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    Dewaxing kit for immunostaining tissue specimens
    4.
    发明授权
    Dewaxing kit for immunostaining tissue specimens 失效
    用于免疫染色组织标本的脱蜡试剂盒

    公开(公告)号：US07070951B2

    公开(公告)日：2006-07-04

    申请号：US10652854

    申请日：2003-08-29

    申请人： Guangrong Zhang ,  Cheng-Zhi Yu ,  Sheng-Hui Su ,  Krishan L. Kalra ,  Ding Zhou

    发明人： Guangrong Zhang ,  Cheng-Zhi Yu ,  Sheng-Hui Su ,  Krishan L. Kalra ,  Ding Zhou

    IPC分类号： G01N1/30

    CPC分类号： C11D1/143 ,  C11D1/62 ,  C11D1/72 ,  C11D3/187 ,  C11D3/188 ,  C11D3/201 ,  C11D3/2044 ,  G01N1/30 ,  G01N1/36 ,  Y10S435/975

    摘要： Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use, and maintaining compatibility with downstream histochemical analyses, particularly immunostaining. Compositions of the invention comprise a paraffin-solubilizing organic solvent, a polar organic solvent, and a surfactant. Compositions can further comprise water. The method involves contacting a wax-embedded specimen with the dewaxing composition to solubilize the wax impregnating the specimen prior to histochemical analysis. The method can comprise the further step of washing the dewaxed specimen immediately after dewaxing with an aqueous wash composition comprising a detergent to remove residual dewaxing composition. Also provided is a kit for dewaxing a wax-embedded specimen, which comprises a dewaxing composition and can further comprise a second composition of (1) an immunostaining reagent or (2) an aqueous wash solution comprising a detergent for removing residual dewaxing solution.

    摘要翻译： 提供组合物和方法，用于在组织化学分析前对蜡嵌入的生物样品进行脱蜡。 所提供的组合物和方法可以在用于组织化学或其他诊断分析的准备期间有效地从标本中去除蜡或改进的蜡基嵌入材料，特别是基于石蜡的包埋材料，同时最小化对使用者的危害，实现与自动使用的兼容性并保持与下游的兼容性 组织化学分析，特别是免疫染色。 本发明的组合物包含石蜡溶解性有机溶剂，极性有机溶剂和表面活性剂。 组合物还可以包含水。 该方法包括使蜡包埋的样品与脱蜡组合物接触，以在组织化学分析之前溶解浸渍样品的蜡。 该方法可以包括在用包含洗涤剂的水性洗涤组合物脱蜡之后立即洗涤脱蜡样品以除去残余脱蜡组合物的步骤。 还提供了一种用于脱蜡蜡包埋样品的试剂盒，其包含脱蜡组合物，并且还可以包含（1）免疫染色试剂的第二组合物或（2）包含用于除去残余脱蜡溶液的洗涤剂的水性洗涤溶液。

    Biotinylated oligonucleotides and reagents for preparing the same
    5.
    发明授权
    Biotinylated oligonucleotides and reagents for preparing the same 失效
    生物素化寡核苷酸及其制备方法

    公开(公告)号：US5128476A

    公开(公告)日：1992-07-07

    申请号：US658242

    申请日：1991-02-20

    申请人： Guangrong Zhang ,  Richard V. Case

    发明人： Guangrong Zhang ,  Richard V. Case

    IPC分类号： C07F9/6561 ,  C07H21/00

    CPC分类号： C07H21/00 ,  C07F9/6561

    摘要： Reagents of the formula I ##STR1## wherein A is --CH.sub.2 --.sub.m O-- (m=2 to 20) or --C.sub.n H.sub.2n --O--.sub.t (n=2 to 6; t=2 to 10) and B is selected from the group consisting of ##STR2## and salts thereof are useful for the preparation of 5'-biotinylated oligonucleotides.

    摘要翻译： 式I的试剂（I）其中A是-CH 2 -mO-（m = 2〜20）或-C n H 2n-Ot（n = 2-6），t = 2〜10，B选自 由及其盐组成的组可用于制备5'-生物素化的寡核苷酸。

    Detritylation solvents for nucleic acid synthesis
    6.
    发明授权
    Detritylation solvents for nucleic acid synthesis 有权
    用于核酸合成的去离子溶剂

    公开(公告)号：US06538128B1

    公开(公告)日：2003-03-25

    申请号：US09148344

    申请日：1999-03-22

    申请人： Guangrong Zhang ,  Jin-Yan Tang

    发明人： Guangrong Zhang ,  Jin-Yan Tang

    IPC分类号： C07H2100

    CPC分类号： C07H21/00

    摘要： The invention provides improved processes for oligonucleotide synthesis utilizing arenes as solvents for detritylation of oligonucleotides.

