公开(公告)号：US20090263813A1

公开(公告)日：2009-10-22

申请号：US12421188

申请日：2009-04-09

申请人： DAVID H GELFAND ,  Ivo Glynne Gut ,  Keith A. Bauer ,  Florence Mauger

发明人： DAVID H GELFAND ,  Ivo Glynne Gut ,  Keith A. Bauer ,  Florence Mauger

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6858 ,  C12Q1/686

摘要： The present application provides polynucleotides comprising 5′-tails with sequence segments useful for the detection of target nucleic acid sequences, and methods for their use in detecting target nucleic acids. The polynucleotides are used to amplify a subsequence of a target nucleic acid in the presence of one or more ribonucleotides. The ribonucleotides are incorporated into amplification products at regular intervals complementary to the 5′-tail sequence segments. Cleavage of amplification products at the bond immediately 3′ to incorporated ribonucleotides produces detectably distinct fragments indicative of the presence or absence of a target nucleic acid.

摘要翻译： 本申请提供了包含具有用于检测靶核酸序列的序列区段的5'-尾的多核苷酸，以及它们用于检测靶核酸的方法。 多核苷酸用于在一个或多个核糖核苷酸存在下扩增靶核酸的亚序列。 核糖核苷酸以与5'-尾序列片段互补的规则间隔掺入扩增产物中。 立即将3'端的扩增产物切割成掺入的核糖核苷酸产生指示靶核酸存在或不存在的可检测的不同片段。

公开(公告)号：US10150990B2

公开(公告)日：2018-12-11

申请号：US12421188

申请日：2009-04-09

申请人： David H. Gelfand ,  Ivo Glynne Gut ,  Keith A. Bauer ,  Florence Mauger

发明人： David H. Gelfand ,  Ivo Glynne Gut ,  Keith A. Bauer ,  Florence Mauger

IPC分类号： C12P1/68 ,  C12P19/34 ,  C12N9/00 ,  G01N31/00 ,  G01N33/00 ,  C07H21/04 ,  C07H21/00 ,  C12Q1/6858 ,  C12Q1/686

摘要： The present application provides polynucleotides comprising 5′-tails with sequence segments useful for the detection of target nucleic acid sequences, and methods for their use in detecting target nucleic acids. The polynucleotides are used to amplify a subsequence of a target nucleic acid in the presence of one or more ribonucleotides. The ribonucleotides are incorporated into amplification products at regular intervals complementary to the 5′-tail sequence segments. Cleavage of amplification products at the bond immediately 3′ to incorporated ribonucleotides produces detectably distinct fragments indicative of the presence or absence of a target nucleic acid.

公开(公告)号：US20060172306A1

公开(公告)日：2006-08-03

申请号：US10563090

申请日：2004-07-02

申请人： Glynne Ivo Gut ,  Florence Mauger

发明人： Glynne Ivo Gut ,  Florence Mauger

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6827 ,  C12Q1/6872 ,  C12Q2565/627 ,  C12Q2525/101 ,  C12Q2523/319

摘要： A method for the detection in a given DNA sequence of DNA mutations, single nucleotide polymorphisms, and insertions and deletions, said method comprising the step of a) producing replicate(s) with an engineered polymerase of said given DNA sequence with part or all of at least one of the four natural DNA bases exchanged against a not natural base; b) using said not natural base to cleave the replicate(s) obtained in step a) and to produce a DNA product presenting sequence-specific fragments; c) analyzing said sequence-specific fragments obtained in step b) by mass spectrometry to get sequence-specific fragment patterns; and d) using the sequence-specific fragment patterns obtained in step c) to identify sequence changes relative to a reference to said given DNA sequence. A kit for the detection of DNA mutations, single nucleotide polymorphisms, and insertions and deletions.

摘要翻译： 一种用于在给定DNA序列中检测DNA突变，单核苷酸多态性以及插入和缺失的方法，所述方法包括以下步骤：a）用所述给定DNA序列的工程聚合酶产生重复的一部分或全部 四种天然DNA碱基中的至少一种与非天然碱基交换; b）使用所述非天然碱切割步骤a）中获得的复制物并产生呈现序列特异性片段的DNA产物; c）通过质谱分析步骤b）中获得的序列特异性片段以获得序列特异性片段模式; 和d）使用步骤c）中获得的序列特异性片段模式来鉴定相对于所述给定DNA序列的参考的序列变化。 用于检测DNA突变，单核苷酸多态性以及插入和缺失的试剂盒。