公开(公告)号：US20050239122A1

公开(公告)日：2005-10-27

申请号：US11147804

申请日：2005-06-07

申请人： Daniel Afar ,  Rene Hubert ,  Kahan Leong ,  Arthur Raitano ,  Douglas Saffran ,  Stephen Mitchell ,  Aya Jakobovits ,  Mary Faris ,  Igor Vivanco

发明人： Daniel Afar ,  Rene Hubert ,  Kahan Leong ,  Arthur Raitano ,  Douglas Saffran ,  Stephen Mitchell ,  Aya Jakobovits ,  Mary Faris ,  Igor Vivanco

IPC分类号： C12Q1/68 ,  G01N33/574

CPC分类号： C12Q1/6886 ,  C12Q2600/136 ,  C12Q2600/154 ,  G01N33/57419 ,  G01N33/57434 ,  G01N33/57488 ,  G01N2333/95

摘要： Compositions for the diagnosis and therapy of prostate and colon cancer, derived from or based on a novel prostate-specific, androgen-regulated, cell membrane associated and secreted serine protease termed 20P1F12/TMPRSS2 are described. A full length cDNA comprising the entire coding sequence of the 20P1F12/TMPRSS2 gene (also designated 20P1F12-GTC1 herein) is provided (FIG. 1). Among the compositions provided are antibodies that bind to 20P1F12/TMPRSS2 proteins and polypeptide fragments thereof, including antibodies labeled with a detectable marker or toxin or therapeutic composition. The invention also provides prognostic and diagnostic methods of examining a biological sample for evidence of disregulated cellular growth by comparing the status of 20P1F12/TMPRSS2 in the biological sample to the status of 20P1F12/TMPRSS2 in a corresponding normal sample, wherein alterations in the status of 20P1F12/TMPRSS2 in the biological sample are associated with disregulated cellular growth. The invention further provides various therapeutic compositions and strategies for treating prostate cancer, including particularly, 20P1F12/TMPRSS2 polypeptide and anti-20P1F12/TMPRSS2 antibody therapy methods and compositions, cancer vaccines, and small molecule therapy.

摘要翻译： 描述了衍生自或基于称为20P1F12 / TMPRSS2的新型前列腺特异性，雄激素调节的细胞膜相关和分泌的丝氨酸蛋白酶的前列腺和结肠癌的诊断和治疗的组合物。 提供了包含20P1F12 / TMPRSS2基因（本文中也称为20P1F12-GTC1）的整个编码序列的全长cDNA（图1）。 所提供的组合物是结合20P1F12 / TMPRSS2蛋白和其多肽片段的抗体，包括用可检测标记或毒素或治疗组合物标记的抗体。 本发明还提供了通过将生物样品中20P1F12 / TMPRSS2的状态与对应的正常样品中的20P1F12 / TMPRSS2的状态进行比较来检查生物样品以检测细胞生长失调的证据的预后和诊断方法，其中， 生物样品中的20P1F12 / TMPRSS2与细胞生长失调有关。 本发明还提供了治疗前列腺癌的各种治疗组合物和策略，特别包括20P1F12 / TMPRSS2多肽和抗-20P1F12 / TMPRSS2抗体治疗方法和组合物，癌症疫苗和小分子治疗。

公开(公告)号：US20050148012A1

公开(公告)日：2005-07-07

申请号：US11061341

申请日：2005-02-18

申请人： Daniel Afar ,  Rene Hubert ,  Kahan Leong ,  Arthur Raitano ,  Douglas Saffran ,  Aya Jakobovits

发明人： Daniel Afar ,  Rene Hubert ,  Kahan Leong ,  Arthur Raitano ,  Douglas Saffran ,  Aya Jakobovits

