公开(公告)号：US20200277644A1

公开(公告)日：2020-09-03

申请号：US16649771

申请日：2018-09-25

Applicant: DSM IP ASSETS B.V.

Inventor： Nathalie BALCH ,  Paul BLOMQUIST ,  Reed DOTEN ,  Peter HOUSTON ,  Ethan LAM ,  Jenna MCMAHON ,  Joshua TRUEHEART ,  Celine VIAROUGE ,  René Marcel DE JONG

IPC: C12P23/00 ,  C12N9/02

Abstract: The present invention is related to a novel enzymatic process for production of vitamin A aldehyde (retinal) via stereoselective conversion of beta-carotene which process includes the use of trans-selective enzymes having activity as beta-carotene oxidases (BCOs), in particular having preference for trans-retinal. 5 Said process is in particular useful for biotechnological production of vitamin A.

公开(公告)号：US20240218336A1

公开(公告)日：2024-07-04

申请号：US18439987

申请日：2024-02-13

Applicant: DSM IP ASSETS B.V.

Inventor： Nathalie BALCH ,  Paul BLOMQUIST ,  Reed DOTEN ,  Peter HOUSTON ,  Ethan LAM ,  Jenna MCMAHON ,  Joshua TRUEHEART ,  Celine VIAROUGE

IPC: C12N9/04 ,  C12P23/00

CPC classification number: C12N9/0006 ,  C12P23/00 ,  C12Y101/01105

Abstract: The present invention is related to a novel enzymatic process for production of vitamin A alcohol (retinol) via conversion of retinal, which process includes the use of heterologous enzymes having activity as retinal reductase, particularly wherein the reaction leads to at least about 90% conversion of retinal into retinol. Said process is particularly useful for biotechnological production of vitamin A.

公开(公告)号：US20200231993A1

公开(公告)日：2020-07-23

申请号：US16647730

申请日：2018-09-25

Applicant: DSM IP ASSETS B.V.

Inventor： Nathalie BALCH ,  Paul BLOMQUIST ,  Reed DOTEN ,  Peter HOUSTON ,  Ethan LAM ,  Jenna MCMAHON ,  Joshua TRUEHEART ,  Celine VIAROUGE

IPC: C12P7/22 ,  C12N9/04 ,  C12N9/10 ,  C12P7/62

Abstract: The present invention is related to a novel enzymatic process for production of retinoids via a multi-step process, which process includes the use of heterologous enzymes having activity in a carotene-producing host cell, particularly wherein such process results in high percentage of retinoids, in trans-isoform.

公开(公告)号：US20200248151A1

公开(公告)日：2020-08-06

申请号：US16648851

申请日：2018-09-25

Applicant: DSM IP ASSETS B.V.

Inventor： Nathalie BALCH ,  Paul BLOMQUIST ,  Reed DOTEN ,  Peter HOUSTON ,  Ethan LAM ,  Jenna MCMAHON ,  Joshua TRUEHEART ,  Celine VIAROUGE

IPC: C12N9/04 ,  C12P23/00

Abstract: The present invention is related to a novel enzymatic process for production of vitamin A alcohol (retinol) via conversion of retinal, which process includes the use of heterologous enzymes having activity as retinal reductase, particularly wherein the reaction leads to at least about 90% conversion of retinal into retinol. Said process is particularly useful for biotechnological production of vitamin A.

公开(公告)号：US20200239924A1

公开(公告)日：2020-07-30

申请号：US16648872

申请日：2018-09-25

Applicant: DSM IP ASSETS B.V.

Inventor： Nathalie BALCH ,  Paul BLOMQUIST ,  Reed DOTEN ,  Peter HOUSTON ,  Ethan LAM ,  Jenna MCMAHON ,  Joshua TRUEHEART ,  Celine VIAROUGE

IPC: C12P23/00

Abstract: The present invention is related to a novel enzymatic process for production of retinyl esters, such as in particular retinyl long chain esters, via conversion of retinol, which process includes the use of enzymes having acyltransferase activity. Said process might be used for biotechnological production of vitamin A.

