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公开(公告)号：US08398839B1
公开(公告)日：2013-03-19
申请号：US12793370
申请日：2010-06-03
申请人： Alfredo M. Morales , Josh A. Whaley , Mark D. Zimmerman , Ronald F. Renzi , Huu M. Tran , Scott M. Maurer , William D. Munslow
发明人： Alfredo M. Morales , Josh A. Whaley , Mark D. Zimmerman , Ronald F. Renzi , Huu M. Tran , Scott M. Maurer , William D. Munslow
IPC分类号： B03C5/02
摘要： A new microfluidic system comprising an automated prototype insulator-based dielectrophoresis (iDEP) triggering microfluidic device for pathogen monitoring that can eventually be run outside the laboratory in a real world environment has been used to demonstrate the feasibility of automated trapping and detection of particles. The system broadly comprised an aerosol collector for collecting air-borne particles, an iDEP chip within which to temporarily trap the collected particles and a laser and fluorescence detector with which to induce a fluorescence signal and detect a change in that signal as particles are trapped within the iDEP chip.
摘要翻译： 一种新的微流体系统已经被用于证明自动捕获和检测颗粒的可行性，该自动原型基于绝缘子的介电电泳（iDEP）触发用于病原体监测的微流体装置，其可以在实际环境中最终在实验室外运行。 该系统广泛地包括用于收集空气颗粒的气溶胶收集器，其中临时捕获收集的颗粒的iDEP芯片和用于诱导荧光信号的激光和荧光检测器，并且当颗粒被捕获在其内时，检测该信号的变化 iDEP芯片。
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公开(公告)号：US08828736B2
公开(公告)日：2014-09-09
申请号：US13173180
申请日：2011-06-30
CPC分类号： B01L3/502761 , B01L2200/0668 , B01L2300/0645 , B01L2300/0864 , C12M35/02 , Y10T436/2575
摘要： We have developed an microelectroporation device that combines microarrays of oligonucleotides, microfluidic channels, and electroporation for cell transfection and high-throughput screening applications (e.g. RNA interference screens). Microarrays allow the deposition of thousands of different oligonucleotides in microscopic spots. Microfluidic channels and microwells enable efficient loading of cells into the device and prevent cross-contamination between different oligonucleotides spots. Electroporation allows optimal transfection of nucleic acids into cells (especially hard-to-transfect cells such as primary cells) by minimizing cell death while maximizing transfection efficiency. This invention has the advantage of a higher throughput and lower cost, while preventing cross-contamination compared to conventional screening technologies. Moreover, this device does not require bulky robotic liquid handling equipment and is inherently safer given that it is a closed system.
摘要翻译： 我们开发了一种微电极装置，其结合寡核苷酸微阵列，微流体通道和电穿孔用于细胞转染和高通量筛选应用（例如RNA干扰筛选）。 微阵列允许在微观斑点中沉积数千种不同的寡核苷酸。 微流体通道和微孔能够有效地将细胞装载到装置中并防止不同寡核苷酸斑点之间的交叉污染。 电穿孔允许通过最大限度地减少细胞死亡同时使转染效率最大化将核酸最佳地转染到细胞（特别是难转染细胞，如原代细胞）中。 本发明具有较高的生产能力和较低的成本，同时防止与常规筛选技术相比的交叉污染。 此外，该装置不需要庞大的机器人液体处理设备，并且由于它是封闭的系统，所以固有地更安全。
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公开(公告)号：US20130020492A1
公开(公告)日：2013-01-24
申请号：US13559370
申请日：2012-07-26
IPC分类号： G01T3/00
摘要： A neutron detector with monolithically integrated readout circuitry, including: a bonded semiconductor die; an ion chamber formed in the bonded semiconductor die; a first electrode and a second electrode formed in the ion chamber; a neutron absorbing material filling the ion chamber; and the readout circuitry which is electrically coupled to the first and second electrodes. The bonded semiconductor die includes an etched semiconductor substrate bonded to an active semiconductor substrate. The readout circuitry is formed in a portion of the active semiconductor substrate. The ion chamber has a substantially planar first surface on which the first electrode is formed and a substantially planar second surface, parallel to the first surface, on which the second electrode is formed. Desirably, the distance between the first electrode and the second electrode may be equal to or less than the 50% attenuation length for neutrons in the neutron absorbing material filling the ion chamber.