IPC分类号： G01N33/493 ,  A61K31/7088 ,  A61K31/7105 ,  A61K39/00 ,  A61K39/385 ,  A61K39/395 ,  A61K48/00 ,  A61P13/08 ,  A61P13/10 ,  A61P35/00 ,  C07K14/47 ,  C07K16/18 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/68 ,  C12R1/91 ,  G01N33/50 ,  G01N33/53 ,  C07K16/30 ,  C07K16/46 ,  G01N33/574

CPC分类号： C07K14/47 ,  A61K39/00 ,  A61K48/00 ,  C07K2317/24 ,  C07K2319/02 ,  C12N2799/026 ,  C12N2799/027

摘要： Described is a novel gene and its encoded secreted tumor antigen, termed BPC-1, and to diagnostic and therapeutic methods and compositions useful in the management of various cancers which express BPC-1, particularly including prostate cancer and bladder cancer. In human normal tissues, BPC-1 is only expressed in certain tissues of the brain. However, BPC-1 is expressed at high levels in prostate cancer cells and is also expressed in bladder cancer cells. The structure of BPC-1 includes a signal sequence and a CUB domain. BPC-1 protein is secreted. Preliminary experimental evidence suggests that BPC-1 is directly involved in oncogenesis or maintenance of the transformed phenotype of cancer cells expressing BPC-1. BPC-1 also appears to bind specifically to a cellular protein expressed in prostate cancer cells and other cells.

摘要翻译： 描述了一种新型基因及其编码的分泌的肿瘤抗原，称为BPC-1，以及用于治疗表达BPC-1的各种癌症的诊断和治疗方法和组合物，特别包括前列腺癌和膀胱癌。 在人正常组织中，BPC-1仅在脑的某些组织中表达。 然而，BPC-1在前列腺癌细胞中以高水平表达，并且也在膀胱癌细胞中表达。 BPC-1的结构包括信号序列和CUB域。 分泌BPC-1蛋白。 初步实验证据表明，BPC-1直接参与肿瘤发生或维持表达BPC-1的癌细胞的转化表型。 BPC-1也似乎特异性结合前列腺癌细胞和其他细胞中表达的细胞蛋白。

公开(公告)号：US20070104720A1

公开(公告)日：2007-05-10

申请号：US11580538

申请日：2006-10-12

申请人： Daniel Afar ,  Rene Hubert ,  Kahan Leong ,  Arthur Raitano ,  Douglas Saffran ,  Stephen Mitchell

发明人： Daniel Afar ,  Rene Hubert ,  Kahan Leong ,  Arthur Raitano ,  Douglas Saffran ,  Stephen Mitchell

IPC分类号： A61K39/395 ,  C07H21/04 ,  C12P21/06 ,  C07K14/82 ,  C07K16/30 ,  C07K16/46

CPC分类号： C07K14/4748 ,  C07K14/705 ,  C07K14/723 ,  C07K16/28 ,  C07K16/3069 ,  C07K2317/34 ,  C07K2319/00 ,  Y10T436/143333

摘要： Described is a novel family of cell surface serpentine transmembrane antigens. Two of the proteins in this family are exclusively or predominantly expressed in the prostate, as well as in prostate cancer, and thus members of this family have been termed “STEAP” (SIX Transmembrane Epithelial Antigens of the Prostate). Four particular human STEAPs are described and characterized herein. The human STEAPs exhibit a high degree of structural conservation among them but show no significant structural homology to any known human proteins. The prototype member of the STEAP family, STEAP-1, appears to be a type IIIa membrane protein expressed predominantly in prostate cells in normal human tissues. Structurally, STEAP-1 is a 339 amino acid protein characterized by a molecular topology of six transmembrane domains and intracellular N— and C-termini, suggesting that it folds in a “serpentine” manner into three extracellular and two intracellular loops. STEAP-1 protein expression is maintained at high levels across various stages of prostate cancer. Moreover, STEAP-1 is highly over-expressed in certain other human cancers.

