公开(公告)号：US20220064607A1

公开(公告)日：2022-03-03

申请号：US17419079

申请日：2019-12-30

Applicant: DSM IP Assets B.V.

Inventor： Peter Louis HOUSTON ,  René Marcel DE JONG ,  Valmik Kanubhai VYAS ,  René VERWAAL ,  Jenna McMAHON ,  Anna SYMBOR-NAGRABSKA

IPC: C12N9/10 ,  C12P7/62 ,  C12N1/18

Abstract: The present invention is related to production of retinyl acetate generated via enzymatic conversion of retinol, said process including the use of modified enzymes with improved activity.

公开(公告)号：US20240279706A1

公开(公告)日：2024-08-22

申请号：US18292227

申请日：2021-07-27

Applicant: DSM IP ASSETS B.V.

Inventor： Peter Louis HOUSTON ,  Valmik Kanubhai VYAS ,  Anna SYMBOR-NAGRABSKA ,  Reed Chadbourne DOTEN ,  Christopher Mark FARRELL ,  Ethan LAM

IPC: C12P23/00 ,  C12N1/16 ,  C12N9/10

CPC classification number: C12P23/00 ,  C12N1/16 ,  C12N9/1029

Abstract: The present invention is related to a novel process for production of retinyl acetate in a host cell, particularly oleaginous yeast such as e.g. Yarrowia, wherein the product purity could be increased with reduction of unwanted side-products. Particularly, the novel process comprises fermentation in the presence of ethanol, such as e.g. in a fed-batch fermentation process. Such process is especially useful in a biotechnological process for production of vitamin A.

公开(公告)号：US20230265443A1

公开(公告)日：2023-08-24

申请号：US18017819

申请日：2021-07-22

Applicant: DSM IP ASSETS B.V.

Inventor： Peter Louis HOUSTON ,  Valmik Kanubhai VYAS ,  René Marcel DE JONG ,  Anna SYMBOR-NAGRABSKA ,  Ethan LAM

IPC: C12N15/81 ,  C12N1/16 ,  C12P23/00 ,  C12N9/20

CPC classification number: C12N15/815 ,  C12N1/16 ,  C12P23/00 ,  C12N9/20 ,  C12N2500/36 ,  C12Y301/01003

Abstract: The present invention is related to a novel process for production of retinyl acetate in a host cell, particularly oleaginous yeast such as e.g. Yarrowia, growing on triglyceride oils, such as e.g. vegetable oil, wherein the host cell exhibits modified lipase activity in such a way that conversion of retinol into fatty acid retinyl esters (FAREs) is reduced or abolished. Such process is especially useful in a biotechnological process for production of vitamin A.