公开(公告)号：US11517879B2

公开(公告)日：2022-12-06

申请号：US17107600

申请日：2020-11-30

Applicant: Cytiva Bioprocess R&D AB

Inventor： Martin Hall ,  Sture Larsson ,  Andreas Muranyi ,  Gustav Rodrigo ,  Jinyu Zou ,  Per-Mikael Aberg

IPC: B01J20/24 ,  B01D15/38 ,  B01J20/286 ,  C07K1/22 ,  B01J20/32 ,  C07K14/31 ,  C07K16/00 ,  C07K17/00 ,  B01J20/28 ,  B01J20/285 ,  B01J20/289 ,  C07K17/10

Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIF) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.

公开(公告)号：US11124539B2

公开(公告)日：2021-09-21

申请号：US16348534

申请日：2017-11-14

Applicant: Cytiva BioProcess R&D AB

Inventor： Johan William Ohman ,  Mats Ander ,  Therese Graner ,  Anneli Jorsback ,  Jinyu Zou

IPC: C07K1/22 ,  B01J20/32 ,  B01D15/38 ,  B01J20/24 ,  B01J20/285 ,  B01J20/286

Abstract: The present invention relates to the field of chromatography and more specifically to producing protein affinity chromatography resins comprising affinity ligands based on a N-terminal fragment of a split intein, such as DnaE from Nostoc punctiforme, as well as methods for using the same. The N-terminal fragments are produced in inclusion bodies in bacterial cells.