公开(公告)号：US08734832B2

公开(公告)日：2014-05-27

申请号：US13621174

申请日：2012-09-15

申请人： Derek O'Hagan ,  Gary S. Ott ,  John Donnelly ,  Jina Kazzaz ,  Mildred Ugozzoli ,  Manmohan Singh ,  John Barackman

发明人： Derek O'Hagan ,  Gary S. Ott ,  John Donnelly ,  Jina Kazzaz ,  Mildred Ugozzoli ,  Manmohan Singh ,  John Barackman

IPC分类号： A61F2/02 ,  A61K9/14 ,  A61K9/50

CPC分类号： A61K39/39 ,  A61K9/1075 ,  A61K9/167 ,  A61K39/0208 ,  A61K39/21 ,  A61K39/245 ,  A61K39/29 ,  Y02A50/411 ,  Y02A50/471

摘要： Microparticles with adsorbent surfaces, methods of making such microparticles, and uses thereof, are disclosed. The microparticles comprise a polymer, such as a poly(α-hydroxy acid), a polyhydroxy butyric acid, a polycaprolactone, a polyorthoester, a polyanhydride, and the like, and are formed using cationic, anionic, or nonionic detergents. The surface of the microparticles efficiently adsorb biologically active macromolecules, such as DNA, polypeptides, antigens, and adjuvants. Also provided are compositions of an oil droplet emulsion having a metabolizable oil and an emulsifying agent. Immunogenic compositions having an immunostimulating amount of an antigenic substance, and an immunostimulating amount of an adjuvant composition are also provided. Methods of stimulating an immune response, methods of immunizing a host animal against a viral, bacterial, or parasitic infection, and methods of increasing a Th1 immune response in a host animal by administering to the animal an immunogenic composition of the microparticles, and/or microemulsions of the invention, are also provided.

公开(公告)号：US20130195898A1

公开(公告)日：2013-08-01

申请号：US13621174

申请日：2012-09-15

申请人： Derek O'Hagan ,  Gary S. Ott ,  John Donnelly ,  Jina Kazzaz ,  Mildred Ugozzoli ,  Manmohan Singh ,  John Barackman

发明人： Derek O'Hagan ,  Gary S. Ott ,  John Donnelly ,  Jina Kazzaz ,  Mildred Ugozzoli ,  Manmohan Singh ,  John Barackman

IPC分类号： A61K39/39

CPC分类号： A61K39/39 ,  A61K9/1075 ,  A61K9/167 ,  A61K39/0208 ,  A61K39/21 ,  A61K39/245 ,  A61K39/29 ,  Y02A50/411 ,  Y02A50/471

摘要： Microparticles with adsorbent surfaces, methods of making such microparticles, and uses thereof, are disclosed. The microparticles comprise a polymer, such as a poly(α-hydroxy acid), a polyhydroxy butyric acid, a polycaprolactone, a polyorthoester, a polyanhydride, and the like, and are formed using cationic, anionic, or nonionic detergents. The surface of the microparticles efficiently adsorb biologically active macromolecules, such as DNA, polypeptides, antigens, and adjuvants. Also provided are compositions of an oil droplet emulsion having a metabolizable oil and an emulsifying agent. Immunogenic compositions having an immunostimulating amount of an antigenic substance, and an immunostimulating amount of an adjuvant composition are also provided. Methods of stimulating an immune response, methods of immunizing a host animal against a viral, bacterial, or parasitic infection, and methods of increasing a Th1 immune response in a host animal by administering to the animal an immunogenic composition of the microparticles, and/or microemulsions of the invention, are also provided.

公开(公告)号：US08206749B1

公开(公告)日：2012-06-26

申请号：US09914279

申请日：2000-02-09

申请人： Derek O'Hagan ,  Gary S. Ott ,  John Donnelly ,  Jina Kazzaz ,  Mildred Ugozzoli ,  Manmohan Singh ,  John Barackman

发明人： Derek O'Hagan ,  Gary S. Ott ,  John Donnelly ,  Jina Kazzaz ,  Mildred Ugozzoli ,  Manmohan Singh ,  John Barackman

IPC分类号： A61K9/50

CPC分类号： A61K39/39 ,  A61K9/1075 ,  A61K9/167 ,  A61K39/0208 ,  A61K39/21 ,  A61K39/245 ,  A61K39/29 ,  Y02A50/411 ,  Y02A50/471