    摘要翻译： 本发明提供用于寡核苷酸合成的改进方法，所述方法利用芳烃作为寡核苷酸不稳定性的溶剂。

    Deparaffinization compositions for dewaxing tissue specimens
    7.
    发明申请
    Deparaffinization compositions for dewaxing tissue specimens 审中-公开
    用于脱蜡组织标本的脱蜡组合物

    公开(公告)号：US20060019332A1

    公开(公告)日：2006-01-26

    申请号：US11186392

    申请日：2005-07-20

    申请人： Guangrong Zhang ,  Cheng-Zhi Yu ,  Sheng-Hui Su ,  Krishan Kalra ,  Ding Zhou

    发明人： Guangrong Zhang ,  Cheng-Zhi Yu ,  Sheng-Hui Su ,  Krishan Kalra ,  Ding Zhou

    IPC分类号： G01N1/30 ,  G01N33/48 ,  C23G5/00

    CPC分类号： C11D1/143 ,  C11D1/62 ,  C11D1/72 ,  C11D3/187 ,  C11D3/188 ,  C11D3/201 ,  C11D3/2044 ,  G01N1/30 ,  G01N1/36 ,  Y10S435/975

    摘要： Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use, and maintaining compatibility with downstream histochemical analyses, particularly immunostaining. Compositions of the invention comprise a paraffin-solubilizing organic solvent, a polar organic solvent, and a surfactant. Compositions can further comprise water. The method involves contacting a wax-embedded specimen with the dewaxing composition to solubilize the wax impregnating the specimen prior to histochemical analysis. The method can comprise the further step of washing the dewaxed specimen immediately after dewaxing with an aqueous wash composition comprising a detergent to remove residual dewaxing composition. Also provided is a kit for dewaxing a wax-embedded specimen, which comprises a dewaxing composition and can further comprise a second composition of (1) an immunostaining reagent or (2) an aqueous wash solution comprising a detergent for removing residual dewaxing solution.

    摘要翻译： 提供组合物和方法，用于在组织化学分析前对蜡嵌入的生物样品进行脱蜡。 所提供的组合物和方法可以在用于组织化学或其他诊断分析的准备期间有效地从标本中去除蜡或改进的蜡基嵌入材料，特别是基于石蜡的包埋材料，同时最小化对使用者的危害，实现与自动使用的兼容性并保持与下游的兼容性 组织化学分析，特别是免疫染色。 本发明的组合物包含石蜡溶解性有机溶剂，极性有机溶剂和表面活性剂。 组合物还可以包含水。 该方法包括使蜡包埋的样品与脱蜡组合物接触，以在组织化学分析之前溶解浸渍样品的蜡。 该方法可以包括在用包含洗涤剂的水性洗涤组合物脱蜡之后立即洗涤脱蜡样品以除去残余脱蜡组合物的步骤。 还提供了一种用于脱蜡蜡包埋样品的试剂盒，其包含脱蜡组合物，并且还可以包含（1）免疫染色试剂的第二组合物或（2）包含用于除去残余脱蜡溶液的洗涤剂的水性洗涤溶液。

    Deparaffinization compositions and methods for their use
    8.
    发明授权
    Deparaffinization compositions and methods for their use 失效
    脱蜡组合物及其使用方法

    公开(公告)号：US06632598B1

    公开(公告)日：2003-10-14

    申请号：US08212175

    申请日：1994-03-11

    申请人： Guangrong Zhang ,  Cheng-Zhi Yu ,  Sheng-Hui Su ,  Krishan L. Kalra ,  Ding Zhou

    发明人： Guangrong Zhang ,  Cheng-Zhi Yu ,  Sheng-Hui Su ,  Krishan L. Kalra ,  Ding Zhou

    IPC分类号： C12Q100

    CPC分类号： C11D1/143 ,  C11D1/62 ,  C11D1/72 ,  C11D3/187 ,  C11D3/188 ,  C11D3/201 ,  C11D3/2044 ,  G01N1/30 ,  G01N1/36 ,  Y10S435/975

    摘要： Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use, and maintaining compatibility with downstream histochemical analyses, particularly immunostaining. Compositions of the invention comprise a paraffin-solubilizing organic solvent, a polar organic solvent, and a surfactant. Compositions can further comprise water. The method involves contacting a wax-embedded specimen with the dewaxing composition to solubilize the wax impregnating the specimen prior to histochemical analysis. The method can comprise the further step of washing the dewaxed specimen immediately after dewaxing with an aqueous wash composition comprising a detergent to remove residual dewaxing composition. Also provided is a kit for dewaxing a wax-embedded specimen, which comprises a dewaxing composition and can further comprise a second composition of (1) an immunostaining reagent or (2) an aqueous wash solution comprising a detergent for removing residual dewaxing solution.

    摘要翻译： 提供组合物和方法，用于在组织化学分析前对蜡嵌入的生物样品进行脱蜡。 所提供的组合物和方法可以在用于组织化学或其他诊断分析的准备期间有效地从标本中去除蜡或改进的蜡基嵌入材料，特别是基于石蜡的包埋材料，同时最小化对使用者的危害，实现与自动使用的兼容性并保持与下游的兼容性 组织化学分析，特别是免疫染色。 本发明的组合物包含石蜡溶解性有机溶剂，极性有机溶剂和表面活性剂。 组合物还可以包含水。 该方法包括使蜡包埋的样品与脱蜡组合物接触，以在组织化学分析之前溶解浸渍样品的蜡。 该方法可以包括在用包含洗涤剂的水性洗涤组合物脱蜡之后立即洗涤脱蜡样品以除去残余脱蜡组合物的步骤。 还提供了一种用于脱蜡蜡包埋样品的试剂盒，其包含脱蜡组合物，并且还可以包含第二组合物（1）免疫染色试剂或（2）包含用于除去残余脱蜡溶液的洗涤剂的水性洗涤溶液。 / PTEXT>