摘要翻译： 一种具有单片集成读出电路的中子检测器，包括：键合半导体管芯; 形成在所述键合半导体管芯中的离子室; 形成在所述离子室中的第一电极和第二电极; 填充离子室的中子吸收材料; 以及电耦合到第一和第二电极的读出电路。 键合的半导体管芯包括结合到有源半导体衬底的蚀刻半导体衬底。 读出电路形成在有源半导体衬底的一部分中。 离子室具有基本上平面的第一表面，第一电极形成在其上，并且平行于第一表面的基本平坦的第二表面，在其上形成第二电极。 理想地，第一电极和第二电极之间的距离可以等于或小于填充离子室的中子吸收材料中的中子的50％的衰减长度。
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4.发明授权Microfluidic structures and methods for integrating a functional component into a microfluidic device 有权用于将功能组分整合到微流体装置中的微流体结构和方法公开(公告)号：US07351380B2
公开(公告)日：2008-04-01
申请号：US10754286
申请日：2004-01-08
申请人： Blake Simmons , Linda Domeier , Noble Woo , Timothy Shepodd , Ronald F. Renzi
发明人： Blake Simmons , Linda Domeier , Noble Woo , Timothy Shepodd , Ronald F. Renzi
CPC分类号： F16K99/0001 , B81C3/002 , B81C2201/034 , F15C5/00 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , Y10T436/11 , Y10T436/2575
摘要： Injection molding is used to form microfluidic devices with integrated functional components. One or more functional components are placed in a mold cavity which is then closed. Molten thermoplastic resin is injected into the mold and then cooled, thereby forming a solid substrate including the functional component(s). The solid substrate including the functional component(s) is then bonded to a second substrate which may include microchannels or other features.
摘要翻译： 注射成型用于形成具有集成功能组件的微流体装置。 将一个或多个功能部件放置在模腔中，然后关闭模腔。 将熔融的热塑性树脂注入模具中，然后冷却，从而形成包括功能组分的固体基材。 然后将包括功能组分的固体基质结合到可包括微通道或其它特征的第二基底。
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公开(公告)号：US06998598B2
公开(公告)日：2006-02-14
申请号：US10633794
申请日：2003-08-04
申请人： Brent A. Horn , Ronald F. Renzi
发明人： Brent A. Horn , Ronald F. Renzi
CPC分类号： G01N30/74 , B01L3/5027 , G01N21/645 , G01N2201/024
摘要： A modular optical detector system. The detector system is designed to detect the presence of molecules or molecular species by inducing fluorescence with exciting radiation and detecting the emitted fluorescence. Because the system is capable of accurately detecting and measuring picomolar concentrations it is ideally suited for use with microchemical analysis systems generally and capillary chromatographic systems in particular. By employing a modular design, the detector system provides both the ability to replace various elements of the detector system without requiring extensive realignment or recalibration of the components as well as minimal user interaction with the system. In addition, the modular concept provides for the use and addition of a wide variety of components, including optical elements (lenses and filters), light sources, and detection means, to fit particular needs.
