CELL PROCESSING METHOD AND CELL PROCESSING APPARATUS

    公开(公告)号:US20240309403A1

    公开(公告)日:2024-09-19

    申请号:US18676661

    申请日:2024-05-29

    IPC分类号: C12N15/89

    CPC分类号: C12N15/895

    摘要: The cell processing method includes a step of passing a liquid containing a cell through an orifice from a flow path by a pressure applying unit, and is characterized in that the orifice is connected to the flow path so as to narrow a flow from the flow path and in that the orifice includes at least any one of a portion in which an area of a cross-section perpendicular to a flow direction of the liquid passing through a center of the orifice is prevented from being changed from an inlet toward an outlet of the orifice or a portion in which the area is increased from the inlet toward the outlet of the orifice. The cell processing apparatus is characterized by including an orifice forming member having the orifice, the flow path, and the pressure applying unit.

    Inkjet gene printing
    3.
    发明授权

    公开(公告)号:US09598704B2

    公开(公告)日:2017-03-21

    申请号:US14697704

    申请日:2015-04-28

    摘要: Provided herein are methods and apparatuses for transfecting a cell with a compound of interest by stressing the cell, e.g. with shear stress. The compound of interest may be nucleic acids, proteins, molecules, nanoparticles, drugs, etc., or any combination thereof. Methods of printing cells with an inkjet printing device are also provided, wherein at least a portion of viable cells (preferably at least 1%) are transfected with a compound of interest. Preferably, at least 25% of the cells are viable after printing. In addition, methods of forming an array of viable cells are provided wherein at least a portion of the viable printed cells (preferably at least 1%) are transfected with at least one compound of interest.

    Transportation device
    4.
    发明授权
    Transportation device 有权
    运输装置

    公开(公告)号:US09453243B2

    公开(公告)日:2016-09-27

    申请号:US14190479

    申请日:2014-02-26

    申请人: Chien-Lung Chen

    摘要: A transportation device, transporting a material to a target, includes an input module, a transmission module and an output module. The input module has a containing unit and at least one filter. The at least one filter is connected to the containing unit, and a carrier fluid and the material are stored in the containing unit. The transmission module is coupled to the at least one filter of the input module. The output module has a guiding unit, a throat portion, a first opening and a second opening. The throat portion is positioned between the first opening and the second opening. One end of the guiding unit is connected to the transmission module. The other end of the guiding unit has a guiding corner for connecting to the first opening. The material will enter the output module through the first opening and reaches the target through the second opening.

    摘要翻译: 将材料输送到目标的运输装置包括输入模块,传输模块和输出模块。 输入模块具有容纳单元和至少一个滤波器。 至少一个过滤器连接到容纳单元,并且载体流体和材料存储在容纳单元中。 传输模块耦合到输入模块的至少一个滤波器。 输出模块具有引导单元,喉部,第一开口和第二开口。 喉部位于第一开口和第二开口之间。 引导单元的一端连接到传输模块。 引导单元的另一端具有用于连接到第一开口的引导拐角。 材料将通过第一个开口进入输出模块,并通过第二个开口到达目标。

    Inkjet gene printing
    5.
    发明授权
    Inkjet gene printing 有权
    喷墨基因印刷

    公开(公告)号:US09045757B2

    公开(公告)日:2015-06-02

    申请号:US12663020

    申请日:2008-06-06

    摘要: Provided herein are methods and apparatuses for transfecting a cell with a compound of interest by stressing the cell, e.g. with shear stress. The compound of interest may be nucleic acids, proteins, molecules, nanoparticles, drugs, etc., or any combination thereof. Methods of printing cells with an inkjet printing device are also provided, wherein at least a portion of viable cells (preferably at least 1%) are transfected with a compound of interest. Preferably, at least 25% of the cells are viable after printing. In addition, methods of forming an array of viable cells are provided wherein at least a portion of the viable printed cells (preferably at least 1%) are transfected with at least one compound of interest.

