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公开(公告)号：US20080113401A1
公开(公告)日：2008-05-15
申请号：US11742173
申请日：2007-04-30
申请人： Colin Cooper , Jay Lewington , Edman Tsang , Robbie Burch
发明人： Colin Cooper , Jay Lewington , Edman Tsang , Robbie Burch
IPC分类号： C12Q1/02
CPC分类号： G01N33/52 , C12Q1/02 , C12Q1/66 , C12Q2304/60 , G01N2430/10 , Y10S435/909
摘要： Assays for detecting potentially toxicdanalytes utilize a preparation of eukaryotic or prokaryotic cells which have signal-generating metabolic activity. The sensitivity of such assays is significantly improved by the employment of a particulate solid-phase adsorbent e.g. silica gel, which can be preliminarily mixed either with the suspected toxic analyte or with the cellular reagent. It is found that the presence of either the adsorbent-analyte combination or the adsorbent-cellular reagent combination results in an amplification of the decrease of the intensity of the generated signal, thereby improving the detection capability of the assay, particularly with respect to low concentrations of toxicants.
摘要翻译： 用于检测潜在毒性分析物的测定法利用具有信号产生代谢活性的真核细胞或原核细胞的制备物。 通过使用颗粒状固相吸附剂，可以显着改善这种测定的灵敏度。 硅胶，其可以预先与疑似有毒分析物或细胞试剂混合。 发现吸附剂 - 分析物组合或吸附剂 - 细胞试剂组合的存在导致产生的信号强度的降低的扩增，从而提高了测定的检测能力，特别是在低浓度下 的毒物。
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公开(公告)号：US07214505B1
公开(公告)日：2007-05-08
申请号：US09979699
申请日：2000-05-05
申请人： Colin Cooper , Edman Tsang , Robbie Burch , Jay Lewington
发明人： Colin Cooper , Edman Tsang , Robbie Burch , Jay Lewington
IPC分类号： C12Q1/02
CPC分类号： G01N33/52 , C12Q1/02 , C12Q1/66 , C12Q2304/60 , G01N2430/10 , Y10S435/909
摘要： Cell-based reagents and methods of using the reagents for detecting analytes are provided. The adsorbing of cells with signal-generating metabolic activity to solid supports has been found to improve the sensitivity of known cell based assays. Signal-generating cells adsorbed to a solid support are introduced to a test agent, and the measured decrease in metabolic signal provides a measure of the toxicity of the test agent.
摘要翻译： 提供了基于细胞的试剂和使用试剂检测分析物的方法。 已经发现将具有产生信号的代谢活性的细胞吸附到固体支持物上可以提高已知基于细胞的测定的灵敏度。 吸附到固体支持物上的信号发生细胞被引入到测试试剂中，并且所测量的代谢信号的降低提供了测试试剂的毒性的量度。
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公开(公告)号：US20100192706A1
公开(公告)日：2010-08-05
申请号：US12449855
申请日：2008-02-28
申请人： Michael Roy Fairs , Thomas David Ford , Piers Sebastian Harding , Gary Stephen Howard , Barry Boyes , John Walter Czajka , Douglas Jason Green , Jay Lewington , Carmelo Volpe , Colin Fewster , Jason Betley , Catherine Mills , William Richard Mawer
发明人： Michael Roy Fairs , Thomas David Ford , Piers Sebastian Harding , Gary Stephen Howard , Barry Boyes , John Walter Czajka , Douglas Jason Green , Jay Lewington , Carmelo Volpe , Colin Fewster , Jason Betley , Catherine Mills , William Richard Mawer
IPC分类号： G01N1/10
CPC分类号： G01N1/28 , B01L3/502 , B01L3/5082 , B01L2300/046 , B01L2300/0681 , B01L2300/087 , B01L2400/0683 , G01N2035/1086
摘要： Apparatus for preparing a biological sample for PCR analysis has an inlet (4) for receiving the sample (15) and a sieve (13) through which the sample is forced by screwing down an inlet cap (16). A lysis solution between two rupturable seals (11 and 12) is effective to disrupt the cells of the sample and release nucleic acid. The apparatus is mounted releasably on a PCR machine (2) having a motor (2′) releasably coupled with a drive mechanism (3, 36,40) in the apparatus to effect a rotary and a vertical up and down movement of its components (30, 31, 32, 42). The apparatus has a transparent cuvette (5) and a needle (71) extending to the lower end of the cuvette, by which the prepared sample is dispensed to the cuvette for PCR analysis.
