公开(公告)号：US06344316B1

公开(公告)日：2002-02-05

申请号：US08882649

申请日：1997-06-25

申请人： David J. Lockhart ,  Mark Chee ,  Kevin Gunderson ,  Lai Chaoqiang ,  Lisa Wodicka ,  Maureen T. Cronin ,  Danny Lee ,  Huu M. Tran ,  Hajime Matsuzaki

发明人： David J. Lockhart ,  Mark Chee ,  Kevin Gunderson ,  Lai Chaoqiang ,  Lisa Wodicka ,  Maureen T. Cronin ,  Danny Lee ,  Huu M. Tran ,  Hajime Matsuzaki

IPC分类号： C12Q168

CPC分类号： C07H19/052 ,  C07H19/12 ,  C07H21/00 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q2600/156 ,  C40B40/00 ,  G06F19/20 ,  G06F19/22 ,  C12Q2525/161 ,  C12Q2565/501 ,  C12Q2561/125

摘要： The present invention provides a simplified method for identifying differences in nucleic acid abundances (e.g., expression levels) between two or more samples. The methods involve providing an array containing a large number (e.g. greater than 1,000) of arbitrarily selected different oligonucleotide probes where the sequence and location of each different probe is known. Nucleic acid samples (e.g. mRNA) from two or more samples are hybridized to the probe arrays and the pattern of hybridization is detected. Differences in the hybridization patterns between the samples indicates differences in expression of various genes between those samples. This invention also provides a method of end-labeling a nucleic acid. In one embodiment, the method involves providing a nucleic acid, providing a labeled oligonucleotide and then enzymatically ligating the oligonucleotide to the nucleic acid. Thus, for example, where the nucleic acid is an RNA, a labeled oligoribonucleotide can be ligated using an RNA ligase. In another embodiment, the end labeling can be accomplished by providing a nucleic acid, providing labeled nucleoside triphosphates, and attaching the nucleoside triphosphates to the nucleic acid using a terminal transferase.

摘要翻译： 本发明提供用于鉴定两个或多个样品之间的核酸丰度差异（例如，表达水平）的简化方法。 所述方法包括提供包含大量（例如大于1,000个）任意选择的不同寡核苷酸探针的阵列，其中每个不同探针的序列和位置是已知的。 来自两个或更多个样品的核酸样品（例如mRNA）与探针阵列杂交，并检测杂交模式。 样本之间的杂交模式的差异表明这些样品之间各种基因的表达差异。 本发明还提供了一种终止标记核酸的方法。 在一个实施方案中，该方法包括提供核酸，提供标记的寡核苷酸，然后将寡核苷酸酶连接到核酸上。 因此，例如，当核酸是RNA时，可以使用RNA连接酶连接标记的寡核糖核苷酸。 在另一个实施方案中，末端标记可以通过提供核酸，提供标记的核苷三磷酸和使用末端转移酶将核苷三磷酸与核酸连接来实现。

公开(公告)号：US08398839B1

公开(公告)日：2013-03-19

申请号：US12793370

申请日：2010-06-03

申请人： Alfredo M. Morales ,  Josh A. Whaley ,  Mark D. Zimmerman ,  Ronald F. Renzi ,  Huu M. Tran ,  Scott M. Maurer ,  William D. Munslow

发明人： Alfredo M. Morales ,  Josh A. Whaley ,  Mark D. Zimmerman ,  Ronald F. Renzi ,  Huu M. Tran ,  Scott M. Maurer ,  William D. Munslow

IPC分类号： B03C5/02

CPC分类号： B03C5/005 ,  B03C5/024 ,  B03C5/026

摘要： A new microfluidic system comprising an automated prototype insulator-based dielectrophoresis (iDEP) triggering microfluidic device for pathogen monitoring that can eventually be run outside the laboratory in a real world environment has been used to demonstrate the feasibility of automated trapping and detection of particles. The system broadly comprised an aerosol collector for collecting air-borne particles, an iDEP chip within which to temporarily trap the collected particles and a laser and fluorescence detector with which to induce a fluorescence signal and detect a change in that signal as particles are trapped within the iDEP chip.

摘要翻译： 一种新的微流体系统已经被用于证明自动捕获和检测颗粒的可行性，该自动原型基于绝缘子的介电电泳（iDEP）触发用于病原体监测的微流体装置，其可以在实际环境中最终在实验室外运行。 该系统广泛地包括用于收集空气颗粒的气溶胶收集器，其中临时捕获收集的颗粒的iDEP芯片和用于诱导荧光信号的激光和荧光检测器，并且当颗粒被捕获在其内时，检测该信号的变化 iDEP芯片。

公开(公告)号：US5527680A

公开(公告)日：1996-06-18

申请号：US404687

申请日：1995-03-15

申请人： Huu M. Tran ,  Diana M. Smith ,  Lois B. Epstein

发明人： Huu M. Tran ,  Diana M. Smith ,  Lois B. Epstein

IPC分类号： G01N27/447 ,  C12Q1/00 ,  B01D57/02 ,  B01D61/42 ,  C25B7/00

CPC分类号： G01N27/44717

摘要： A flow-through receptacle is disclosed, one end of which is designed to hold multiple plugs of gel material, containing solutes such as macromolecules, excised from electrophoretic separations, and the other end of which is capable of insertion into a recipient matrix that lies between the plates of a slab gel enclosure in an electrophoresis apparatus. The receptacle is used to place the gel plugs, containing the macromolecules or solutes, into electrophoretic contact with a recipient matrix and allows for electrophoretic transfer of the solutes from the plugs into a single concentrated zone in the recipient matrix. The concentrated macromolecules or solutes can then be further processed in the recipient gel or excised and used for procedures requiring greater amounts and concentrations of the solute than are available in the original plugs.

摘要翻译： 公开了一种流通容器，其一端被设计成容纳凝胶材料的多个塞子，其包含从电泳分离物切下的溶质如大分子，并且其另一端能够插入位于 电泳装置中的平板凝胶封套的板。 容器用于将含有大分子或溶质的凝胶塞与受体基质电泳接触，并允许将溶质从栓塞电泳转移到受体基质中的单个浓缩区域中。 然后可以将浓缩的大分子或溶质在接收器凝胶中进一步处理或切除，并用于需要比原始插塞中可获得更大量和浓度的溶质的步骤。