摘要:
The cell processing method includes a step of passing a liquid containing a cell through an orifice from a flow path by a pressure applying unit, and is characterized in that the orifice is connected to the flow path so as to narrow a flow from the flow path and in that the orifice includes at least any one of a portion in which an area of a cross-section perpendicular to a flow direction of the liquid passing through a center of the orifice is prevented from being changed from an inlet toward an outlet of the orifice or a portion in which the area is increased from the inlet toward the outlet of the orifice. The cell processing apparatus is characterized by including an orifice forming member having the orifice, the flow path, and the pressure applying unit.
摘要:
Improved biolistic bombardment is observable when using a modification to a double-barrel device. One such modification is a custom attachment that can be retrofit to a standard helium-based gene gun. The custom attachment facilitates DNA and protein delivery. The attachment confines the flow of particles in a barrel similar to the choke on a shotgun barrel.
摘要:
Provided herein are methods and apparatuses for transfecting a cell with a compound of interest by stressing the cell, e.g. with shear stress. The compound of interest may be nucleic acids, proteins, molecules, nanoparticles, drugs, etc., or any combination thereof. Methods of printing cells with an inkjet printing device are also provided, wherein at least a portion of viable cells (preferably at least 1%) are transfected with a compound of interest. Preferably, at least 25% of the cells are viable after printing. In addition, methods of forming an array of viable cells are provided wherein at least a portion of the viable printed cells (preferably at least 1%) are transfected with at least one compound of interest.
摘要:
A transportation device, transporting a material to a target, includes an input module, a transmission module and an output module. The input module has a containing unit and at least one filter. The at least one filter is connected to the containing unit, and a carrier fluid and the material are stored in the containing unit. The transmission module is coupled to the at least one filter of the input module. The output module has a guiding unit, a throat portion, a first opening and a second opening. The throat portion is positioned between the first opening and the second opening. One end of the guiding unit is connected to the transmission module. The other end of the guiding unit has a guiding corner for connecting to the first opening. The material will enter the output module through the first opening and reaches the target through the second opening.
摘要:
Provided herein are methods and apparatuses for transfecting a cell with a compound of interest by stressing the cell, e.g. with shear stress. The compound of interest may be nucleic acids, proteins, molecules, nanoparticles, drugs, etc., or any combination thereof. Methods of printing cells with an inkjet printing device are also provided, wherein at least a portion of viable cells (preferably at least 1%) are transfected with a compound of interest. Preferably, at least 25% of the cells are viable after printing. In addition, methods of forming an array of viable cells are provided wherein at least a portion of the viable printed cells (preferably at least 1%) are transfected with at least one compound of interest.
摘要:
To provide a transformation method for producing a stramenopile organism having an improved unsaturated fatty acid production capability by disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner. [Solution] A method for transforming a stramenopile organism, which comprises disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner, and which is characterized in that the stramenopile organism is selected from Thraustochytrium aureum, Parietichytrium sarkarianum, Thraustochytrium roseum and Parietichytrium sp. and the gene to be disrupted or of which the expression is to be inhibited is a gene associated with the biosynthesis of a fatty acid.
摘要:
Low pressure biolistic barrels and biolistic devices including the same are provided. Aspects of the biolistic barrels include the presence of one or more pressure-reducing elements. Also provided are kits which include the biolistic barrels, as well as methods of delivering a molecule to a target site with the biolistic barrels and devices that include the same. The devices and methods described herein find use in a variety of applications, including in vivo and in vitro high-precision delivery applications.
摘要:
The present invention relates to DNA loaded gold nanoparticles embedded in sharp carbonaceous carriers useful for higher DNA delivery efficiently into plants. These nanogold embedded carbon matrices are prepared by heat treatment of biogenic intracellular gold nanoparticles. The DNA delivery efficiency is tested on model plants. These materials reveal good dispersion of the transport material, producing a greater number of GUS foci per unit area. The added advantages of the composite carrier are the lower plasmid and gold requirements. Plant cell damage with the prepared carbon supported particles is very minimal and can be gauged from the increased plant regeneration and transformation efficiency compared to that of the commercial micrometer sized gold particles. This can be attributed to the sharp edges that the carbon supports possess, which lead to better piercing capabilities with minimum damage.
摘要:
A needleless syringe having a membrane (28) which is ruptured by gas pressure to generate a supersonic gas flow in which particles containing a therapeutic agent are injected.
摘要:
The present invention relates to methods and compositions useful in the treatment and prevention of Hepatitis C virus (HCV) infections and the symptoms and diseases associated therewith. In particular the present invention relates to DNA vaccines comprising polynucleotide sequences encoding HCV proteins, and methods of treatment of individuals infected with HCV comprising administration of the vaccines of the present invention.