公开(公告)号：US20110203013A1

公开(公告)日：2011-08-18

申请号：US13012095

申请日：2011-01-24

申请人： David J. Peterson ,  William James Gordon-Kamm

发明人： David J. Peterson ,  William James Gordon-Kamm

IPC分类号： A01H1/06 ,  C12N15/82 ,  C12N5/04 ,  C12N5/10 ,  B82Y5/00

CPC分类号： C12N15/895 ,  B82Y5/00 ,  C12N15/8207

摘要： Methods and mixtures for providing a composition of interest to a plant cell are provided. Methods using a mixture of particles are provided, wherein the mixture includes at least one nanoparticle associated with a microparticle via a lipid compound. Methods include methods for plant cell transformation, methods to increase transformation frequency, methods to increase gene targeting, and methods for plastid transformation. The nanoparticle comprises at least one composition of interest. Also provided are transformed plant cells, plants, and seeds produced using the methods and mixtures described herein. Further provided are the methods of making and using the mixtures of particles.

摘要翻译： 提供了用于提供植物细胞感兴趣的组合物的方法和混合物。 提供了使用颗粒混合物的方法，其中所述混合物包括通过脂质化合物与微粒缔合的至少一种纳米颗粒。 方法包括植物细胞转化的方法，增加转化频率的方法，增加基因靶向的方法以及质体转化方法。 纳米颗粒包含至少一种目标组合物。 还提供了使用本文所述的方法和混合物转化的转化的植物细胞，植物和种子。 进一步提供制造和使用颗粒混合物的方法。

公开(公告)号：US08383891B2

公开(公告)日：2013-02-26

申请号：US13168133

申请日：2011-06-24

申请人： Rebecca E. Cahoon ,  William James Gordon-Kamm ,  Keith S. Lowe ,  Christopher Jay Scelonge ,  Yumin Tao

发明人： Rebecca E. Cahoon ,  William James Gordon-Kamm ,  Keith S. Lowe ,  Christopher Jay Scelonge ,  Yumin Tao

IPC分类号： C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8241 ,  C07K14/415 ,  C12N15/62 ,  C12N15/8201 ,  C12N15/8207 ,  C12N15/8261 ,  Y02A40/146

摘要： This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.

公开(公告)号：US07994391B2

公开(公告)日：2011-08-09

申请号：US12018292

申请日：2008-01-23

申请人： Rebecca E. Cahoon ,  William James Gordon-Kamm ,  Yumin Tao ,  Keith S. Lowe ,  Christopher Jay Scelonge

发明人： Rebecca E. Cahoon ,  William James Gordon-Kamm ,  Yumin Tao ,  Keith S. Lowe ,  Christopher Jay Scelonge

IPC分类号： C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8241 ,  C07K14/415 ,  C12N15/62 ,  C12N15/8201 ,  C12N15/8207 ,  C12N15/8261 ,  Y02A40/146

摘要： This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.

摘要翻译： 本发明涉及编码WUS多肽的分离的多核苷酸。 本发明还提供了分离的WUS多肽。 本发明还提供了使用多核苷酸来调节WUS水平，提高转化效率，刺激植物细胞生长（包括干细胞）刺激器官发生，刺激体细胞胚发生，诱导无融合生殖以及提供阳性选择的方法 包含多核苷酸的细胞。 本发明还涉及包含本发明的多核苷酸或通过本发明的方法产生的细胞，植物和种子。

公开(公告)号：US07897383B2

公开(公告)日：2011-03-01

申请号：US12256721

申请日：2008-10-23

申请人： Anthony J. Kinney ,  Kimberly F. Glassman ,  William James Gordon-Kamm ,  Keith S. Lowe ,  Scott E. Nichols ,  Kevin L. Stecca

发明人： Anthony J. Kinney ,  Kimberly F. Glassman ,  William James Gordon-Kamm ,  Keith S. Lowe ,  Scott E. Nichols ,  Kevin L. Stecca

IPC分类号： C07H21/02 ,  C07H21/04 ,  C12Q1/68

CPC分类号： C12N15/8216 ,  C12N15/8218 ,  C12N15/8245 ,  C12N15/8246 ,  C12N15/8247 ,  C12N15/825 ,  C12N15/8251

摘要： Recombinant constructs useful for reducing the expression of endogenous mRNA and any substantially similar endogenous mRNA are disclosed. In particular, a recombinant construct comprising, inter alia, a suitable nucleic acid sequence and its reverse complement can be used to alter the expression of any homologous, endogenous RNA (i.e., the target RNA) which is in proximity to this suitable nucleic acid sequence.

摘要翻译： 公开了可用于减少内源性mRNA的表达和任何基本上相似的内源mRNA的重组构建体。 特别地，包含尤其是合适的核酸序列及其反向互补序列的重组构建体可用于改变任何同源的内源RNA（即靶RNA）的表达，其接近该合适的核酸序列 。

公开(公告)号：US07348468B1

公开(公告)日：2008-03-25

申请号：US09807946

申请日：2000-09-28

申请人： Rebecca E. Cahoon ,  Keith Lowe ,  William James Gordon-Kamm ,  Yumin Tao ,  Christopher Jay Scelonge

发明人： Rebecca E. Cahoon ,  Keith Lowe ,  William James Gordon-Kamm ,  Yumin Tao ,  Christopher Jay Scelonge

IPC分类号： C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8241 ,  C07K14/415 ,  C12N15/62 ,  C12N15/8201 ,  C12N15/8207 ,  C12N15/8261 ,  Y02A40/146

摘要： This invention relates to an isolated nucleic acid fragment encoding a WUS protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the WUS protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the WUS protein in a transformed host cell.

摘要翻译： 本发明涉及编码WUS蛋白的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码WUS蛋白的全部或一部分的嵌合基因，其中嵌合基因的表达导致转化宿主细胞中WUS蛋白水平的改变。