摘要翻译： 用于制备用于PCR分析的生物样品的装置具有用于接收样品（15）的入口（4）和通过拧入入口盖（16）来迫使样品被迫的筛子。 两个可破裂的密封件（11和12）之间的裂解溶液有效地破坏样品的细胞并释放核酸。 该装置可释放地安装在具有可释放地与设备中的驱动机构（3,36,40）联接的马达（2'）的PCR机（2）上，以实现其部件的旋转和垂直上下运动 30，31，32，42）。 该装置具有透明比色皿（5）和延伸到比色皿下端的针（71），通过该针将准备的样品分配到比色皿进行PCR分析。
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公开(公告)号：US08327724B2
公开(公告)日：2012-12-11
申请号：US12449855
申请日：2008-02-28
申请人： Michael Roy Fairs , Thomas David Ford , Piers Sebastian Harding , Gary Stephen Howard , Barry Boyes , John Walter Czajka , Douglas Jason Green , Jay Lewington , Carmelo Volpe , Colin Fewster , Jason Betley , Catherine Mills , William Richard Mawer
发明人： Michael Roy Fairs , Thomas David Ford , Piers Sebastian Harding , Gary Stephen Howard , Barry Boyes , John Walter Czajka , Douglas Jason Green , Jay Lewington , Carmelo Volpe , Colin Fewster , Jason Betley , Catherine Mills , William Richard Mawer
IPC分类号： G01N1/00
CPC分类号： G01N1/28 , B01L3/502 , B01L3/5082 , B01L2300/046 , B01L2300/0681 , B01L2300/087 , B01L2400/0683 , G01N2035/1086
摘要： Apparatus for preparing a biological sample for PCR analysis has an inlet (4) for receiving the sample (15) and a sieve (13) through which the sample is forced by screwing down an inlet cap (16). A lysis solution between two rupturable seals (11 and 12) is effective to disrupt the cells of the sample and release nucleic acid. The apparatus is mounted releasably on a PCR machine (2) having a motor (2′) releasably coupled with a drive mechanism (3, 36,40) in the apparatus to effect a rotary and a vertical up and down movement of its components (30, 31, 32, 42). The apparatus has a transparent cuvette (5) and a needle (71) extending to the lower end of the cuvette, by which the prepared sample is dispensed to the cuvette for PCR analysis.
摘要翻译： 用于制备用于PCR分析的生物样品的装置具有用于接收样品（15）的入口（4）和通过拧入入口盖（16）来迫使样品被迫的筛子。 两个可破裂的密封件（11和12）之间的裂解溶液有效地破坏样品的细胞并释放核酸。 该装置可释放地安装在具有可释放地与设备中的驱动机构（3,36,40）联接的马达（2'）的PCR机（2）上，以实现其部件的旋转和垂直上下运动 30，31，32，42）。 该装置具有透明比色皿（5）和延伸到比色皿下端的针（71），通过该针将准备的样品分配到比色皿进行PCR分析。
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公开(公告)号：US20090066507A1
公开(公告)日：2009-03-12
申请号：US12216312
申请日：2008-07-02
IPC分类号： G08B21/00
CPC分类号： B01L7/52 , B01L2200/14 , B01L2200/147 , B01L2200/185 , B01L2300/023 , B01L2300/024 , G01N35/00871
摘要： A method and apparatus for providing assay information to a portable detection unit, by way of a remote server in communication with the portable detection unit or a consumable with stored information for use with the portable detection unit. Global Positioning System (GPS) information is provided to a portable detection unit having a GPS receiver, so that the unit can determine its current position, including altitude. Based on the determined position, assays and parameters, such as PCR melt temperatures, can be selected. Assays to be performed by the portable detection unit can be selected based on the results of a prior assay.
