

申请号：US15443792

申请日：2017-02-27

申请人： Codexis, Inc.

发明人： Martina Quintanar-Audelo ,  Ellen Eberhard ,  Jovana Nazor ,  Derek Smith ,  Cuixia Wang

IPC分类号： C12P13/00 ,  C12P17/18 ,  C12N9/10 ,  C07D487/04 ,  C12P17/08

CPC分类号： C12N9/1096 ,  C07D487/04 ,  C12P13/001 ,  C12P17/182 ,  C12Y206/01

摘要： The present disclosure provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The disclosure also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral amine compounds.

公开(公告)号：US20180066292A1

公开(公告)日：2018-03-08

申请号：US15792446

申请日：2017-10-24

申请人： CODEXIS, INC.

发明人： Haibin Chen ,  Steven J. Collier ,  Jovana Nazor ,  Joly Sukumaran ,  Derek Smith ,  Jeffrey C. Moore ,  Gregory Hughes ,  Jacob Janey ,  Gjalt W. Huisman ,  Scott J. Novick ,  Nicholas J. Agard ,  Oscar Alvizo ,  Gregory A. Cope ,  Wan Lin Yeo ,  Stefanie Ng Minor

IPC分类号： C12P13/02 ,  C12N9/06 ,  C12P17/18 ,  C12P17/12 ,  C12P17/10 ,  C12P17/16 ,  C12P13/00 ,  C12P13/06 ,  C12P13/04

CPC分类号： C12P13/02 ,  C12N9/0028 ,  C12P13/001 ,  C12P13/04 ,  C12P13/06 ,  C12P17/10 ,  C12P17/12 ,  C12P17/165 ,  C12P17/185 ,  C12P17/188 ,  C12Y105/01 ,  C12Y105/01023 ,  C12Y105/01024 ,  C12Y105/01028 ,  Y02P20/52

摘要： The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

公开(公告)号：US09803224B2

公开(公告)日：2017-10-31

申请号：US15605061

申请日：2017-05-25

申请人： CODEXIS, INC.

发明人： Haibin Chen ,  Steven J. Collier ,  Jovana Nazor ,  Joly Sukumaran ,  Derek Smith ,  Jeffrey C. Moore ,  Gregory Hughes ,  Jacob Janey ,  Gjalt W. Huisman ,  Scott J. Novick ,  Nicholas J. Agard ,  Oscar Alvizo ,  Gregory A. Cope ,  Wan Lin Yeo ,  Stefanie Ng Minor

IPC分类号： C12N9/06 ,  C12P13/02 ,  C12P13/04 ,  C12P17/18 ,  C12P17/10 ,  C12P13/06 ,  C12P17/12 ,  C12P17/16 ,  C12P13/00

CPC分类号： C12P13/02 ,  C12N9/0028 ,  C12P13/001 ,  C12P13/04 ,  C12P13/06 ,  C12P17/10 ,  C12P17/12 ,  C12P17/165 ,  C12P17/185 ,  C12P17/188 ,  C12Y105/01 ,  C12Y105/01023 ,  C12Y105/01024 ,  C12Y105/01028 ,  Y02P20/52

摘要： The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

公开(公告)号：US20170022527A1

公开(公告)日：2017-01-26

申请号：US15286900

申请日：2016-10-06

申请人： CODEXIS, INC.

发明人： Haibin Chen ,  Steven J. Collier ,  Jovana Nazor ,  Joly Sukumaran ,  Derek Smith ,  Jeffrey C. Moore ,  Gregory Hughes ,  Jacob Janey ,  Gjalt W. Huisman ,  Scott J. Novick ,  Nicholas J. Agard ,  Oscar Alvizo ,  Gregory A. Cope ,  Wan Lin Yeo ,  Stefanie Ng Minor

IPC分类号： C12P13/02 ,  C12P17/18 ,  C12P17/12 ,  C12P17/16 ,  C12N9/06 ,  C12P13/00

CPC分类号： C12P13/02 ,  C12N9/0028 ,  C12P13/001 ,  C12P13/04 ,  C12P13/06 ,  C12P17/10 ,  C12P17/12 ,  C12P17/165 ,  C12P17/185 ,  C12P17/188 ,  C12Y105/01 ,  C12Y105/01023 ,  C12Y105/01024 ,  C12Y105/01028 ,  Y02P20/52

摘要： The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

摘要翻译： 本公开提供了具有亚胺还原酶活性的工程改造的多肽，编码工程改造的亚胺还原酶的多核苷酸，能够表达工程化亚胺还原酶的宿主细胞，以及使用这些工程改造的多肽与一系列酮和胺底物化合物以制备仲胺和叔胺的方法 产品化合物。

公开(公告)号：US09476077B2

公开(公告)日：2016-10-25

申请号：US14636988

申请日：2015-03-03

申请人： Codexis, Inc.

发明人： Ryan Fong ,  Xiyun Zhang ,  Chris Noriega ,  Nicholas Agard ,  Anupam Gohel ,  Derek Smith

IPC分类号： C12N9/24 ,  C12N1/21 ,  C12N15/56 ,  C12P19/14 ,  C12P7/10 ,  C12P19/02 ,  C12P7/14 ,  C12P7/16

CPC分类号： C12P19/14 ,  C12N9/2402 ,  C12N9/248 ,  C12N9/2482 ,  C12P7/14 ,  C12P7/16 ,  C12P19/02 ,  C12Y302/01008 ,  C12Y302/01037 ,  Y02E50/16

摘要： The present invention provides fungal xylanase and/or beta-xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase and/or beta-xylosidases, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.

公开(公告)号：US20160002688A1

公开(公告)日：2016-01-07

申请号：US14768408

申请日：2014-02-24

申请人： CODEXIS, INC.

发明人： Martina Quintanar-Audelo ,  Ellen Eberhard ,  Jovana Nazor ,  Derek Smith ,  Cuixia Wang

IPC分类号： C12P17/18 ,  C12P13/00 ,  C12N9/10

CPC分类号： C12N9/1096 ,  C07D487/04 ,  C12P13/001 ,  C12P17/182 ,  C12Y206/01

摘要： The present disclosure provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The disclosure also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral amine compounds.

摘要翻译： 本公开提供了可用于在工业上相关条件下合成手性胺化合物的工程化转氨酶多肽。 本公开还提供编码工程改造的转氨酶多肽的多核苷酸，能够表达工程化转氨酶的宿主细胞，以及使用工程转氨酶来生产手性胺化合物的方法。