公开(公告)号：US20160130647A1

公开(公告)日：2016-05-12

申请号：US14921466

申请日：2015-10-23

Applicant: Complete Genomics, Inc.

Inventor： Snezana Drmanac ,  Handong Li ,  Radoje Drmanac ,  Eric Harness ,  Chongjun Xu

IPC: C12Q1/68 ,  C07H19/10 ,  C07H19/207

CPC classification number: C12Q1/6874 ,  C07H19/10 ,  C07H19/20 ,  C07H19/207 ,  C12Q1/6869 ,  C12Q2525/113 ,  C12Q2563/131

Abstract: Novel fluorescent nucleotide analogues are provided herein. Also provided herein are methods of using the nucleotide analogues in sequencing-by-synthesis and signal confinement methods.

Abstract translation: 本文提供了新的荧光核苷酸类似物。 本文还提供了在合成序列和信号限制方法中使用核苷酸类似物的方法。

公开(公告)号：US11485966B2

公开(公告)日：2022-11-01

申请号：US16754713

申请日：2017-10-11

Applicant: MGI TECH CO., LTD. ,  COMPLETE GENOMICS, INC.

Inventor： Hui Wang ,  Xun Xu ,  Jin Yang ,  Ao Chen ,  Chongjun Xu ,  Wenwei Zhang

IPC: C12N15/10 ,  C12N15/11 ,  C12Q1/68

Abstract: The present invention provides a method for improving the loading of nucleic acid on a solid support by contacting the solid support with a poloxamer-containing reagent. The present invention also provides a method for improving the stability of a nucleic acid on a solid support, comprising contacting a nucleic acid molecule with a partially double-strand oligonucleotide before or after loading the nucleic acid molecule on a solid support, so as to cause the nucleic acid molecule to hybridize with the oligonucleotide. The present invention also provides a combined use of the two methods.

公开(公告)号：US20230272467A1

公开(公告)日：2023-08-31

申请号：US18144121

申请日：2023-05-05

Applicant: MGI TECH CO., LTD. ,  COMPLETE GENOMICS, INC.

Inventor： Jin Yang ,  Xun Xu ,  Hui Wang ,  Bin Xie ,  Zhuokun Li ,  Shengming Zhao ,  Ao Chen ,  Chongjun Xu ,  Wenwei Zhang ,  Ming Ni

IPC: C12Q1/6869 ,  C12Q1/6876 ,  G01N21/64

CPC classification number: C12Q1/6869 ,  C12Q1/6876 ,  G01N21/6486 ,  B01L2200/10

Abstract: The present invention provides a method for sequencing a nucleic acid using an immersion reaction protocol. The immersion reaction protocol comprises sequentially immersing a solid support having nucleic acid molecules immobilized thereon in different reaction containers to realize nucleic acid sequencing.

公开(公告)号：US11692221B2

公开(公告)日：2023-07-04

申请号：US16635131

申请日：2017-08-01

Applicant: MGI TECH CO., LTD. ,  COMPLETE GENOMICS, INC.

Inventor： Jin Yang ,  Xun Xu ,  Hui Wang ,  Bin Xie ,  Zhuokun Li ,  Shengming Zhao ,  Ao Chen ,  Chongjun Xu ,  Wenwei Zhang ,  Ming Ni

IPC: C12Q1/6869 ,  C12Q1/6876 ,  G01N21/64

CPC classification number: C12Q1/6869 ,  C12Q1/6876 ,  G01N21/6486 ,  B01L2200/10

Abstract: The present invention provides a method for sequencing a nucleic acid using an immersion reaction protocol. The immersion reaction protocol comprises sequentially immersing a solid support having nucleic acid molecules immobilized thereon in different reaction containers to realize nucleic acid sequencing.

公开(公告)号：US10190162B2

公开(公告)日：2019-01-29

申请号：US14921466

申请日：2015-10-23

Applicant: Complete Genomics, Inc.

Inventor： Snezana Drmanac ,  Handong Li ,  Radoje Drmanac ,  Eric Harness ,  Chongjun Xu

IPC: C12Q1/68 ,  C12Q1/6874 ,  C07H19/207 ,  C07H19/10 ,  C07H19/20 ,  C12Q1/6869

Abstract: Novel fluorescent nucleotide analogs are provided herein. Also provided herein are methods of using the nucleotide analogs in sequencing-by-synthesis and signal confinement methods.