Nucleic acid sequence amplification methods
    5.
    发明授权
    Nucleic acid sequence amplification methods 失效
    核酸序列扩增方法

    公开(公告)号:US5399491A

    公开(公告)日:1995-03-21

    申请号:US855732

    申请日:1992-03-19

    CPC分类号: C12Q1/6855 Y10S435/975

    摘要: Methods of synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies. These methods are useful for generating copies of a nucleic acid target sequence for purposes which include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.

    摘要翻译: 提供了在基本恒定的温度,离子强度和pH条件下自动催化靶核酸序列的多个拷贝的方法,其中靶序列的多个RNA拷贝自动催化产生另外的拷贝。 这些方法可用于产生核酸靶序列的拷贝,目的包括在临床,环境,法医和相似样品中定量特异性核酸序列的测定,克隆和产生探针。