公开(公告)号：US11725223B2

公开(公告)日：2023-08-15

申请号：US16834756

申请日：2020-03-30

Applicant: DSM IP ASSETS B.V.

Inventor： Viktor Marius Boer ,  Erwin Suir

IPC: C12P19/56 ,  C12N9/88 ,  C12N9/02 ,  C12N9/16 ,  C12N15/52 ,  C12N9/90 ,  C07K14/39 ,  C12N9/10 ,  C12N9/24 ,  A23L27/30 ,  A23L2/60 ,  C07K14/395 ,  C12P15/00 ,  C07H15/256

CPC classification number: C12P19/56 ,  A23L2/60 ,  A23L27/36 ,  C07H15/256 ,  C07K14/39 ,  C07K14/395 ,  C12N9/0036 ,  C12N9/0042 ,  C12N9/0073 ,  C12N9/0083 ,  C12N9/1051 ,  C12N9/16 ,  C12N9/2405 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P15/00 ,  A23V2002/00

Abstract: The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol.

公开(公告)号：US10689681B2

公开(公告)日：2020-06-23

申请号：US14893216

申请日：2014-06-02

Applicant: DSM IP ASSETS B.V.

Inventor： Viktor Marius Boer ,  Erwin Suir

IPC: C12P19/56 ,  C12N9/88 ,  C12N9/02 ,  C12N9/16 ,  C12N15/52 ,  C12N9/90 ,  C07K14/39 ,  C12N9/10 ,  C12N9/24 ,  A23L27/30 ,  A23L2/60 ,  C07K14/395 ,  C12P15/00 ,  C07H15/256

Abstract: The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol. The recombinant microorganism may also be capable of expressing one or more UDP-glucosyltransferases such that the microorganism is capable of producing one or more steviol glycosides.