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    Functional expression of mammalian adenylyl cyclase in yeast
    5.
    发明授权
    Functional expression of mammalian adenylyl cyclase in yeast 失效
    酵母中哺乳动物腺苷酸环化酶的功能表达

    公开(公告)号：US6001553A

    公开(公告)日：1999-12-14

    申请号：US732218

    申请日：1997-01-14

    申请人： James R. Broach ,  John P. Manfredi ,  Joshua Trueheart

    发明人： James R. Broach ,  John P. Manfredi ,  Joshua Trueheart

    IPC分类号： G01N33/573 ,  C12N1/19 ,  C12N9/88 ,  C12N15/09 ,  C12N15/60 ,  C12Q1/02 ,  C12Q1/00 ,  C12N1/14

    CPC分类号： C12N9/88

    摘要： Mammalian adenylyl cyclases are functionally expressed in yeast cells. The yeast cells may be used to screen for inhibitors or activators of the adenylyl cyclase, or of a regulator of adenylyl cyclase which is functionally co-expressed in the yeast cell. Methods of identifying such inhibitors, activators and regulators are also disclosed.

    摘要翻译： PCT No.PCT / US95 / 05149 Sec。 371日期1997年1月14日 102（e）日期1997年1月14日PCT 1995年4月26日PCT PCT。 第WO95 / 30012号公报 日期：1995年11月9日玛氏腺苷酸环化酶在酵母细胞中功能表达。 酵母细胞可用于筛选腺苷酸环化酶的抑制剂或活化剂，或在酵母细胞中功能共表达的腺苷酸环化酶的调节剂。 还公开了鉴定这种抑制剂，活化剂和调节剂的方法。

    Cell having amplified signal transduction pathway responses and uses therefor
    6.
    发明授权
    Cell having amplified signal transduction pathway responses and uses therefor 失效
    细胞具有扩增的信号转导途径反应和用途

    公开(公告)号：US07273747B2

    公开(公告)日：2007-09-25

    申请号：US10267074

    申请日：2002-10-08

    申请人： John P. Manfredi ,  Benjamin K. Benton ,  Meng-Yu Wu

    发明人： John P. Manfredi ,  Benjamin K. Benton ,  Meng-Yu Wu

    IPC分类号： C12N1/19 ,  C12N15/81 ,  G01N33/554

    CPC分类号： C12N15/81 ,  C07K14/723

    摘要： The invention provides recombinant cells that have been engineered such that ligand stimulation of a receptor expressed by the cells leads to amplified signal transduction responses. In one embodiment, the receptor-expressing cells have been engineered to carry a heterologous DNA construct comprising a gene encoding a protein that activates the signal transduction pathway, which gene is operatively linked to a promoter that is responsive to activation of the signal transduction pathway. Stimulation of the receptor by a ligand leads to expression of the heterologous DNA construct encoding the protein that activates the signal transduction pathway such that signals generated by ligand binding to the receptor are amplified. Preferred cells are yeast cells expressing heterologous G protein coupled receptors functionally coupled to the yeast pheromone response pathway and overexpressing Ste5p, Ste4p, Ste12p, Ste11p or a dominant truncation allele of Ste20 via a pheromone-responsive promoter. The invention further provides cells expressing a heterologous receptor, wherein an endogenous gene encoding a protein that negatively regulates an endogenous signal transduction pathway is mutated to render the protein nonfunctional such that signals generated by ligand binding to the receptor are amplified. The invention further provides cells expressing a heterologous receptor, wherein an endogenous gene encoding a protein that positively regulates an endogenous signal transduction pathway is mutated to a supersensitive form such that the sensitivity of the pathway to ligand stimulation is increases. Methods of using the cells of the invention to identify receptor modulators are also provided.

    摘要翻译： 本发明提供重组细胞，这些细胞已经被改造成使得由细胞表达的受体的配体刺激导致扩增的信号转导反应。 在一个实施方案中，受体表达细胞已被改造以携带包含编码活化信号转导途径的蛋白质的基因的异源DNA构建体，该基因可操作地连接于对信号转导途径的激活有反应的启动子。 通过配体刺激受体导致编码激活信号转导途径的蛋白质的异源DNA构建体的表达，使得通过配体结合受体产生的信号被扩增。 优选的细胞是表达与酵母信息素反应途径功能耦合的异源G蛋白偶联受体的酵母细胞，并且通过信息素反应启动子过表达Ste5p，Ste4p，Ste12p，Ste11p或Ste20的显性截短等位基因。 本发明还提供了表达异源受体的细胞，其中编码负调节内源信号转导途径的蛋白质的内源基因被突变以使蛋白质无功能化，使得通过配体结合受体产生的信号被扩增。 本发明进一步提供表达异源受体的细胞，其中编码将内源性信号转导途径正调节的蛋白质的内源基因突变为超敏感形式，使得该途径对配体刺激的敏感性增加。 还提供了使用本发明的细胞鉴定受体调节剂的方法。

    METHODS AND COMPOSITIONS FOR NEMATODE GROWTH
    7.
    发明申请
    METHODS AND COMPOSITIONS FOR NEMATODE GROWTH 审中-公开
    NEMATODE GROWTH的方法和组合物

    公开(公告)号：US20120325152A1

    公开(公告)日：2012-12-27

    申请号：US13531039

    申请日：2012-06-22

    申请人： Christopher E. Hopkins ,  John P. Manfredi

    发明人： Christopher E. Hopkins ,  John P. Manfredi

    IPC分类号： A01K67/033 ,  A23L1/48

    CPC分类号： A01K67/033

    摘要： Compositions for nematode growth and methods for using the same for nematode viability are described. The compositions contain a solid matrix material that is dispersed in a liquid medium. The solid matrix can be immersed, bathed, and/or interspersed in a liquid medium to form a composite culture that is capable of maintaining or growing nematodes at unexpectedly high densities and/or with nominal stress pathway activity. Other embodiments are described.

    摘要翻译： 描述了用于线虫生长的组合物及其用于线虫活力的方法。 组合物含有分散在液体介质中的固体基质材料。 可以将固体基质浸入，洗涤和/或散布在液体培养基中以形成能够以意想不到的高密度和/或具有标称应力通路活性保持或生长线虫的复合培养物。 描述其他实施例。