摘要： Microparticles with adsorbent surfaces, methods of making such microparticles, and uses thereof, are disclosed. The microparticles comprise a polymer, such as a poly(α-hydroxy acid), a polyhydroxy butyric acid, a polycaprolactone, a polyorthoester, a polyanhydride, and the like, and are formed using cationic, anionic, or nonionic detergents. The surface of the microparticles efficiently adsorb biologically active macromolecules, such as DNA, polypeptides, antigens, and adjuvants. Also provided are compositions of an oil droplet emulsion having a metabolizable oil and an emulsifying agent. Immunogenic compositions having an immunostimulating amount of an antigenic substance, and an immunostimulating amount of an adjuvant composition are also provided. Methods of stimulating an immune response, methods of immunizing a host animal against a viral, bacterial, or parasitic infection, and methods of increasing a Th1 immune response in a host animal by administering to the animal an immunogenic composition of the microparticles, and/or microemulsions of the invention, are also provided.

公开(公告)号：US08771747B2

公开(公告)日：2014-07-08

申请号：US13618020

申请日：2012-09-14

申请人： Derek O'Hagan ,  Gary S. Ott ,  John Donnelly ,  Jina Kazzaz ,  Mildred Ugozzoli ,  Manmohan Singh ,  John Barackman

发明人： Derek O'Hagan ,  Gary S. Ott ,  John Donnelly ,  Jina Kazzaz ,  Mildred Ugozzoli ,  Manmohan Singh ,  John Barackman

IPC分类号： A61K9/50 ,  A61K39/39 ,  A61K39/21 ,  A61K9/107 ,  A61K39/29 ,  A61K39/02 ,  A61K39/245 ,  A61K9/16

CPC分类号： A61K39/39 ,  A61K9/1075 ,  A61K9/167 ,  A61K39/0208 ,  A61K39/21 ,  A61K39/245 ,  A61K39/29 ,  Y02A50/411 ,  Y02A50/471

摘要： Microparticles with adsorbent surfaces, methods of making such microparticles, and uses thereof, are disclosed. The microparticles comprise a polymer, such as a poly(α-hydroxy acid), a polyhydroxy butyric acid, a polycaprolactone, a polyorthoester, a polyanhydride, and the like, and are formed using cationic, anionic, or nonionic detergents. The surface of the microparticles efficiently adsorb biologically active macromolecules, such as DNA, polypeptides, antigens, and adjuvants. Also provided are compositions of an oil droplet emulsion having a metabolizable oil and an emulsifying agent. Immunogenic compositions having an immunostimulating amount of an antigenic substance, and an immunostimulating amount of an adjuvant composition are also provided. Methods of stimulating an immune response, methods of immunizing a host animal against a viral, bacterial, or parasitic infection, and methods of increasing a Th1 immune response in a host animal by administering to the animal an immunogenic composition of the microparticles, and/or microemulsions of the invention, are also provided.

摘要翻译： 公开了具有吸附剂表面的微粒，制备这种微粒的方法及其用途。 微粒包含聚（α-羟基酸），多羟基丁酸，聚己内酯，聚原酸酯，聚酐等聚合物，并使用阳离子，阴离子或非离子型洗涤剂形成。 微粒的表面有效吸附生物活性大分子，如DNA，多肽，抗原和佐剂。 还提供了具有可代谢油和乳化剂的油滴乳液的组合物。 还提供了具有免疫刺激量的抗原物质的免疫原性组合物和免疫刺激量的佐剂组合物。 刺激免疫应答的方法，免疫宿主动物免受病毒，细菌或寄生虫感染的方法，以及通过向动物施用微粒的免疫原性组合物和/或增加宿主动物的Th1免疫应答的方法 还提供了本发明的微乳液。

公开(公告)号：US20130195923A1

公开(公告)日：2013-08-01

申请号：US13618020

申请日：2012-09-14

申请人： Derek O'Hagan ,  Gary S. Ott ,  John Donnelly ,  Jina Kazzaz ,  Mildred Ugozzoli ,  Manmohan Singh ,  John Barackman

发明人： Derek O'Hagan ,  Gary S. Ott ,  John Donnelly ,  Jina Kazzaz ,  Mildred Ugozzoli ,  Manmohan Singh ,  John Barackman

