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    Seed-preferred regulatory elements and uses thereof
    6.
    发明授权
    Seed-preferred regulatory elements and uses thereof 失效
    种子优选的调节元件及其用途

    公开(公告)号：US07432418B2

    公开(公告)日：2008-10-07

    申请号：US11519184

    申请日：2006-09-11

    申请人： Shane E. Abbitt ,  William J. Gordon-Kamm ,  Keith S. Lowe

    发明人： Shane E. Abbitt ,  William J. Gordon-Kamm ,  Keith S. Lowe

    IPC分类号： C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12Y505/01004 ,  C07K14/415 ,  C12N9/90 ,  C12N15/8234

    摘要： The present invention provides compositions and methods for regulating expression of isolated nucleotide sequences in a plant. The compositions are novel nucleic acid sequences for callus-tissue and seed embryo-preferred regulatory sequences. Methods for expressing an isolated nucleotide sequence in a plant or callus tissue using the regulatory sequences are also provided. The methods comprise transforming a plant cell to contain an isolated nucleotide sequence operably linked to the regulatory sequences of the present invention and regenerating a stably transformed plant or callus tissue from the transformed plant cell.

    摘要翻译： 本发明提供用于调节植物中分离的核苷酸序列表达的组合物和方法。 组合物是用于愈伤组织和种子胚优选调节序列的新型核酸序列。 还提供了使用调节序列在植物或愈伤组织中表达分离的核苷酸序列的方法。 所述方法包括将植物细胞转化成含有与本发明的调控序列可操作地连接的分离的核苷酸序列，并从转化的植物细胞再生稳定转化的植物或愈伤组织。

    Methods and compositions for targeted polynucleotide modification
    10.
    发明授权
    Methods and compositions for targeted polynucleotide modification 有权
    用于靶向多核苷酸修饰的方法和组合物

    公开(公告)号：US08704041B2

    公开(公告)日：2014-04-22

    申请号：US12982013

    申请日：2010-12-30

    申请人： William J. Gordon-Kamm ,  Keith S. Lowe ,  David J. Peterson ,  Christopher J. Scelonge ,  Grace M. St. Clair ,  Bing-Bing Wang

    发明人： William J. Gordon-Kamm ,  Keith S. Lowe ,  David J. Peterson ,  Christopher J. Scelonge ,  Grace M. St. Clair ,  Bing-Bing Wang

    IPC分类号： C12N15/82 ,  C12N15/87 ,  C12N5/04 ,  C12N5/14 ,  C07H21/02

    CPC分类号： C12N15/8216 ,  C07K14/415 ,  C12N15/8213

    摘要： A variety of methods and compostions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism.

    摘要翻译： 提供了各种方法和组合物，包括用于靶向修饰细胞或生物体中特定靶位点的方法和组合物，用于整合感兴趣的多核苷酸的方法，评估启动子活性的方法，直接选择转化的生物体，最小化或消除产生的表达 从随机整合到生物体如植物的基因组中，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，使基因沉默，并鉴定和/或表征转录调节区。 该方法涉及将细胞增殖因子和双链断裂诱导酶引入生物体中。