摘要翻译： 模块化光学检测器系统。 探测器系统设计用于通过用激发辐射诱导荧光并检测发射的荧光来检测分子或分子物质的存在。 因为该系统能够精确地检测和测量皮摩尔浓度，所以它理想地适用于通常用于微量化学分析系统和特别是毛细管色谱系统。 通过采用模块化设计，检测器系统提供了替换检测器系统的各种元件的能力，而不需要对组件进行大量的重新对准或重新校准，以及最小化与系统的用户交互。 此外，模块化概念提供了各种组件的使用和添加，包括光学元件（透镜和滤光片），光源和检测装置，以适应特殊需要。
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公开(公告)号：US06926313B1
公开(公告)日：2005-08-09
申请号：US10405204
申请日：2003-04-02
申请人： Ronald F. Renzi
发明人： Ronald F. Renzi
IPC分类号： B01J19/00 , B01L3/00 , F04B43/04 , F04B53/16 , F16L19/06 , F16L49/06 , G01N30/36 , G01N30/60 , F16L17/02
CPC分类号： B01J19/0093 , B01J2219/0081 , B01L3/502715 , B01L3/563 , B01L2200/027 , B01L2200/0689 , B01L2300/14 , F04B43/043 , F04B53/16 , G01N30/36 , G01N30/6026 , G01N30/6078 , G01N30/6095
摘要： A high pressure connector capable of operating at pressures of 40,000 psi or higher is provided. This connector can be employed to position a first fluid-bearing conduit that has a proximal end and a distal end to a second fluid-bearing conduit thereby providing fluid communication between the first and second fluid-bearing conduits. The connector includes (a) an internal fitting assembly having a body cavity with (i) a lower segment that defines a lower segment aperture and (ii) an interiorly threaded upper segment, (b) a first member having a first member aperture that traverses its length wherein the first member aperture is configured to accommodate the first fluid-bearing conduit and wherein the first member is positioned in the lower segment of the internal fitting assembly, and (c) a second member having a second member aperture that traverses its length wherein the second member is positioned in the upper segment of the fitting assembly and wherein a lower surface of the second member is in contact with an upper surface of the first member to assert a compressive force onto the first member and wherein the first member aperture and the second member aperture are coaxial.
摘要翻译： 提供能够在40,000psi或更高压力下操作的高压连接器。 该连接器可用于将具有近端和远端的第一流体承载管道定位到第二流体承载导管，从而提供第一和第二流体承载导管之间的流体连通。 连接器包括（a）具有体腔的内部配件组件，其具有限定下部分段孔的下部段和（ii）内部带螺纹的上部段，（b）第一构件，其具有穿过第一构件孔 其长度，其中所述第一构件孔被构造成容纳所述第一流体承载导管，并且其中所述第一构件定位在所述内部装配组件的下部段中，以及（c）第二构件，其具有穿过其长度的第二构件孔 其中所述第二构件定位在所述装配组件的上部段中，并且其中所述第二构件的下表面与所述第一构件的上表面接触以将压缩力施加到所述第一构件上，并且其中所述第一构件孔和 第二构件孔是同轴的。
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公开(公告)号：US08585986B1
公开(公告)日：2013-11-19
申请号：US11983440
申请日：2007-11-09
申请人： Ronald F. Renzi
发明人： Ronald F. Renzi
IPC分类号： B01L99/00
CPC分类号： F16L39/00 , B01L3/565 , B01L2200/027 , B01L2300/0838 , G01N2030/185
摘要： An interconnecting device for connecting a plurality of first fluid-bearing conduits to a corresponding plurality of second fluid-bearing conduits thereby providing fluid communication between the first fluid-bearing conduits and the second fluid-bearing conduits. The device includes a manifold and one or two ferrule plates that are held by compressive axial forces.
摘要翻译： 一种互连装置，用于将多个第一流体承载管道连接到相应的多个第二流体承载导管，从而在第一流体承载管道和第二流体承载导管之间提供流体连通。 该装置包括通过压缩轴向力保持的歧管和一个或两个套圈板。
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8.发明授权Particle sorter comprising a fluid displacer in a closed-loop fluid circuit 有权颗粒分选机包括在闭环流体回路中的流体置换器公开(公告)号：US08162149B1
公开(公告)日：2012-04-24
申请号：US12424758
申请日：2009-04-16
IPC分类号： B07C5/38
CPC分类号： B01L3/502761 , B01L3/502784 , B01L2200/0636 , B01L2200/0652 , B01L2300/0816 , B01L2300/0864 , B01L2300/088 , B01L2400/0439 , B01L2400/0487 , G01N15/1484 , G01N2015/1006 , G01N2015/149
摘要： Disclosed herein are methods and devices utilizing a fluid displacer in a closed-loop fluid circuit.
