公开(公告)号：US10918113B2

公开(公告)日：2021-02-16

申请号：US16471159

申请日：2017-12-19

Applicant: DSM IP Assets B.V.

Inventor： Rene Marcel De Jong ,  Chantal Christis ,  Neil Carr

IPC: C12N15/00 ,  C12N1/20 ,  C12N9/16 ,  C12N9/18 ,  A21D8/04 ,  C12N9/20 ,  C12N15/52

Abstract: The present disclosure describes a variant polypeptide having lipolytic activity, wherein the variant has an amino acid sequence which, when aligned with the amino acid sequence as set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue at a position corresponding to any of the positions 53, 112, 141, 178, 179, 182, 202, 203, 235, 282, 284,
said positions being defined with reference to SEQ ID NO: 2, and wherein said variant has at least 70% identity with the mature polypeptide having lipolytic activity as set out in SEQ ID NO: 2.
Such a variant polypeptide may be used in the preparation of a baked product.

公开(公告)号：US11667902B2

公开(公告)日：2023-06-06

申请号：US17109759

申请日：2020-12-02

Applicant: DSM IP ASSETS B.V.

Inventor： Rene Marcel De Jong ,  Chantal Christis ,  Neil Carr

IPC: C12N9/20 ,  A21D8/04

CPC classification number: C12N9/20 ,  A21D8/042 ,  C12Y301/01003

Abstract: The present disclosure describes a variant polypeptide having lipolytic activity, wherein the variant has an amino acid sequence which, when aligned with the amino acid sequence as set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue at a position corresponding to any of the positions 113, 122, 138, 141, 179, 282, 284, 286, 295, said positions being defined with reference to SEQ ID NO: 2, and wherein said variant has at least 70% identity with the mature polypeptide having lipolytic activity as set out in SEQ ID NO: 2. Such a variant polypeptide may be used in the preparation of a baked product.

公开(公告)号：US12195769B2

公开(公告)日：2025-01-14

申请号：US17253266

申请日：2019-06-18

Applicant: DSM IP ASSETS B.V.

Inventor： Joke Anneleen Putseys ,  Neil Carr ,  René Marcel De Jong ,  Aloysius Wilhelmus Rudolphus Hubertus Teunissen

IPC: C12N15/00 ,  A21D6/00 ,  A21D8/04 ,  C12N9/16 ,  C12N9/18 ,  C12N9/20

Abstract: The present invention relates to a variant polypeptide having a lipase activity, wherein the polypeptide may be a polypeptide that has an amino acid sequence which has at least 70% identity to a mature amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 6, wherein the polypeptide comprises an amino acid substitution at position 295 and at least one further amino acid substitution at position 113, 121, 179 and/or 284, wherein the positions are defined with reference to SEQ ID NO: 1 or SEQ ID NO: 6. The invention further relates to a process for preparing a dough wherein a polypeptide as disclosed herein is used and baked product prepared from the dough.

公开(公告)号：US10889807B2

公开(公告)日：2021-01-12

申请号：US16471121

申请日：2017-12-19

Applicant: DSM IP Assets B.V.

Inventor： Rene Marcel De Jong ,  Chantal Christis ,  Neil Carr

IPC: C12N15/00 ,  C12N9/16 ,  C12N9/18 ,  A21D8/04 ,  C12N9/20

Abstract: The present disclosure describes a variant polypeptide having lipolytic activity, wherein the variant has an amino acid sequence which, when aligned with the amino acid sequence as set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue at a position corresponding to any of the positions 113, 122, 138, 141, 179, 282, 284, 286, 295,
said positions being defined with reference to SEQ ID NO: 2, and wherein said variant has at least 70% identity with the mature polypeptide having lipolytic activity as set out in SEQ ID NO: 2.
Such a variant polypeptide may be used in the preparation of a baked product.