    摘要翻译: 本文提供了用于通过施加细胞来转染具有感兴趣的化合物的细胞的方法和装置,例如, 具有剪切应力。 感兴趣的化合物可以是核酸,蛋白质,分子,纳米颗粒,药物等,或其任何组合。 还提供了用喷墨印刷装置印刷细胞的方法,其中至少一部分活细胞(优选至少1%)用感兴趣的化合物转染。 优选地,至少25%的细胞在印刷后是活的。 此外,提供形成活细胞阵列的方法,其中至少一部分活的印刷细胞(优选至少1%)用至少一种目标化合物转染。

    LOW-PRESSURE BIOLISTIC BARRELS
    7.
    发明申请
    LOW-PRESSURE BIOLISTIC BARRELS 审中-公开
    低压生物线条

    公开(公告)号:US20120135526A1

    公开(公告)日:2012-05-31

    申请号:US13300288

    申请日:2011-11-18

    申请人: Kenneth Greenberg

    发明人: Kenneth Greenberg

    IPC分类号: A61M37/00 C12N15/87 C12M1/00

    摘要: Low pressure biolistic barrels and biolistic devices including the same are provided. Aspects of the biolistic barrels include the presence of one or more pressure-reducing elements. Also provided are kits which include the biolistic barrels, as well as methods of delivering a molecule to a target site with the biolistic barrels and devices that include the same. The devices and methods described herein find use in a variety of applications, including in vivo and in vitro high-precision delivery applications.

    摘要翻译: 提供了低压生物弹药桶和包括其的生物弹药装置。 生物弹药桶的方面包括存在一个或多个减压元件。 还提供了包括生物弹药桶的试剂盒,以及使用生物弹药筒和包括其的装置将分子递送到靶位点的方法。 本文描述的装置和方法可用于各种应用,包括体内和体外高精度递送应用。

    DNA LOADED SUPPORTED GOLD NANOPARTICLES, PROCESS FOR THE PREPARATION AND USE THEREOF
    8.
    发明申请
    DNA LOADED SUPPORTED GOLD NANOPARTICLES, PROCESS FOR THE PREPARATION AND USE THEREOF 有权
    DNA负载金黄色纳米颗粒,其制备和使用过程

    公开(公告)号:US20120129223A1

    公开(公告)日:2012-05-24

    申请号:US13388616

    申请日:2010-08-03

    IPC分类号: C12N15/63 C12N15/64 B82Y5/00

    CPC分类号: C12N15/895 B82Y5/00

    摘要: The present invention relates to DNA loaded gold nanoparticles embedded in sharp carbonaceous carriers useful for higher DNA delivery efficiently into plants. These nanogold embedded carbon matrices are prepared by heat treatment of biogenic intracellular gold nanoparticles. The DNA delivery efficiency is tested on model plants. These materials reveal good dispersion of the transport material, producing a greater number of GUS foci per unit area. The added advantages of the composite carrier are the lower plasmid and gold requirements. Plant cell damage with the prepared carbon supported particles is very minimal and can be gauged from the increased plant regeneration and transformation efficiency compared to that of the commercial micrometer sized gold particles. This can be attributed to the sharp edges that the carbon supports possess, which lead to better piercing capabilities with minimum damage.

    摘要翻译: 本发明涉及用于有效进入植物的较高DNA递送的锋利碳质载体中的负载DNA的金纳米颗粒。 通过热处理生物源细胞内金纳米颗粒制备这些纳米金嵌入碳基质。 在模型植物上测试DNA递送效率。 这些材料表现出良好的运输材料分散性,每单位面积产生更多的GUS焦点。 复合载体的附加优点是较低的质粒和金要求。 用制备的碳负载颗粒的植物细胞损伤非常小,可以从商业微米尺寸金颗粒的植物再生和转化效率的增加中测量。 这可归因于碳支撑具有的锋利边缘,这导致更好的穿孔能力并具有最小的损伤。

    VACCINE
    10.
    发明申请
    VACCINE 审中-公开
    疫苗

    公开(公告)号:US20090104231A1

    公开(公告)日:2009-04-23

    申请号:US12174715

    申请日:2008-07-17

    IPC分类号: A61K39/00

    摘要: The present invention relates to methods and compositions useful in the treatment and prevention of Hepatitis C virus (HCV) infections and the symptoms and diseases associated therewith. In particular the present invention relates to DNA vaccines comprising polynucleotide sequences encoding HCV proteins, and methods of treatment of individuals infected with HCV comprising administration of the vaccines of the present invention.

    摘要翻译: 本发明涉及可用于治疗和预防丙型肝炎病毒(HCV)感染的方法和组合物以及与之相关的症状和疾病。 特别地,本发明涉及包含编码HCV蛋白质的多核苷酸序列的DNA疫苗,以及治疗感染HCV的个体的方法,包括施用本发明的疫苗。