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    TRICHODERMA REESEI HOST CELLS EXPRESSING A GLUCOAMYLASE FROM ASPERGILLUS FUMIGATUS AND METHODS OF USE THEREOF
    2.
    发明申请
    TRICHODERMA REESEI HOST CELLS EXPRESSING A GLUCOAMYLASE FROM ASPERGILLUS FUMIGATUS AND METHODS OF USE THEREOF 有权

    公开(公告)号：US20210047664A1

    公开(公告)日：2021-02-18

    申请号：US17002091

    申请日：2020-08-25

    申请人： DANISCO US INC

    发明人： JING GE ,  Ling Hua ,  Sung Ho Lee ,  Jalsen Li ,  Jayarama K. Shetty ,  Zhongmei Tang ,  Bo Zhang ,  Kun Zhong

    IPC分类号： C12P19/14 ,  C12N9/34 ,  C12P19/02

    摘要： Fungal glucoamylases from Aspergillus fumigatus—expressed in Trichoderma reesei host cells (AfGATR) are provided. Trichoderma reesei host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA Aspergillus fumigatus. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as Aspergillus kawachii alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by Aspergillus niger glucoamylase (AnGA) or native AfGA expressed in Aspergillus fumigatus. AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.

    TRICHODERMA REESEI HOST CELLS EXPRESSING A GLUCOAMYLASE FROM ASPERGILLUS FUMIGATUS AND METHODS OF USE THEREOF
    3.
    发明申请
    TRICHODERMA REESEI HOST CELLS EXPRESSING A GLUCOAMYLASE FROM ASPERGILLUS FUMIGATUS AND METHODS OF USE THEREOF 审中-公开
    表达来自青蒿素的谷氨酰胺酶的TRICHODERMA REESEI HOST细胞及其使用方法

    公开(公告)号：US20160115509A1

    公开(公告)日：2016-04-28

    申请号：US14649167

    申请日：2013-11-21

    申请人： DANISCO US INC.

    发明人： Jing Ge ,  Ling Hua ,  Sung Ho Lee ,  Jalsen Li ,  Jayarama K. Shetty ,  Zhongmei Tang ,  Bo Zhang ,  Kun Zhong

    IPC分类号： C12P19/14 ,  C12P19/02 ,  C12N9/34

    CPC分类号： C12P19/14 ,  C12N9/2428 ,  C12P19/02 ,  C12Y302/01003 ,  Y02E50/17

    摘要： Fungal glucoamylases from Aspergillus fumigatus—expressed in Trichoderma reesei host cells (AfGATR) are provided. Trichoderma reesei host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA Aspergillus fumigatus. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as Aspergillus kawachii alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by Aspergillus niger glucoamylase (AnGA) or native AfGA expressed in Aspergillus fumigatus. AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.

    摘要翻译： 提供了在里氏木霉宿主细胞（AfGATR）中表达的来自烟曲霉的真菌葡糖淀粉酶。 里氏木霉宿主细胞以更高或至少相当的水平将AfGATR表达为天然表达的AfGA烟曲霉（Aspergillus fumigatus）。 包括AfGA1TR和AfGA2TR的AfGATR在升高的温度和低pH下表现出高活性，因此在α-淀粉酶例如曲霉曲霉α-淀粉酶（AkAA）的存在下，可以有效地使用AfGATR。 与由烟曲霉中表达的黑曲霉葡糖淀粉酶（AnGA）或天然AfGA催化的糖化产物相比，AfGATR有利地将淀粉糖化催化剂显着富集DP1（即葡萄糖）的寡糖组合物。 AfGATR如AfGA1TR，AfGA2TR或其变体可以以比AnGA和天然表达的AfGA更低的剂量使用以产生相当水平的葡萄糖。