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    Zea mays (L.) with capability of long term, highly efficient plant regeneration including fertile transgenic maize plants having a heterologous gene, and their preparation
    1.
    发明授权
    Zea mays (L.) with capability of long term, highly efficient plant regeneration including fertile transgenic maize plants having a heterologous gene, and their preparation 失效
    具有长期，高效植物再生能力的玉米（L.），包括具有异源基因的可育转基因玉米植物及其制备

    公开(公告)号：US06284945B1

    公开(公告)日：2001-09-04

    申请号：US08897736

    申请日：1997-07-21

    申请人： Denes Dudits ,  Sandor Morocz ,  Janos Nemeth ,  Günter Donn

    发明人： Denes Dudits ,  Sandor Morocz ,  Janos Nemeth ,  Günter Donn

    IPC分类号： A01H500

    CPC分类号： A01H5/10 ,  A01H4/00 ,  A01H4/005 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8206 ,  C12N15/8277

    摘要： Protoplasts which regenerate reproducibly in a short time to normal, fertile plants can be regenerated from an auxin-autotrophic genotype of Zea mays (L.). Starting from immature embryos on hormone-free media, an auxin-autotrophic, embryogenic callus is formed on the shoot basis of the seedlings, which callus retains its embryogenic potential over a substantial period of time when subcultured on hormone-free medium. In addition to fully-developed embryos, adventitious embryos are also formed under suitable culture conditions (6-9% of sucrose in the medium). When the sucrose content is reduced to 2-3% and 2,4-dichlorophenoxyacetic acid is added, soft, granular calli are formed which consist of embryogenic cell aggregates (type II callus). After subculturing the type II callus in the form of a cell suspension culture, totipotent protoplasts can be isolated. From these protoplasts, the maize plants according to the invention are regenerated.

    摘要翻译： 在短时间内可再生再生的正常肥沃植物的原生质体可以从玉米的生长素 - 自养基因型（L.）中再生。 从无激素培养基上的未成熟胚开始，在幼苗的基础上形成生长素 - 自养的胚性愈伤组织，所述愈伤组织在无激素培养基上传代培养的相当长的时间内保留其胚胎发生潜力。 除了完全发育的胚胎之外，在合适的培养条件（培养基中为6-9％的蔗糖）中也形成不定胚。 当蔗糖含量降低至2-3％，加入2,4-二氯苯氧乙酸时，形成由胚胎细胞聚集体（II型愈伤组织）组成的软质颗粒愈伤组织。 以细胞悬浮培养物形式传代培养II型愈伤组织后，可以分离全能的原生质体。 从这些原生质体中，再生根据本发明的玉米植物。

    Zea mays (L.) with capability of long term, highly efficient plant regeneration including fertile transgenic maize plants having a heterologous gene, and their preparation
    3.
    发明授权
    Zea mays (L.) with capability of long term, highly efficient plant regeneration including fertile transgenic maize plants having a heterologous gene, and their preparation 失效
    具有长期，高效植物再生能力的玉米（L.），包括具有异源基因的可育转基因玉米植物及其制备

    公开(公告)号：US5792936A

    公开(公告)日：1998-08-11

    申请号：US462840

    申请日：1995-06-05

    申请人： Denes Dudits ,  Sandor Morocz ,  Janos Nemeth ,  Gunter Donn

    发明人： Denes Dudits ,  Sandor Morocz ,  Janos Nemeth ,  Gunter Donn

    IPC分类号： A01H4/00 ,  A01H5/10 ,  C12N5/04 ,  C12N9/10 ,  C12N15/82 ,  A01H5/00

    CPC分类号： A01H4/00 ,  A01H4/005 ,  A01H5/10 ,  C12N15/8206 ,  C12N15/8277 ,  C12N5/04 ,  C12N9/1029

    摘要： Protoplasts which regenerate reproducibly in a short time to normal, fertile plants can be regenerated from an auxin-autotrophic genotype of Zea mays (L.). Starting from immature embryos on hormone-free media, an auxin-autotrophic, embryogenic callus is formed on the shoot basis of the seedlings, which callus retains its embryogenic potential over a substantial period of time when subcultured on hormone-free medium. In addition to fully-developed embryos, adventitious embryos are also formed under suitable culture conditions (6-9% of sucrose in the medium). When the sucrose content is reduced to 2-3% and 2,4-dichlorophenoxyacetic acid is added, soft, granular calli are formed which consist of embryogenic cell aggregates (type II callus). After subculturing the type II callus in the form of a cell suspension culture, totipotent protoplasts can be isolated. From these protoplasts, the maize plants according to the invention are regenerated.

