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    METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS CLAVATUS FOR SACCHARIFICATION
    5.
    发明申请
    METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS CLAVATUS FOR SACCHARIFICATION 有权
    使用来自青蒿素的ALPHA-AMYLASE进行分离的方法

    公开(公告)号：US20130323798A1

    公开(公告)日：2013-12-05

    申请号：US13888303

    申请日：2013-05-06

    Applicant: Danisco US Inc.

    Inventor： Jing Ge ,  Ling Hua ,  Martijn Silvan Scheffers ,  Zhongmei Tang ,  Marco Van Brussel-Zwijnen ,  Casper Vroemen ,  Bo Zhang ,  Kathleen A. Clarkson ,  Jacquelyn A. Huitink ,  Paula Johanna Maria Teunissen

    IPC: C12P19/14 ,  C12C7/04

    CPC classification number: C12P7/14 ,  C12C7/04 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/17

    Abstract: A fungal α-amylase is provided from Aspergillus clavatus (AcAmyl). AcAmyl has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

    Abstract translation: 从曲霉菌（AcAmyl）提供真菌α-淀粉酶。 AcAmyl具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为分批方法进行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmyl还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。

    Method of using α-amylase from Aspergillus clavatus for saccharification
    8.
    发明授权
    Method of using α-amylase from Aspergillus clavatus for saccharification 有权
    使用曲霉菌α-淀粉酶进行糖化的方法

    公开(公告)号：US09365871B2

    公开(公告)日：2016-06-14

    申请号：US14561085

    申请日：2014-12-04

    Applicant: DANISCO US INC.

    Inventor： Jing Ge ,  Ling Hua ,  Martijn Silvan Scheffers ,  Zhongmei Tang ,  Marco Van Brussel-Zwijnen ,  Casper Vroemen ,  Bo Zhang ,  Kathleen A. Clarkson ,  Jacquelyn A. Huitink ,  Paula Johanna Maria Teunissen

    IPC: C12P19/14 ,  C12C7/04 ,  C12P7/14

    CPC classification number: C12P7/14 ,  C12C7/04 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/17

    Abstract: A fungal α-amylase is provided from Aspergillus clavatus (AcAmy1). AcAmy1 has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

    Abstract translation: 真菌α-淀粉酶由曲霉菌（AcAmy1）提供。 AcAmy1具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为间歇法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmy1还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。

    TRICHODERMA REESEI HOST CELLS EXPRESSING A GLUCOAMYLASE FROM ASPERGILLUS FUMIGATUS AND METHODS OF USE THEREOF
    9.
    发明申请
    TRICHODERMA REESEI HOST CELLS EXPRESSING A GLUCOAMYLASE FROM ASPERGILLUS FUMIGATUS AND METHODS OF USE THEREOF 审中-公开
    表达来自青蒿素的谷氨酰胺酶的TRICHODERMA REESEI HOST细胞及其使用方法

    公开(公告)号：US20160115509A1

    公开(公告)日：2016-04-28

    申请号：US14649167

    申请日：2013-11-21

    Applicant: DANISCO US INC.

    Inventor： Jing Ge ,  Ling Hua ,  Sung Ho Lee ,  Jalsen Li ,  Jayarama K. Shetty ,  Zhongmei Tang ,  Bo Zhang ,  Kun Zhong

    IPC: C12P19/14 ,  C12P19/02 ,  C12N9/34

    CPC classification number: C12P19/14 ,  C12N9/2428 ,  C12P19/02 ,  C12Y302/01003 ,  Y02E50/17

    Abstract: Fungal glucoamylases from Aspergillus fumigatus—expressed in Trichoderma reesei host cells (AfGATR) are provided. Trichoderma reesei host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA Aspergillus fumigatus. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as Aspergillus kawachii alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by Aspergillus niger glucoamylase (AnGA) or native AfGA expressed in Aspergillus fumigatus. AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.

    Abstract translation: 提供了在里氏木霉宿主细胞（AfGATR）中表达的来自烟曲霉的真菌葡糖淀粉酶。 里氏木霉宿主细胞以更高或至少相当的水平将AfGATR表达为天然表达的AfGA烟曲霉（Aspergillus fumigatus）。 包括AfGA1TR和AfGA2TR的AfGATR在升高的温度和低pH下表现出高活性，因此在α-淀粉酶例如曲霉曲霉α-淀粉酶（AkAA）的存在下，可以有效地使用AfGATR。 与由烟曲霉中表达的黑曲霉葡糖淀粉酶（AnGA）或天然AfGA催化的糖化产物相比，AfGATR有利地将淀粉糖化催化剂显着富集DP1（即葡萄糖）的寡糖组合物。 AfGATR如AfGA1TR，AfGA2TR或其变体可以以比AnGA和天然表达的AfGA更低的剂量使用以产生相当水平的葡萄糖。

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