公开(公告)号：US10450554B2

公开(公告)日：2019-10-22

申请号：US15312171

申请日：2015-05-19

Applicant: DSM IP ASSETS B.V.

Inventor： Jan Metske Van Der Laan ,  Peter Jozef Ida Van De Vondervoort ,  Chantal Christis ,  Martine Spaans ,  Angela De Bruine-Paulus ,  Johanna Henrica Gerdina Maria Mutsaers

IPC: A61K38/48 ,  C12N1/20 ,  C12N9/62 ,  A23L2/84 ,  A23J3/34 ,  C12N9/50 ,  A23L2/66 ,  A23L7/10 ,  A23L33/18 ,  A23L33/195

Abstract: The present invention relates to a polypeptide having proline-specific endoprotease activity, selected from the group consisting of i. a polypeptide comprising a mature polypeptide sequence of SEQ ID NO: 2; ii. a polypeptide that has least 70%, 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identity to the mature polypeptide sequence of SEQ ID NO: 2; iii. a polypeptide encoded by a nucleic acid that hybridizes under medium stringency, preferably under high stringency conditions to the complementary strand of the mature polypeptide coding sequence of SEQ ID NO:1; iv. a polypeptide encoded by a nucleic acid that has at least 70%, 75% 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% identity to the mature polypeptide coding sequence of SEQ ID NO: 1. The invention further relates to a method of producing the polypeptide and a process for the preparation of a food or feed product wherein the polypeptide is used.

公开(公告)号：US10918113B2

公开(公告)日：2021-02-16

申请号：US16471159

申请日：2017-12-19

Applicant: DSM IP Assets B.V.

Inventor： Rene Marcel De Jong ,  Chantal Christis ,  Neil Carr

IPC: C12N15/00 ,  C12N1/20 ,  C12N9/16 ,  C12N9/18 ,  A21D8/04 ,  C12N9/20 ,  C12N15/52

Abstract: The present disclosure describes a variant polypeptide having lipolytic activity, wherein the variant has an amino acid sequence which, when aligned with the amino acid sequence as set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue at a position corresponding to any of the positions 53, 112, 141, 178, 179, 182, 202, 203, 235, 282, 284,
said positions being defined with reference to SEQ ID NO: 2, and wherein said variant has at least 70% identity with the mature polypeptide having lipolytic activity as set out in SEQ ID NO: 2.
Such a variant polypeptide may be used in the preparation of a baked product.

公开(公告)号：US11667902B2

公开(公告)日：2023-06-06

申请号：US17109759

申请日：2020-12-02

Applicant: DSM IP ASSETS B.V.

Inventor： Rene Marcel De Jong ,  Chantal Christis ,  Neil Carr

IPC: C12N9/20 ,  A21D8/04

CPC classification number: C12N9/20 ,  A21D8/042 ,  C12Y301/01003

Abstract: The present disclosure describes a variant polypeptide having lipolytic activity, wherein the variant has an amino acid sequence which, when aligned with the amino acid sequence as set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue at a position corresponding to any of the positions 113, 122, 138, 141, 179, 282, 284, 286, 295, said positions being defined with reference to SEQ ID NO: 2, and wherein said variant has at least 70% identity with the mature polypeptide having lipolytic activity as set out in SEQ ID NO: 2. Such a variant polypeptide may be used in the preparation of a baked product.

公开(公告)号：US10202594B2

公开(公告)日：2019-02-12

申请号：US15314892

申请日：2015-06-03

Applicant: DSM IP ASSETS B.V.

Inventor： Jan Metske Van Der Laan ,  Peter Jozef Ida Van De Vondervoort ,  Chantal Christis ,  Martine Spaans ,  Angela De Bruine-Paulus

IPC: C12N9/50 ,  C12N9/62

Abstract: The present invention relates to a polypeptide having proline-specific endoprotease activity, wherein the polypeptide has less than 70% residual activity when the polypeptide has been kept at a temperature of 65° C. for 15 min.The invention further relates to a polypeptide having proline-specific endoprotease activity comprising an amino acid sequence according to SEQ ID NO: 1, wherein SEQ ID NO: 1 comprises at least one combination of amino acid substitutions selected from the group consisting of a combination selected from the group consisting of (K238E, I204V, V460A), (F279S, A242V, N507I), (T145A, K424M), (T359A, F379S), (M170I, A421T), (N441S, G484S), (L470H, Q288R), (L470H, E387G), (T281S, L373I), (P304A, P469A), and (P466T, P469Q), a nucleic acid encoding a polypeptide having proline-specific endoprotease activity, a method of making a variant polypeptide having proline-specific endoprotease activity, a recombinant host cell and a method of producing the polypeptide and a process for the preparation of a food or feed product wherein the polypeptide is used.

公开(公告)号：US10889807B2

公开(公告)日：2021-01-12

申请号：US16471121

申请日：2017-12-19

Applicant: DSM IP Assets B.V.

Inventor： Rene Marcel De Jong ,  Chantal Christis ,  Neil Carr

IPC: C12N15/00 ,  C12N9/16 ,  C12N9/18 ,  A21D8/04 ,  C12N9/20

Abstract: The present disclosure describes a variant polypeptide having lipolytic activity, wherein the variant has an amino acid sequence which, when aligned with the amino acid sequence as set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue at a position corresponding to any of the positions 113, 122, 138, 141, 179, 282, 284, 286, 295,
said positions being defined with reference to SEQ ID NO: 2, and wherein said variant has at least 70% identity with the mature polypeptide having lipolytic activity as set out in SEQ ID NO: 2.
Such a variant polypeptide may be used in the preparation of a baked product.

公开(公告)号：US10294467B2

公开(公告)日：2019-05-21

申请号：US15314825

申请日：2015-06-03

Applicant: DSM IP ASSETS B.V.

Inventor： Jan Metske Van Der Laan ,  Peter Jozef Ida Van De Vondervoort ,  Chantal Christis ,  Martine Spaans ,  Angela De Bruine-Paulus

IPC: C12N9/62 ,  C12C11/00

Abstract: The present invention relates to a polypeptide having proline-specific endoprotease activity, wherein the polypeptide has less than 70% residual activity when the polypeptide has been kept at a temperature of 65° C. for 15 min.The invention further relates to a polypeptide having proline-specific endoprotease activity comprising an amino acid sequence according to SEQ ID NO: 1, wherein SEQ ID NO: 1 comprises at least one amino acid substitution selected from the group consisting of P469A, P469C, P469D, P469E, P469F, P469G, P469H, P469I, P469K, P469L, P469M, P469N, P469Q, P469R, P469S, P469T, P469V, P469W, P469Y, a nucleic acid encoding a polypeptide having proline-specific endoprotease activity, a method of making a variant polypeptide having proline-specific endoprotease activity, a recombinant host cell and a method of producing the polypeptide and a process for the preparation of a food or feed product wherein the polypeptide is used.