摘要翻译： 描述了一种新型的细胞表面蛇纹石跨膜抗原家族。 该家族中的两种蛋白质仅在或主要地在前列腺中表达，并且在前列腺癌中，因此该家族的成员被称为“STEAP”（前列腺的第六跨膜上皮原体）。 本文描述和表征了四种特定的人STEAP。 人类STEAP在其中表现出高度的结构保守性，但与任何已知的人类蛋白质没有显着的结构同源性。 STEAP家族的原型成员STEAP-1似乎是主要在正常人体组织中的前列腺细胞中表达的IIIa型膜蛋白。 在结构上，STEAP-1是一种339个氨基酸的蛋白质，其特征在于六个跨膜结构域和细胞内N-和C-末端的分子拓扑结构，表明它以“蛇纹石”的方式折叠成三个胞外和两个细胞内环。 STEAP-1蛋白表达在前列腺癌的各个阶段保持在高水平。 此外，STEAP-1在某些其他人类癌症中高度过度表达。

公开(公告)号：US20060275304A1

公开(公告)日：2006-12-07

申请号：US11200973

申请日：2005-08-09

申请人： Daniel Afar ,  Rene Hubert ,  Kahan Leong ,  Arthur Raitano ,  Douglas Saffran ,  Stephen Mitchell

发明人： Daniel Afar ,  Rene Hubert ,  Kahan Leong ,  Arthur Raitano ,  Douglas Saffran ,  Stephen Mitchell

IPC分类号： A61K39/395 ,  C12Q1/68 ,  C07H21/04 ,  C12P21/06 ,  C07K16/30 ,  C07K14/82 ,  C07K16/46

CPC分类号： C07K16/40 ,  A61K38/00 ,  C12N9/6421

摘要： Compositions for the diagnosis and therapy of prostate and colon cancer, derived from or based on a novel prostate-specific, androgen-regulated, cell surface serine protease termed 20P1F12/TMPRSS2 are described. A full length cDNA comprising the entire coding sequence of the 20P1F12/TMPRSS2 gene (also designated 20P1F12-GTC1 herein) is provided (FIG. 1). Among the compositions provides are antibodies that bind to 20P1F12/TMPRSS2 proteins and polypeptide fragments thereof, including antibodies labeled with a detectable marker or toxin or therapeutic composition. Several monoclonal antibodies specifically reactive with 20P1F12/TMPRSS2 are also described herein.

摘要翻译： 描述了源自或基于称为20P1F12 / TMPRSS2的新型前列腺特异性，雄激素调节的细胞表面丝氨酸蛋白酶的前列腺和结肠癌的诊断和治疗的组合物。 提供了包含20P1F12 / TMPRSS2基因（本文中也称为20P1F12-GTC1）的整个编码序列的全长cDNA（图1）。 组合物中提供的是与20P1F12 / TMPRSS2蛋白及其多肽片段结合的抗体，包括用可检测标记或毒素或治疗组合物标记的抗体。 本文还描述了与20P1F12 / TMPRSS2特异性反应的几种单克隆抗体。

公开(公告)号：US20050202454A1

公开(公告)日：2005-09-15

申请号：US10858887

申请日：2004-06-01

申请人： Daniel Afar ,  Rene Hubert ,  Kahan Leong ,  Arthur Raitano ,  Douglas Saffran ,  Stephen Mitchell

发明人： Daniel Afar ,  Rene Hubert ,  Kahan Leong ,  Arthur Raitano ,  Douglas Saffran ,  Stephen Mitchell

IPC分类号： G01N33/574 ,  A61K38/00 ,  A61K39/395 ,  A61P35/00 ,  C07K14/47 ,  C07K14/705 ,  C07K16/18 ,  C12N5/10 ,  C12N15/09 ,  C12P21/00 ,  C12P21/08 ,  C12Q1/68 ,  C07H21/04 ,  C07K14/82 ,  C07K16/30

