公开(公告)号：US06713257B2

公开(公告)日：2004-03-30

申请号：US09781814

申请日：2001-02-12

申请人： Daniel D. Shoemaker ,  Stewart Scherer ,  Steven J. Altschuler ,  Lani F. Wu ,  Christopher D. Armour

发明人： Daniel D. Shoemaker ,  Stewart Scherer ,  Steven J. Altschuler ,  Lani F. Wu ,  Christopher D. Armour

IPC分类号： C12Q168

CPC分类号： G06F19/18 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00743 ,  C12Q1/6809 ,  C12Q1/6837 ,  C40B40/06 ,  G06F19/20

摘要： The invention relates to methods and systems (e.g., computer systems and computer program products) for identifying and characterizing genes using microarrays. In particular, the invention provides for improved, robust methods for detecting genes through the use of microarrays to analyze the expression state of the genome. Genes which are expressed can be mapped to their respective positions in the genome, and the structure of such genes can be determined.

公开(公告)号：US07432084B2

公开(公告)日：2008-10-07

申请号：US10485008

申请日：2002-07-17

申请人： Daniel D. Shoemaker ,  Christopher D. Armour ,  Philip W. Garrett-Engele

发明人： Daniel D. Shoemaker ,  Christopher D. Armour ,  Philip W. Garrett-Engele

IPC分类号： C12P19/34

CPC分类号： C12N15/1096 ,  C12Q1/6837 ,  C12Q1/6865 ,  C12Q2563/107

摘要： In one aspect the present invention provides methods of synthesizing a preparation of nucleic acid molecules, the methods comprising the steps of: (a) utilizing an RNA template to enzymatically synthesize a first DNA molecule that is complementary to at least 50 contiguous bases of the RNA template; (b) utilizing the first DNA molecule as a template to enzymatically synthesize a second DNA molecule, thereby forming a double-stranded DNA molecule wherein the first DNA molecule is hybridized to the second DNA molecule; (c) utilizing the first or second DNA molecule of the double-stranded DNA molecule as a template to enzymatically synthesize a first RNA molecule that is complementary to either the first DNA molecule or to the second DNA molecule; and (d) utilizing the first RNA molecule as a template to enzymatically synthesize a third DNA molecule that is complementary to the first RNA molecule. In another aspect, the present invention provides processed DNA samples prepared by a method of the invention for synthesizing a preparation of nucleic acid molecules. In another aspect, the present invention provides methods for hybridizing a processed DNA sample to a population of immobilized nucleic acid molecules.

摘要翻译： 一方面，本发明提供了合成核酸分子制剂的方法，所述方法包括以下步骤：（a）利用RNA模板来酶促合成与RNA的至少50个连续碱基互补的第一DNA分子 模板; （b）利用第一DNA分子作为模板来酶促合成第二DNA分子，从而形成双链DNA分子，其中第一DNA分子与第二DNA分子杂交; （c）利用双链DNA分子的第一或第二DNA分子作为模板来酶促合成与第一DNA分子或第二DNA分子互补的第一RNA分子; 和（d）利用第一RNA分子作为模板来酶促合成与第一RNA分子互补的第三DNA分子。 另一方面，本发明提供了通过本发明的用于合成核酸分子制剂的方法制备的加工DNA样品。 另一方面，本发明提供了将经处理的DNA样品与固定的核酸分子群杂交的方法。

公开(公告)号：US07150969B2

公开(公告)日：2006-12-19

申请号：US11144368

申请日：2005-06-03

申请人： Zhengyan Kan ,  Philip W. Garrett-Engele ,  Christopher D. Armour ,  Christopher K. Raymond ,  John C. Castle

发明人： Zhengyan Kan ,  Philip W. Garrett-Engele ,  Christopher D. Armour ,  Christopher K. Raymond ,  John C. Castle

IPC分类号： C12Q1/48 ,  C12Q1/68 ,  C12N15/70 ,  C12N9/10 ,  C12N5/16 ,  C07H21/04

CPC分类号： C12N9/93

摘要： The present invention features nucleic acids and polypeptides encoding novel splice variant isoforms of acetyl-CoA carboxylase 2 (ACC2). The polynucleotide sequence of ACC2sv1 is provided by SEQ ID NO 3. The amino acid sequence of ACC2sv1 is provided by SEQ ID NO 4. The present invention also provides methods for using ACC2sv1 polynucleotides and proteins to screen for compounds that bind to ACC2sv1.

摘要翻译： 本发明的特征在于编码乙酰辅酶A羧化酶2（ACC2）的剪接变异体亚型的核酸和多肽。 ACC2sv1的多核苷酸序列由SEQ ID NO 3提供。ACC2sv1的氨基酸序列由SEQ ID NO 4提供。本发明还提供了使用ACC2sv1多核苷酸和蛋白质筛选结合ACC2sv1的化合物的方法。