    摘要翻译： 可以在短时间内可再生再生的正常肥沃植物的原生质体可以从玉米的生长素 - 自养基因型（L.）中再生。 从无激素培养基上的未成熟胚胎开始，在幼苗的基础上形成生长素 - 自养的胚性愈伤组织，所述愈伤组织在无激素培养基上传代培养的相当长的时间内保持其胚胎发生潜力。 除了完全发育的胚胎之外，在合适的培养条件（培养基中为6-9％的蔗糖）中也形成不定胚。 当蔗糖含量降低至2-3％，加入2,4-二氯苯氧乙酸时，形成由胚胎细胞聚集体（II型愈伤组织）组成的软质颗粒愈伤组织。 以细胞悬浮培养物形式传代培养II型愈伤组织后，可以分离全能的原生质体。 从这些原生质体中，再生根据本发明的玉米植物。

    Plants having improved growth characteristics and method for making the same
    5.
    发明申请
    Plants having improved growth characteristics and method for making the same 失效
    具有改善生长特性的植物及其制备方法

    公开(公告)号：US20050210544A1

    公开(公告)日：2005-09-22

    申请号：US11084250

    申请日：2005-03-21

    申请人： Attila Feher ,  Denes Dudits ,  Valerie Frankard

    发明人： Attila Feher ,  Denes Dudits ,  Valerie Frankard

    IPC分类号： A01H1/00 ,  C07K14/415 ,  C12N15/82

    CPC分类号： C12N15/8261 ,  C07K14/415 ,  Y02A40/146

    摘要： The present invention concerns a method for improving the growth characteristics of plants by introducing and expressing in a plant a nucleic acid encoding an S3A protein. The invention also relates to transgenic plants having introduced therein a nucleic acid encoding an S3A protein, which plants have improved growth characteristics relative to corresponding wild type plants. The present invention also concerns constructs useful in the methods of the invention.

    摘要翻译： 本发明涉及通过在植物中引入和表达编码S3A蛋白的核酸来改善植物生长特性的方法。 本发明还涉及其中引入了编码S3A蛋白的核酸的转基因植物，该植物相对于相应的野生型植物具有改善的生长特性。 本发明还涉及可用于本发明方法的构建体。

    Plants having improved seed yield and expressing a nucleic acid encoding a small subunit ribosomal (S3A) protein and method for making the same
    10.
    发明授权
    Plants having improved seed yield and expressing a nucleic acid encoding a small subunit ribosomal (S3A) protein and method for making the same 失效
    具有提高种子产量并表达编码小亚基核糖体（S3A）蛋白质的核酸的植物及其制备方法

    公开(公告)号：US07579518B2

    公开(公告)日：2009-08-25

    申请号：US11084250

    申请日：2005-03-21

    申请人： Attila Feher ,  Denes Dudits ,  Valerie Frankard

    发明人： Attila Feher ,  Denes Dudits ,  Valerie Frankard

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8261 ,  C07K14/415 ,  Y02A40/146

    摘要： The present invention concerns a method for improving the growth characteristics of plants by introducing and expressing in a plant a nucleic acid encoding a small subunit ribosomal (S3a) protein. The invention also relates to transgenic plants having introduced therein a nucleic acid encoding an S3a protein, which plants have improved growth characteristics relative to corresponding wild type plants. The improved growth characteristics are particularly increased seed yield compared to corresponding wild type plants, in particularly increased number of filled seeds, increased total seed weight, increased harvest index and increased thousand kernel weight. The present invention also concerns constructs useful in the methods of the invention.

    摘要翻译： 本发明涉及通过在植物中引入和表达编码小亚基核糖体（S3a）蛋白的核酸来改善植物生长特性的方法。 本发明还涉及其中引入了编码S3a蛋白的核酸的转基因植物，该植物相对于相应的野生型植物具有改善的生长特性。 与相应的野生型植物相比，改良的生长特性特别增加，种子数量特别增加，种子总重量增加，收获指数增加，千粒重增加。 本发明还涉及可用于本发明方法的构建体。