CPC分类号： C07K14/4748 ,  C07K14/705 ,  C07K14/723 ,  C07K16/28 ,  C07K16/3069 ,  C07K2317/34 ,  C07K2319/00 ,  Y10T436/143333

摘要： Described is a novel family of cell surface serpentine transmembrane antigens. Two of the proteins in this family are exclusively or predominantly expressed in the prostate, as well as in prostate cancer, and thus members of this family have been termed “STRAP” (Serpentine TRansmembrane Antigens of the Prostate). Four particular human STRAPs are described and characterized herein. The human STRAPs exhibit a high degree of structural conservation among them but show no significant structural homology to any known human proteins. The prototype member of the STRAP family, STRAP-1, appears to be a type IIIa membrane protein expressed predominantly in prostate cells in normal human tissues. Structurally, STRAP-1 is a 339 amino acid protein characterized by a molecular topology of six transmembrane domains and intracellular N- and C-termini, suggesting that it folds in a “serpentine” manner into three extracellular and two intracellular loops. STRAP-1 protein expression is maintained at high levels across various stages of prostate cancer. Moreover, STRAP-1 is highly over-expressed in certain other human cancers.

摘要翻译： 描述了一种新型的细胞表面蛇纹石跨膜抗原家族。 这个家族中的两个蛋白质完全或主要地在前列腺中表达，也是在前列腺癌中，因此这个家族的成员被称为“前列腺的蛇纹石逆转膜抗原”。 本文描述和表征了四种特定的人类STRAP。 人类STRAP在其中表现出高度的结构保守性，但与任何已知的人类蛋白质没有显着的结构同源性。 STRAP家族STRAP-1的原型成员似乎是主要在正常人体组织中的前列腺细胞中表达的IIIa型膜蛋白。 在结构上，STRAP-1是一种339个氨基酸的蛋白质，其特征在于六个跨膜结构域和细胞内N-和C-末端的分子拓扑结构，表明它以“蛇纹石”的方式折叠成三个胞外和两个细胞内环。 STRAP-1蛋白表达在前列腺癌的各个阶段保持在高水平。 此外，STRAP-1在某些其他人类癌症中高度过度表达。

公开(公告)号：US20050064445A1

公开(公告)日：2005-03-24

申请号：US10750262

申请日：2003-12-31

申请人： Daniel Afar ,  Rene Hubert ,  Kahan Leong ,  Arthur Raitano ,  Douglas Saffran ,  Stephen Mitchell

发明人： Daniel Afar ,  Rene Hubert ,  Kahan Leong ,  Arthur Raitano ,  Douglas Saffran ,  Stephen Mitchell

IPC分类号： G01N33/574 ,  A61K38/00 ,  A61K39/395 ,  A61P35/00 ,  C07K14/47 ,  C07K14/705 ,  C07K16/18 ,  C12N5/10 ,  C12N15/09 ,  C12P21/00 ,  C12P21/08 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C07K14/4748 ,  C07K14/705 ,  C07K14/723 ,  C07K16/28 ,  C07K16/3069 ,  C07K2317/34 ,  C07K2319/00 ,  Y10T436/143333

摘要： Described is a novel family of cell surface serpentine transmembrane antigens. Two of the proteins in this family are exclusively or predominantly expressed in the prostate, as well as in prostate cancer, and thus members of this family have been termed “STRAP” (Serpentine TRansmembrane Antigens of the Prostate). Four particular human STRAPs are described and characterized herein. The human STRAPs exhibit a high degree of structural conservation among them but show no significant structural homology to any known human proteins. The prototype member of the STRAP family, STRAP-1, appears to be a type IIIa membrane protein expressed predominantly in prostate cells in normal human tissues. Structurally, STRAP-1 is a 339 amino acid protein characterized by a molecular topology of six transmembrane domains and intracellular N- and C-termini, suggesting that It folds In a “serpentine” manner Into three extracellular and two Intracellular loops. STRAP-1 protein expression Is maintained at high levels across various stages of prostate cancer. Moreover, STRAP-1 is highly over-expressed in certain other human cancers.