公开(公告)号：US20240279706A1

公开(公告)日：2024-08-22

申请号：US18292227

申请日：2021-07-27

Applicant: DSM IP ASSETS B.V.

Inventor： Peter Louis HOUSTON ,  Valmik Kanubhai VYAS ,  Anna SYMBOR-NAGRABSKA ,  Reed Chadbourne DOTEN ,  Christopher Mark FARRELL ,  Ethan LAM

IPC: C12P23/00 ,  C12N1/16 ,  C12N9/10

CPC classification number: C12P23/00 ,  C12N1/16 ,  C12N9/1029

Abstract: The present invention is related to a novel process for production of retinyl acetate in a host cell, particularly oleaginous yeast such as e.g. Yarrowia, wherein the product purity could be increased with reduction of unwanted side-products. Particularly, the novel process comprises fermentation in the presence of ethanol, such as e.g. in a fed-batch fermentation process. Such process is especially useful in a biotechnological process for production of vitamin A.

公开(公告)号：US20230265443A1

公开(公告)日：2023-08-24

申请号：US18017819

申请日：2021-07-22

Applicant: DSM IP ASSETS B.V.

Inventor： Peter Louis HOUSTON ,  Valmik Kanubhai VYAS ,  René Marcel DE JONG ,  Anna SYMBOR-NAGRABSKA ,  Ethan LAM

IPC: C12N15/81 ,  C12N1/16 ,  C12P23/00 ,  C12N9/20

CPC classification number: C12N15/815 ,  C12N1/16 ,  C12P23/00 ,  C12N9/20 ,  C12N2500/36 ,  C12Y301/01003

Abstract: The present invention is related to a novel process for production of retinyl acetate in a host cell, particularly oleaginous yeast such as e.g. Yarrowia, growing on triglyceride oils, such as e.g. vegetable oil, wherein the host cell exhibits modified lipase activity in such a way that conversion of retinol into fatty acid retinyl esters (FAREs) is reduced or abolished. Such process is especially useful in a biotechnological process for production of vitamin A.

公开(公告)号：US20230227862A1

公开(公告)日：2023-07-20

申请号：US18161236

申请日：2023-01-30

Applicant: DSM IP ASSETS B.V.

Inventor： Nathalie BALCH ,  Paul BLOMQUIST ,  Reed DOTEN ,  Peter HOUSTON ,  Ethan LAM ,  Jenna MCMAHON ,  Joshua TRUEHEART ,  Celine VIAROUGE

IPC: C12P7/22 ,  C12N9/04 ,  C12N9/10 ,  C12P7/62

CPC classification number: C12P7/22 ,  C12N9/0006 ,  C12N9/1029 ,  C12P7/62 ,  C12Y101/01105 ,  C12Y203/0102

Abstract: The present invention is related to a novel enzymatic process for production of retinoids via a multi-step process, which process includes the use of heterologous enzymes having activity in a carotene-producing host cell, particularly wherein such process results in high percentage of retinoids, in trans-isoform.

公开(公告)号：US20200239925A1

公开(公告)日：2020-07-30

申请号：US16648879

申请日：2018-09-25

Applicant: DSM IP ASSETS B.V.

Inventor： Nathalie BALCH ,  Paul BLOMQUIST ,  Reed DOTEN ,  Peter HOUSTON ,  Ethan LAM ,  Jenna MCMAHON ,  Joshua TRUEHEART ,  Celine VIAROUGE ,  René Marcel DE JONG

IPC: C12P23/00

Abstract: The present invention is related to production of retinyl esters, such as in particular retinyl acetate, an important building block for production of vitamin A. The retinyl esters might be generated via enzymatic conversion of retinol which process includes the use of enzymes with acetyl-transferase (ATF) activity, thus acetylating retinol into retinol/retinyl acetate. Said process is particularly useful for biotechnological production of vitamin A.