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    Materials and methods for detection of nucleic acids
    2.
    发明授权
    Materials and methods for detection of nucleic acids 有权
    用于检测核酸的材料和方法

    公开(公告)号：US07422850B2

    公开(公告)日：2008-09-09

    申请号：US09861292

    申请日：2001-05-18

    申请人： David J. Marshall ,  James R. Prudent ,  Christopher B. Sherrill ,  Gideon Shapiro ,  Jennifer K. Grenier ,  Craig S. Richmond ,  Simona Jurczyk ,  Jerod L. Ptacin

    发明人： David J. Marshall ,  James R. Prudent ,  Christopher B. Sherrill ,  Gideon Shapiro ,  Jennifer K. Grenier ,  Craig S. Richmond ,  Simona Jurczyk ,  Jerod L. Ptacin

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6853 ,  C12Q1/6823 ,  C12Q1/6827 ,  C12Q1/6832 ,  C12Q1/6834 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2565/1015 ,  C12Q2525/101 ,  C12Q2525/161

    摘要： Assays using non-natural bases are described. In one embodiment, the method involves contacting a sample suspected of containing the target nucleic acid with a polymerase and first and second primers; amplifying the target nucleic acid, if present in the sample, by PCR using the first and second primers to generate an amplification product having a double-stranded region and a single-stranded region that comprises the non-natural base; contacting the sample with a reporter comprising a label and a non-natural base that is complementary to the non-natural base of the single-stranded region; annealing at least a portion of the reporter to the single-stranded region of the amplification product; cleaving, after annealing, at least a portion of the reporter to release at least one reporter fragment; and correlating the release of the at least one reporter fragment with the presence of the target nucleic acid in the sample. The invention also provides corresponding kits for use in detecting target nucleic acids in a sample. Alternatively, the reporter can be incorporated into the amplification product rather than annealing and then cleaving.

    摘要翻译： 描述了使用非天然碱的测定法。 在一个实施方案中，所述方法包括使怀疑含有靶核酸的样品与聚合酶和第一和第二引物接触; 如果存在于样品中，则使用第一和第二引物通过PCR扩增靶核酸以产生具有双链区域和包含非天然碱基的单链区域的扩增产物; 使样品与包含与单链区域的非天然碱基互补的标记物和非天然碱基的报告子接触; 将至少一部分报告物退火至扩增产物的单链区域; 在退火后切割至少一部分报告物以释放至少一个报道片段; 并将至少一个报道片段的释放与样品中靶核酸的存在相关联。 本发明还提供用于检测样品中靶核酸的相应试剂盒。 或者，可以将报道子并入扩增产物，而不是退火然后裂解。

    Non-peptide somatostatin receptor ligands
    5.
    发明授权
    Non-peptide somatostatin receptor ligands 失效
    非肽生长抑素受体配体

    公开(公告)号：US07189856B2

    公开(公告)日：2007-03-13

    申请号：US10289924

    申请日：2002-11-07

    申请人： Gideon Shapiro ,  Michael G. Natchus ,  Mark A. Lockwood ,  Simona Jurczyk

    发明人： Gideon Shapiro ,  Michael G. Natchus ,  Mark A. Lockwood ,  Simona Jurczyk

    IPC分类号： C07D27/104 ,  A61K31/41

    CPC分类号： C07D403/06

    摘要： Non-peptide somatostatin receptor ligands with conformationally restricted side chains exhibiting high binding affinity toward somatostatin receptors are provided. The compounds exhibit a high selectivity and act as agonists at human subtype 2 somatostatin receptors. The compounds are long acting for advantageous use as medicaments in peripheral diseases where somatostatinergic therapy is indicated. Furthermore, many of the compounds are lipophilic and are particularly useful for treating central nervous system and ophthalmic diseases where penetration of the blood brain and blood retinal barriers is required. It is a further object to describe the preferred stereoisomers of these somatostatin agonists and processes for their preparation. Further objects will become apparent from reading the following description.

    摘要翻译： 提供了具有对生长抑素受体具有高结合亲和力的构象限制性侧链的非肽生长抑素受体配体。 化合物表现出高选择性，并且作为人类2型生长抑素受体的激动剂。 化合物长效作为有益用途，作为指示生长抑素治疗的外周疾病中的药物。 此外，许多化合物是亲脂性的，并且特别可用于治疗中枢神经系统和需要渗透血脑屏障和血液视网膜屏障的眼科疾病。 另一个目的是描述这些生长抑素激动剂的优选立体异构体及其制备方法。 通过阅读以下描述，其他目的将变得显而易见。