摘要翻译： 描述了一种新型的细胞表面蛇纹石跨膜抗原家族。 这个家族中的两个蛋白质完全或主要地在前列腺中表达，也是在前列腺癌中，因此这个家族的成员被称为“前列腺的蛇纹石逆转膜抗原”。 本文描述和表征了四种特定的人类STRAP。 人类STRAP在其中表现出高度的结构保守性，但与任何已知的人类蛋白质没有显着的结构同源性。 STRAP家族STRAP-1的原型成员似乎是主要在正常人体组织中的前列腺细胞中表达的IIIa型膜蛋白。 结构上，STRAP-1是一种339个氨基酸的蛋白质，其特征在于六个跨膜结构域和细胞内N-和C-末端的分子拓扑结构，表明它以“蛇纹石”的方式折叠成三个细胞外和两个细胞内环。 STRAP-1蛋白表达在前列腺癌的各个阶段保持在高水平。 此外，STRAP-1在某些其他人类癌症中高度过度表达。

公开(公告)号：US20050019870A1

公开(公告)日：2005-01-27

申请号：US10936138

申请日：2004-09-07

申请人： Daniel Afar ,  Rene Hubert ,  Kahan Leong ,  Arthur Raitano ,  Douglas Saffran

发明人： Daniel Afar ,  Rene Hubert ,  Kahan Leong ,  Arthur Raitano ,  Douglas Saffran

IPC分类号： A61K38/17 ,  C07K14/705 ,  G01N33/574 ,  A61K39/395 ,  C07H21/04

CPC分类号： C07K14/705 ,  A01K2217/05 ,  A61K38/1709 ,  C12N2799/026 ,  C12N2799/027 ,  G01N33/57492

摘要： A novel prostate tumor associated gene (designated 24P4C12) and its encoded protein is described. 24P4C12 is highly expressed in prostate tissue xenografts, providing evidence that it is turned on in at least some prostate cancers. 24P4C12 provides a diagnostic and/or therapeutic target for prostate and other cancers.

摘要翻译： 描述了一种新型前列腺肿瘤相关基因（称为24P4C12）及其编码的蛋白质。 24P4C12在前列腺组织异种移植物中高度表达，提供证据表明它在至少一些前列腺癌中被开启。 24P4C12提供了前列腺癌和其他癌症的诊断和/或治疗靶点。

公开(公告)号：US20060147951A1

公开(公告)日：2006-07-06

申请号：US11225661

申请日：2005-09-12

申请人： Daniel Afar ,  Rene Hubert ,  Kahan Leong ,  Arthur Raitano ,  Douglas Saffran ,  Stephen Mitchell

发明人： Daniel Afar ,  Rene Hubert ,  Kahan Leong ,  Arthur Raitano ,  Douglas Saffran ,  Stephen Mitchell

IPC分类号： C12Q1/68 ,  G01N33/574 ,  C07H21/04 ,  C12P21/06 ,  C07K14/82 ,  C07K16/30

CPC分类号： C07K14/4748 ,  C07K14/705 ,  C07K14/723 ,  C07K16/28 ,  C07K16/3069 ,  C07K2317/34 ,  C07K2319/00 ,  Y10T436/143333

摘要： Described is a novel family of cell surface serpentine transmembrane antigens. Two of the proteins in this family are exclusively or predominantly expressed in the prostate, as well as in prostate cancer, and thus members of this family have been termed “STRAP” Serpentine TRansmembrane Antigens of the Prostate). Four particular human STRAPs are described and characterized herein. The human STRAPs exhibit a high degree of structural conservation among them but show no significant structural homology to any known human proteins. The prototype member of the STRAP family, STRAP-1, appears to be a type IIIa membrane protein expressed predominantly in prostate cells in normal human tissues. Structurally, STRAP-1 is a 339 amino acid protein characterized by a molecular topology of six transmembrane domains and Intracellular N- and C-termini, suggesting that It folds In a “serpentine” manner Into three extracellular and two intracellular loops. STRAP-1 protein expression is maintained at high levels across various stages of prostate cancer. Moreover, STRAP-1 is highly over-expressed In certain other human cancers.