IPC分类号： A61K9/107 ,  A61K39/39 ,  A61K39/245 ,  A61K39/02 ,  A61K39/21 ,  A61K39/29

CPC分类号： A61K39/39 ,  A61K9/1075 ,  A61K9/167 ,  A61K39/0208 ,  A61K39/21 ,  A61K39/245 ,  A61K39/29 ,  Y02A50/411 ,  Y02A50/471

摘要： Microparticles with adsorbent surfaces, methods of making such microparticles, and uses thereof, are disclosed. The microparticles comprise a polymer, such as a poly(α-hydroxy acid), a polyhydroxy butyric acid, a polycaprolactone, a polyorthoester, a polyanhydride, and the like, and are formed using cationic, anionic, or nonionic detergents. The surface of the microparticles efficiently adsorb biologically active macromolecules, such as DNA, polypeptides, antigens, and adjuvants. Also provided are compositions of an oil droplet emulsion having a metabolizable oil and an emulsifying agent. Immunogenic compositions having an immunostimulating amount of an antigenic substance, and an immunostimulating amount of an adjuvant composition are also provided. Methods of stimulating an immune response, methods of immunizing a host animal against a viral, bacterial, or parasitic infection, and methods of increasing a Th1 immune response in a host animal by administering to the animal an immunogenic composition of the microparticles, and/or microemulsions of the invention, are also provided.

摘要翻译： 公开了具有吸附剂表面的微粒，制备这种微粒的方法及其用途。 微粒包含聚（α-羟基酸），多羟基丁酸，聚己内酯，聚原酸酯，聚酐等聚合物，并使用阳离子，阴离子或非离子型洗涤剂形成。 微粒的表面有效吸附生物活性大分子，如DNA，多肽，抗原和佐剂。 还提供了具有可代谢油和乳化剂的油滴乳液的组合物。 还提供了具有免疫刺激量的抗原物质的免疫原性组合物和免疫刺激量的佐剂组合物。 刺激免疫应答的方法，免疫宿主动物免受病毒，细菌或寄生虫感染的方法，以及通过向动物施用微粒的免疫原性组合物和/或增加宿主动物的Th1免疫应答的方法 还提供了本发明的微乳液。

公开(公告)号：US08309139B2

公开(公告)日：2012-11-13

申请号：US11406144

申请日：2006-04-18

申请人： Derek O'Hagan ,  Gary S. Ott ,  John Donnelly ,  Jina Kazzaz ,  Mildred Ugozzoli ,  Manmohan Singh ,  John Barackman

发明人： Derek O'Hagan ,  Gary S. Ott ,  John Donnelly ,  Jina Kazzaz ,  Mildred Ugozzoli ,  Manmohan Singh ,  John Barackman

IPC分类号： A61K9/50 ,  A61K9/14

CPC分类号： A61K39/39 ,  A61K9/1075 ,  A61K9/167 ,  A61K39/0208 ,  A61K39/21 ,  A61K39/245 ,  A61K39/29 ,  Y02A50/411 ,  Y02A50/471

摘要： Microparticles with adsorbent surfaces, methods of making such microparticles, and uses thereof, are disclosed. The microparticles comprise a polymer, such as a poly(α-hydroxy acid), a polyhydroxy butyric acid, a polycaprolactone, a polyorthoester, a polyanhydride, and the like, and are formed using cationic, anionic, or nonionic detergents. The surface of the microparticles efficiently adsorb biologically active macromolecules, such as DNA, polypeptides, antigens, and adjuvants. Also provided are compositions of an oil droplet emulsion having a metabolizable oil and an emulsifying agent. Immunogenic compositions having an immunostimulating amount of an antigenic substance, and an immunostimulating amount of an adjuvant composition are also provided. Methods of stimulating an immune response, methods of immunizing a host animal against a viral, bacterial, or parasitic infection, and methods of increasing a Th1 immune response in a host animal by administering to the animal an immunogenic composition of the microparticles, and/or microemulsions of the invention, are also provided.

