利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

20 条记录 (耗时 0.021 秒)

    Nucleic acid sequence amplification method, composition and kit
    3.
    发明授权
    Nucleic acid sequence amplification method, composition and kit 失效
    核酸序列扩增法，组成和试剂盒

    公开(公告)号：US5554516A

    公开(公告)日：1996-09-10

    申请号：US162836

    申请日：1993-12-02

    申请人： Daniel L. Kacian ,  Diane L. McAllister ,  Sherrol H. McDonough ,  Nanibhushan Dattagupta

    发明人： Daniel L. Kacian ,  Diane L. McAllister ,  Sherrol H. McDonough ,  Nanibhushan Dattagupta

    IPC分类号： C12N15/09 ,  C07H21/00 ,  C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C07H21/00 ,  C12Q1/6848 ,  C12Q1/686 ,  C12Q1/6865 ,  C12Q1/689

    摘要： A method, composition and kit for amplifying a target nucleic acid sequence under conditions of substantially constant temperature, ionic strength, and pH and using only a single promoter-primer. To effect the amplification, a supply of a single promoter-primer having a promoter and a primer complementary to the 3'-end of the target sequence, and a reverse transcriptase and an RNA polymerase are provided to a mixture including the target sequence; the amplification proceeds accordingly. The invention is useful for generating copies of a nucleic acid target sequence for purposes that include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.

    摘要翻译： 用于在基本恒定的温度，离子强度和pH条件下扩增靶核酸序列的方法，组合物和试剂盒，并且仅使用单个启动子引物。 为了进行扩增，向包含目标序列的混合物提供具有启动子和与靶序列的3'末端互补的引物的单个启动子 - 引物供应源和逆转录酶和RNA聚合酶; 相应地进行放大。 本发明可用于产生核酸靶序列的拷贝，目的包括在临床，环境，法医学和相似样品中定量特异性核酸序列的测定，克隆和产生探针。

    Oligonucleotide probes and methods for detecting Streptococcus pneumoniae
    6.
    发明授权
    Oligonucleotide probes and methods for detecting Streptococcus pneumoniae 失效
    寡核苷酸探针和检测肺炎链球菌的方法

    公开(公告)号：US5888729A

    公开(公告)日：1999-03-30

    申请号：US486915

    申请日：1995-06-07

    申请人： Daniel L. Kacian ,  Diane L. McAllister ,  Sherrol H. McDonough ,  Nanibhushan Dattagupta

    发明人： Daniel L. Kacian ,  Diane L. McAllister ,  Sherrol H. McDonough ,  Nanibhushan Dattagupta

    IPC分类号： C12N15/09 ,  C07H21/00 ,  C12Q1/68 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C07H21/00 ,  C12Q1/6848 ,  C12Q1/686 ,  C12Q1/6865 ,  C12Q1/689

    摘要： A method, composition and kit for amplifying a target nucleic acid sequence under conditions of substantially constant temperature, ionic strength, and pH and using only a single promoter-primer. To effect the amplification, a supply of a single promoter-primer having a promoter and a primer complementary to the 3'-end of the target sequence, and a reverse transcriptase and an RNA polymerase are provided to a mixture including the target sequence; the amplification proceeds accordingly. The invention is useful for generating copies of a nucleic acid target sequence for purposes that include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.

    摘要翻译： 用于在基本恒定的温度，离子强度和pH条件下扩增靶核酸序列的方法，组合物和试剂盒，并且仅使用单个启动子引物。 为了进行扩增，向包含目标序列的混合物提供具有启动子和与靶序列的3'末端互补的引物的单个启动子 - 引物供应源和逆转录酶和RNA聚合酶; 相应地进行放大。 本发明可用于产生核酸靶序列的拷贝，目的包括在临床，环境，法医学和相似样品中定量特异性核酸序列的测定，克隆和产生探针。

    Protection probes
    8.
    发明授权
    Protection probes 有权
    保护探头

    公开(公告)号：US06245519B1

    公开(公告)日：2001-06-12

    申请号：US09502966

    申请日：2000-02-11

    申请人： Steven T. Brentano ,  Sherrol H. McDonough ,  Norman C. Nelson

    发明人： Steven T. Brentano ,  Sherrol H. McDonough ,  Norman C. Nelson

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6813 ,  C12Q1/6806 ,  C12Q1/6832 ,  C12Q2527/125 ,  C12Q2527/107 ,  C12Q2525/117 ,  C12Q2563/103 ,  C12Q2561/108 ,  C12Q2537/113 ,  C12Q2537/162

    摘要： The present invention features compositions and methods that are useful for storing labeled detection probes and detecting whether a target nucleic acid sequence is present in a sample. Preferred compositions are made up of a detection probe containing a label susceptible to a chemical or enzymatic alteration and a protection probe that protects the label from alteration and/or decreases the ability of the detection probe to inhibit nucleic acid amplification. Such compositions can be used, for example, to stabilize a detection probe label and to prevent a detection probe from hybridizing prematurely to amplified or target nucleic acid.

    摘要翻译： 本发明的特征在于可用于存储标记的检测探针并检测样品中是否存在靶核酸序列的组合物和方法。 优选的组合物由含有对化学或酶促改变敏感的标记的检测探针和保护标记免受改变和/或降低检测探针抑制核酸扩增能力的保护探针组成。 这样的组合物可以用于例如稳定检测探针标记并防止检测探针过早与扩增或靶核酸杂交。