摘要翻译： 描述了一种新型的细胞表面蛇纹石跨膜抗原家族。 这个家族中的两个蛋白质完全或主要地在前列腺中表达，以及在前列腺癌中，因此该家族的成员被称为前列腺的“STRAP”蛇纹石TRANmembrane抗原。 本文描述和表征了四种特定的人类STRAP。 人类STRAP在其中表现出高度的结构保守性，但与任何已知的人类蛋白质没有显着的结构同源性。 STRAP家族STRAP-1的原型成员似乎是主要在正常人体组织中的前列腺细胞中表达的IIIa型膜蛋白。 在结构上，STRAP-1是一种339个氨基酸的蛋白质，其特征在于六个跨膜结构域和细胞内N-和C-末端的分子拓扑结构，表明它以“蛇纹石”方式折叠成三个细胞外和两个细胞内环。 STRAP-1蛋白表达在前列腺癌的各个阶段保持在高水平。 此外，STRAP-1在某些其他人类癌症中高度过度表达。

公开(公告)号：US07037667B1

公开(公告)日：2006-05-02

申请号：US09615285

申请日：2000-07-12

申请人： Daniel E. H. Afar ,  Rene S. Hubert ,  Kahan Leong ,  Arthur B. Raitano ,  Douglas Saffran ,  Stephen C. Mitchell ,  Aya Jakobovits ,  Mary Faris ,  Igor Vivanco

发明人： Daniel E. H. Afar ,  Rene S. Hubert ,  Kahan Leong ,  Arthur B. Raitano ,  Douglas Saffran ,  Stephen C. Mitchell ,  Aya Jakobovits ,  Mary Faris ,  Igor Vivanco

IPC分类号： G01N33/574 ,  G01N33/48 ,  C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2600/136 ,  C12Q2600/154 ,  G01N33/57419 ,  G01N33/57434 ,  G01N33/57488 ,  G01N2333/95

摘要： Compositions for the diagnosis and therapy of prostate and colon cancer, derived from or based on a novel prostate-specific, androgen-related, cell membrane associated and secreted serine protease termed 20P1F12/TMPRSS2 are described. A full length cDNA comprising the entire coding sequence of the 20P1F12/TMPRSS2 gene (also designated 20P1F12-GTC1 herein) is provided (FIG. 1). Among the compositions provided are antibodies that bind to 20P1F12/TMPRSS2 proteins and polypeptide fragments thereof, including antibodies labeled with a detectable marker or toxin or therapeutic composition. The invention also provides prognostic and diagnostic methods of examining a biological sample for evidence of disregulated cellular growth by comparing the status of 20P1F12/TMPRSS2 in the biological sample to the status of 20P1F12/TMPRSS2 in a corresponding normal sample, wherein alterations in the status of 20P1F12/TMPRSS2 in the biological sample are associated with disregulated cellular growth. The invention further provides various therapeutic compositions and strategies for treating prostate cancer, including particularly, 20P1F12/TMPRSS2 polypeptide and anti-20P1F12/TMPRSS2 antibody therapy methods and compositions, cancer vaccines, and small molecule therapy.

