公开(公告)号：US20180208949A1

公开(公告)日：2018-07-26

申请号：US15934636

申请日：2018-03-23

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus NOORDAM ,  Michael Petrus Jozef BERKHOUT ,  Aida HISENI

IPC: C12P7/10 ,  C12P19/14 ,  C12P17/10 ,  C12P5/02 ,  C12P7/16 ,  C12P7/18 ,  C12P35/00 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P19/02 ,  C12P7/20

Abstract: The invention relates to a process for the preparation of a sugar product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; and d) optionally recovery of a sugar product; wherein during part of the time of the enzymatic hydrolysis, oxygen is added to the ligno-cellulosic material and during part of the time of the enzymatic hydrolysis less oxygen is added to the ligno-cellulosic material compared to the other part of the time of the enzymatic hydrolysis, preferably no oxygen is added to the ligno-cellulosic material.

公开(公告)号：US20200224223A1

公开(公告)日：2020-07-16

申请号：US16837637

申请日：2020-04-01

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus NOORDAM ,  Michael Petrus Jozef BERKHOUT ,  Aida HISENI

IPC: C12P7/10 ,  C12P19/14 ,  C12P19/02 ,  C12P5/02 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P17/10 ,  C12P35/00

Abstract: The invention relates to a process for the preparation of a sugar product from lignocellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; and d) optionally recovery of a sugar product; wherein during part of the time of the enzymatic hydrolysis, oxygen is added to the ligno-cellulosic material and during part of the time of the enzymatic hydrolysis less oxygen is added to the ligno-cellulosic material compared to the other part of the time of the enzymatic hydrolysis, preferably no oxygen is added to the ligno-cellulosic material.

公开(公告)号：US20160102300A1

公开(公告)日：2016-04-14

申请号：US14893186

申请日：2014-06-02

Applicant: DSM IP ASSETS B.V.

Inventor： Aida HISENI ,  Igor GALAEV ,  Luppo EDENS

IPC: C12N11/08 ,  C12C11/11 ,  C12C5/00 ,  C12N11/06 ,  C12N9/62

CPC classification number: C12N11/08 ,  C12C5/004 ,  C12C11/11 ,  C12N9/62 ,  C12N11/06 ,  C12Y304/21026

Abstract: The present invention relates to immobilized proline-specific endoprotease, wherein the proline-specific endoprotease is immobilized on a carrier comprising methacrylate which has been functionalized with amino dimethylene, and wherein the carrier has a particle size range of 100 to 400 μm. The invention also relates to a process for producing beer comprising the steps of preparing a mash, fermenting the beer, and stabilizing the beer, wherein the beer is incubated with the immobilized proline-specific endoprotease.

Abstract translation: 本发明涉及固定化的脯氨酸特异性内切蛋白酶，其中脯氨酸特异性内切蛋白酶固定在已经用氨基二亚甲基官能化的甲基丙烯酸酯的载体上，其中载体的粒度范围为100-400μm。 本发明还涉及一种生产啤酒的方法，包括制备糊状物，发酵啤酒和稳定啤酒的步骤，其中啤酒与固定化的脯氨酸特异性内切蛋白酶一起温育。