摘要翻译： 一种用于通过与便携式检测单元通信的远程服务器或具有用于便携式检测单元的存储信息的消费品向便携式检测单元提供测定信息的方法和装置。 全球定位系统（GPS）信息被提供给具有GPS接收器的便携式检测单元，使得该单元可以确定其当前位置，包括高度。 基于确定的位置，可以选择测定和参数，例如PCR熔融温度。 可以基于先前测定的结果来选择由便携式检测单元执行的测定。
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公开(公告)号：US20140141419A1
公开(公告)日：2014-05-22
申请号：US13979323
申请日：2012-01-13
申请人： Jay Lewington , Rohit Mistry , J. Aquilez Sanchez
发明人： Jay Lewington , Rohit Mistry , J. Aquilez Sanchez
CPC分类号： C12Q1/686 , C12Q1/6851 , G16B30/00 , C12Q2527/101 , C12Q2561/101 , C12Q2537/165 , C12Q2539/101
摘要： Provided herein are systems and methods for quantitatively monitoring target amplicons produced by polymerase chain reaction (PCR). In particular, quantitative monitoring of target amplicon(s) in a single-tube PCR reaction without separate calibration reactions are provided.
摘要翻译： 本文提供了用于定量监测由聚合酶链反应（PCR）产生的靶扩增子的系统和方法。 特别地，提供了单管PCR反应中靶扩增子的定量监测，而没有单独的校准反应。
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公开(公告)号：US08138909B2
公开(公告)日：2012-03-20
申请号：US12216312
申请日：2008-07-02
IPC分类号： G08B29/00
CPC分类号： B01L7/52 , B01L2200/14 , B01L2200/147 , B01L2200/185 , B01L2300/023 , B01L2300/024 , G01N35/00871
摘要： A method and apparatus for providing assay information to a portable detection unit, by way of a remote server in communication with the portable detection unit or a consumable with stored information for use with the portable detection unit. Global Positioning System (GPS) information is provided to a portable detection unit having a GPS receiver, so that the unit can determine its current position, including altitude. Based on the determined position, assays and parameters, such as PCR melt temperatures, can be selected. Assays to be performed by the portable detection unit can be selected based on the results of a prior assay.
摘要翻译： 一种用于通过与便携式检测单元通信的远程服务器或具有用于便携式检测单元的存储信息的消费品向便携式检测单元提供测定信息的方法和装置。 全球定位系统（GPS）信息被提供给具有GPS接收器的便携式检测单元，使得该单元可以确定其当前位置，包括高度。 基于确定的位置，可以选择测定和参数，例如PCR熔融温度。 可以基于先前测定的结果来选择由便携式检测单元执行的测定。
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公开(公告)号：US20100261179A1
公开(公告)日：2010-10-14
申请号：US12682123
申请日：2008-10-17
申请人： Jason Betley , Barry Boyes , John Walter Czajka , Michael Roy Fairs , Colin Fewster , Thomas David Ford , Douglas Jason Green , Piers Sebastian Harding , Gary Stephen Howard , Jay Lewington , William Richard Mawer , Catherine Mills , James Onslow , Carmelo Volpe
发明人： Jason Betley , Barry Boyes , John Walter Czajka , Michael Roy Fairs , Colin Fewster , Thomas David Ford , Douglas Jason Green , Piers Sebastian Harding , Gary Stephen Howard , Jay Lewington , William Richard Mawer , Catherine Mills , James Onslow , Carmelo Volpe
CPC分类号： B03C1/288 , B03C2201/18 , B03C2201/26 , G01N35/0098 , G01N2035/00366
摘要： The application provides sample preparation devices and analyses. The devices and analyzes allow for the rapid preparation and analysis of samples using a variety of techniques, including PCR, by even unskilled users.
摘要翻译： 该应用程序提供样品制备装置和分析。 这些设备和分析允许甚至不熟练的用户使用各种技术（包括PCR）快速准备和分析样品。
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9.发明授权Method of making bioluminescent assay reagent based on non-viable E. coli 有权基于不存在的大肠杆菌制备生物发光测定试剂的方法公开(公告)号：US06524810B2
公开(公告)日：2003-02-25
申请号：US09728320
申请日：2000-12-01
申请人： Jay Lewington , Katherine Isles , Sandy Primrose
发明人： Jay Lewington , Katherine Isles , Sandy Primrose
IPC分类号： G01N3352
CPC分类号： C12Q1/18 , G01N33/5014
摘要： A cell-derived assay reagent prepared from cells which have been killed by treatment with an antibiotic selected from the bleomycin-phleomycin family of antibiotics but which retain a signal-generating metabolic activity such as bioluminescence.
摘要翻译： 通过用选自博来霉素 - 腐草霉素抗生素家族但保留信号产生代谢活性例如生物发光的抗生素治疗已被杀死的细胞制备的细胞衍生测定试剂。
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