摘要翻译： 描述了源自或基于名为20P1F12 / TMPRSS2的新型前列腺特异性雄激素相关的细胞膜相关和分泌的丝氨酸蛋白酶的前列腺和结肠癌的诊断和治疗的组合物。 提供了包含20P1F12 / TMPRSS2基因（本文中也称为20P1F12-GTC1）的整个编码序列的全长cDNA（图1）。 所提供的组合物是结合20P1F12 / TMPRSS2蛋白和其多肽片段的抗体，包括用可检测标记或毒素或治疗组合物标记的抗体。 本发明还提供了通过将生物样品中20P1F12 / TMPRSS2的状态与对应的正常样品中的20P1F12 / TMPRSS2的状态进行比较来检查生物样品以检测细胞生长失调的证据的预后和诊断方法，其中， 生物样品中的20P1F12 / TMPRSS2与细胞生长失调有关。 本发明还提供了治疗前列腺癌的各种治疗组合物和策略，特别包括20P1F12 / TMPRSS2多肽和抗-20P1F12 / TMPRSS2抗体治疗方法和组合物，癌症疫苗和小分子治疗。

公开(公告)号：US07981624B2

公开(公告)日：2011-07-19

申请号：US12389293

申请日：2009-02-19

申请人： Daniel E. H. Afar ,  Rene S. Hubert ,  Kahan Leong ,  Arthur B. Raitano ,  Douglas Saffran ,  Stephen C. Mitchell ,  Aya Jakobovits ,  Mary Faris ,  Igor Vivanco

发明人： Daniel E. H. Afar ,  Rene S. Hubert ,  Kahan Leong ,  Arthur B. Raitano ,  Douglas Saffran ,  Stephen C. Mitchell ,  Aya Jakobovits ,  Mary Faris ,  Igor Vivanco

IPC分类号： G01N33/53

CPC分类号： C12Q1/6886 ,  C12Q2600/136 ,  C12Q2600/154 ,  G01N33/57419 ,  G01N33/57434 ,  G01N33/57488 ,  G01N2333/95

摘要： Compositions for the diagnosis and therapy of prostate and colon cancer, derived from or based on a novel prostate-specific, androgen-regulated, cell membrane associated and secreted serine protease termed 20P1F12/TMPRSS2 are described. A full length cDNA comprising the entire coding sequence of the 20P1F12/TMPRSS2 gene (also designated 20P1F12-GTC1 herein) is provided (FIG. 1). Among the compositions provided are antibodies that bind to 20P1F12/TMPRSS2 proteins and polypeptide fragments thereof, including antibodies labeled with a detectable marker or toxin or therapeutic composition. The invention also provides prognostic and diagnostic methods of examining a biological sample for evidence of disregulated cellular growth by comparing the status of 20P1F12/TMPRSS2 in the biological sample to the status of 20P1F12/TMPRSS2 in a corresponding normal sample, wherein alterations in the status of 20P1F12/TMPRSS2 in the biological sample are associated with disregulated cellular growth. The invention further provides various therapeutic compositions and strategies for treating prostate cancer, including particularly, 20P1F12/TMPRSS2 polypeptide and anti-20P1F12/TMPRSS2 antibody therapy methods and compositions, cancer vaccines, and small molecule therapy.

摘要翻译： 描述了衍生自或基于称为20P1F12 / TMPRSS2的新型前列腺特异性，雄激素调节的细胞膜相关和分泌的丝氨酸蛋白酶的前列腺和结肠癌的诊断和治疗的组合物。 提供了包含20P1F12 / TMPRSS2基因（本文中也称为20P1F12-GTC1）的整个编码序列的全长cDNA（图1）。 所提供的组合物是结合20P1F12 / TMPRSS2蛋白和其多肽片段的抗体，包括用可检测标记或毒素或治疗组合物标记的抗体。 本发明还提供了通过将生物样品中20P1F12 / TMPRSS2的状态与对应的正常样品中的20P1F12 / TMPRSS2的状态进行比较来检查生物样品以检测细胞生长失调的证据的预后和诊断方法，其中， 生物样品中的20P1F12 / TMPRSS2与细胞生长失调有关。 本发明还提供了治疗前列腺癌的各种治疗组合物和策略，特别包括20P1F12 / TMPRSS2多肽和抗-20P1F12 / TMPRSS2抗体治疗方法和组合物，癌症疫苗和小分子治疗。