摘要翻译： 公开了具有吸附剂表面的微粒，制备这种微粒的方法及其用途。 微粒包含聚（α-羟基酸），多羟基丁酸，聚己内酯，聚原酸酯，聚酐等聚合物，并使用阳离子，阴离子或非离子型洗涤剂形成。 微粒的表面有效吸附生物活性大分子，如DNA，多肽，抗原和佐剂。 还提供了具有可代谢油和乳化剂的油滴乳液的组合物。 还提供了具有免疫刺激量的抗原物质的免疫原性组合物和免疫刺激量的佐剂组合物。 刺激免疫应答的方法，免疫宿主动物免受病毒，细菌或寄生虫感染的方法，以及通过向动物施用微粒的免疫原性组合物和/或增加宿主动物的Th1免疫应答的方法 还提供了本发明的微乳液。

公开(公告)号：US20070116709A1

公开(公告)日：2007-05-24

申请号：US11406144

申请日：2006-04-18

申请人： Derek O'Hagan ,  Gary Ott ,  John Donnelly ,  Jina Kazzaz ,  Mildred Ugozzoli ,  Manmohan Singh ,  John Barackman

发明人： Derek O'Hagan ,  Gary Ott ,  John Donnelly ,  Jina Kazzaz ,  Mildred Ugozzoli ,  Manmohan Singh ,  John Barackman

IPC分类号： A61K39/00 ,  A61K39/12 ,  A61K39/21 ,  A61K39/145

CPC分类号： A61K39/39 ,  A61K9/1075 ,  A61K9/167 ,  A61K39/0208 ,  A61K39/21 ,  A61K39/245 ,  A61K39/29 ,  Y02A50/411 ,  Y02A50/471

摘要： Microparticles with adsorbent surfaces, methods of making such microparticles, and uses thereof, are disclosed. The microparticles comprise a polymer, such as a poly(α-hydroxy acid), a polyhydroxy butyric acid, a polycaprolactone, a polyorthoester, a polyanhydride, and the like, and are formed using cationic, anionic, or nonionic detergents. The surface of the microparticles efficiently adsorb biologically active macromolecules, such as DNA, polypeptides, antigens, and adjuvants. Also provided are compositions of an oil droplet emulsion having a metabolizable oil and an emulsifying agent. Immunogenic compositions having an immunostimulating amount of an antigenic substance, and an immunostimulating amount of an adjuvant composition are also provided. Methods of stimulating an immune response, methods of immunizing a host animal against a viral, bacterial, or parasitic infection, and methods of increasing a Th1 immune response in a host animal by administering to the animal an immunogenic composition of the microparticles, and/or microemulsions of the invention, are also provided.

摘要翻译： 公开了具有吸附剂表面的微粒，制备这种微粒的方法及其用途。 微粒包含聚（α-羟基酸），多羟基丁酸，聚己内酯，聚原酸酯，聚酐等聚合物，并使用阳离子，阴离子或非离子型洗涤剂形成。 微粒的表面有效吸附生物活性大分子，如DNA，多肽，抗原和佐剂。 还提供了具有可代谢油和乳化剂的油滴乳液的组合物。 还提供了具有免疫刺激量的抗原物质的免疫原性组合物和免疫刺激量的佐剂组合物。 刺激免疫应答的方法，免疫宿主动物免受病毒，细菌或寄生虫感染的方法，以及通过向动物施用微粒的免疫原性组合物和/或增加宿主动物的Th1免疫应答的方法 还提供了本发明的微乳液。

公开(公告)号：US20080026071A1

公开(公告)日：2008-01-31

申请号：US11653792

申请日：2007-01-16

申请人： Derek O'Hagan ,  Gillis Otten ,  John Donnelly ,  John Polo ,  Susan Barnett ,  Manmohan Singh ,  Jeffrey Ulmer ,  Thomas Dubensky

发明人： Derek O'Hagan ,  Gillis Otten ,  John Donnelly ,  John Polo ,  Susan Barnett ,  Manmohan Singh ,  Jeffrey Ulmer ,  Thomas Dubensky

IPC分类号： A61K39/21 ,  A61K39/00 ,  A61K39/12 ,  A61K39/125 ,  A61K39/29 ,  A61P31/04 ,  A61P33/00 ,  A61P31/12 ,  A61K9/50 ,  A61K39/215 ,  A61K39/145 ,  A61K39/155

CPC分类号： C12N15/86 ,  A61K9/1647 ,  A61K9/167 ,  A61K39/00 ,  A61K39/12 ,  A61K39/21 ,  A61K47/6927 ,  A61K2039/53 ,  A61K2039/545 ,  A61K2039/55505 ,  A61K2039/55555 ,  A61K2039/55566 ,  A61K2039/57 ,  A61K2039/6093 ,  C12N2740/16134 ,  C12N2740/16234 ,  C12N2770/36143 ,  Y02A50/388