摘要翻译： 本文公开了在闭环流体回路中利用流体置换器的方法和装置。
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公开(公告)号：US20120004144A1
公开(公告)日：2012-01-05
申请号：US13173180
申请日：2011-06-30
IPC分类号： C40B60/12
CPC分类号： B01L3/502761 , B01L2200/0668 , B01L2300/0645 , B01L2300/0864 , C12M35/02 , Y10T436/2575
摘要： We have developed an microelectroporation device that combines microarrays of oligonucleotides, microfluidic channels, and electroporation for cell transfection and high-throughput screening applications (e.g. RNA interference screens). Microarrays allow the deposition of thousands of different oligonucleotides in microscopic spots. Microfluidic channels and microwells enable efficient loading of cells into the device and prevent cross-contamination between different oligonucleotides spots. Electroporation allows optimal transfection of nucleic acids into cells (especially hard-to-transfect cells such as primary cells) by minimizing cell death while maximizing transfection efficiency. This invention has the advantage of a higher throughput and lower cost, while preventing cross-contamination compared to conventional screening technologies. Moreover, this device does not require bulky robotic liquid handling equipment and is inherently safer given that it is a closed system.
摘要翻译： 我们开发了一种微电极装置，其结合寡核苷酸微阵列，微流体通道和电穿孔用于细胞转染和高通量筛选应用（例如RNA干扰筛选）。 微阵列允许在微观斑点中沉积数千种不同的寡核苷酸。 微流体通道和微孔能够有效地将细胞装载到装置中并防止不同寡核苷酸斑点之间的交叉污染。 电穿孔允许通过最大限度地减少细胞死亡同时使转染效率最大化将核酸最佳地转染到细胞（特别是难转染细胞，如原代细胞）中。 本发明具有较高的生产能力和较低的成本，同时防止与常规筛选技术相比的交叉污染。 此外，该装置不需要庞大的机器人液体处理设备，并且由于它是封闭的系统，所以固有地更安全。
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公开(公告)号：US07311882B1
公开(公告)日：2007-12-25
申请号：US10350541
申请日：2003-01-24
申请人： Ronald F. Renzi
发明人： Ronald F. Renzi
IPC分类号： B01L11/00
CPC分类号： F16L39/00 , B01L3/565 , B01L2200/027 , B01L2300/0838 , G01N2030/185
摘要： A manifold for connecting external capillaries to the inlet and/or outlet ports of a microfluidic device for high pressure applications is provided. The fluid connector for coupling at least one fluid conduit to a corresponding port of a substrate that includes: (i) a manifold comprising one or more channels extending therethrough wherein each channel is at least partially threaded, (ii) one or more threaded ferrules each defining a bore extending therethrough with each ferrule supporting a fluid conduit wherein each ferrule is threaded into a channel of the manifold, (iii) a substrate having one or more ports on its upper surface wherein the substrate is positioned below the manifold so that the one or more ports is aligned with the one or more channels of the manifold, and (iv) means for applying an axial compressive force to the substrate to couple the one or more ports of the substrate to a corresponding proximal end of a fluid conduit.
摘要翻译： 提供了用于将外部毛细管连接到用于高压应用的微流体装置的入口和/或出口端口的歧管。 用于将至少一个流体导管联接到基板的对应端口的流体连接器，其包括：（i）包括一个或多个延伸穿过其中的通道的歧管，其中每个通道至少部分地具有螺纹，（ii）一个或多个螺纹套圈 限定一个延伸穿过其中的孔，其中每个套圈支撑流体导管，其中每个套圈拧入歧管的通道中，（iii）在其上表面上具有一个或多个端口的衬底，其中衬底位于歧管下方， 或多个端口与歧管的一个或多个通道对准，以及（iv）用于向衬底施加轴向压缩力以将衬底的一个或多个端口耦合到流体导管的相应近端的装置。
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