摘要： Microparticles with adsorbent surfaces, methods of making such microparticles, and uses thereof, are disclosed. The microparticles comprise a polymer, such as a poly(α-hydroxy acid), a polyhydroxy butyric acid, a polycaprolactone, a polyorthoester, a polyanhydride, and the like, and are formed using cationic, anionic, or nonionic detergents. Also provided are microparticles in the form of submicron emulsions of an oil droplet emulsion having a metabolizable oil and an emulsifying agent. The surface of the microparticles efficiently adsorb polypeptides, such as antigens, and nucleic acids, such as ELVIS vectors and other vector constructs, containing heterologous nucleotide sequences encoding biologically active macromolecules, such as polypeptides, antigens, and adjuvants. Methods of stimulating an immune response, methods of immunizing a host animal against a viral, bacterial, or parasitic infection, and uses of the microparticle compositions for vaccines are also provided.

摘要翻译： 公开了具有吸附剂表面的微粒，制备这种微粒的方法及其用途。 微粒包含聚（α-羟基酸），多羟基丁酸，聚己内酯，聚原酸酯，聚酐等聚合物，并使用阳离子，阴离子或非离子型洗涤剂形成。 还提供了具有可代谢油和乳化剂的油滴乳液的亚微米乳液形式的微粒。 微粒的表面有效地吸收多肽，如抗原和核酸，如ELVIS载体和其他载体构建体，其含有编码生物活性大分子如多肽，抗原和佐剂的异源核苷酸序列。 还提供了刺激免疫应答的方法，免疫宿主动物免受病毒，细菌或寄生虫感染的方法，以及微粒组合物用于疫苗的用途。

公开(公告)号：US20090238841A1

公开(公告)日：2009-09-24

申请号：US12364294

申请日：2009-02-02

申请人： John Donnelly ,  Susan W. Barnett ,  Derek O'Hagan

发明人： John Donnelly ,  Susan W. Barnett ,  Derek O'Hagan

IPC分类号： A61K39/21 ,  C12P21/02 ,  A61K31/713 ,  A61P31/18 ,  A61K31/711

CPC分类号： A61K39/21 ,  A61K39/12 ,  A61K48/0041 ,  A61K48/005 ,  A61K48/0083 ,  A61K2039/53 ,  A61K2039/545 ,  A61K2039/55566 ,  A61K2039/57 ,  A61K2039/6093 ,  C07K14/005 ,  C12N15/87 ,  C12N2740/16034 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16222 ,  C12N2740/16234

摘要： Provided herein are HIV vaccines comprising HIV polypeptide-encoding DNA adsorbed to PLG and/or HIV proteins. Also provided are methods of using these vaccines to generate immune responses in a subject.

摘要翻译： 本文提供包含吸附于PLG和/或HIV蛋白质的编码HIV多肽的DNA的HIV疫苗。 还提供了使用这些疫苗在受试者中产生免疫应答的方法。

公开(公告)号：US08609109B2

公开(公告)日：2013-12-17

申请号：US13334739

申请日：2011-12-22

申请人： John Donnelly ,  Susan W. Barnett ,  Derek O'Hagan

发明人： John Donnelly ,  Susan W. Barnett ,  Derek O'Hagan

IPC分类号： A61K39/21 ,  A61P37/04

CPC分类号： A61K39/21 ,  A61K39/12 ,  A61K48/0041 ,  A61K48/005 ,  A61K48/0083 ,  A61K2039/53 ,  A61K2039/545 ,  A61K2039/55566 ,  A61K2039/57 ,  A61K2039/6093 ,  C07K14/005 ,  C12N15/87 ,  C12N2740/16034 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2740/16222 ,  C12N2740/16234

摘要： Provided herein are HIV vaccines comprising HIV polypeptide-encoding DNA adsorbed to PLG and/or HIV proteins. Also provided are methods of using these vaccines to generate immune responses in a subject.

摘要翻译： 本文提供包含吸附于PLG和/或HIV蛋白质的编码HIV多肽的DNA的HIV疫苗。 还提供了使用这些疫苗在受试者中产生免疫应答的方法。