-
公开(公告)号：US06806058B2
公开(公告)日：2004-10-19
申请号：US10157351
申请日：2002-05-28
IPC分类号： G01N3353
CPC分类号： G01N33/5432 , C07K16/00 , C07K2317/52 , G01N33/5436 , G01N33/56966 , G01N33/56972 , Y10S436/823
摘要： The invention provides methods of analzying a secreted protein from a cell encapsulated in a microdrop. The microdrop is formulated with biotinylated matrix molecules at a reduced ratio of biotin to matrix molecules compared with previous formulations. The reduced ratio is advantageous for improving the resolution of detection and allows simultaneous detection of multiple secreted proteins and/or multiple cell surface markers. The invention further provides inter alia methods of isolating IgG isotype antibodies that have switched from IgM isotype.
摘要翻译： 本发明提供了从包封在微滴中的细胞中分泌分泌蛋白质的方法。 与以前的制剂相比，微滴与生物素基质分子配制，生物素与基质分子的比例降低。 减少的比例对于改善检测的分辨率是有利的，并且允许同时检测多种分泌蛋白质和/或多种细胞表面标志物。 本发明进一步提供了分离从IgM同种型转换的IgG同种型抗体的方法。
-
公开(公告)号：US07556928B2
公开(公告)日：2009-07-07
申请号：US10917673
申请日：2004-08-13
IPC分类号： G01N33/53
CPC分类号： G01N33/5432 , C07K16/00 , C07K2317/52 , G01N33/5436 , G01N33/56966 , G01N33/56972 , Y10S436/823
摘要： The invention provides methods of analyzing a secreted protein from a cell encapsulated in a microdrop. The microdrop is formulated with biotinylated matrix molecules at a reduced ratio of biotin to matrix molecules compared with previous formulations. The reduced ratio is advantageous for improving the resolution of detection and allows simultaneous detection of multiple secreted proteins and/or multiple cell surface markers. The invention further provides inter alia methods of isolating IgG isotype antibodies that have switched from IgM isotype.
摘要翻译： 本发明提供了从包封在微滴中的细胞分析分泌蛋白质的方法。 与以前的制剂相比，微滴与生物素基质分子配制，生物素与基质分子的比例降低。 减少的比例对于改善检测的分辨率是有利的，并且允许同时检测多种分泌蛋白质和/或多种细胞表面标志物。 本发明进一步提供了分离从IgM同种型转换的IgG同种型抗体的方法。
-
公开(公告)号：US20050019839A1
公开(公告)日：2005-01-27
申请号：US10917673
申请日：2004-08-13
IPC分类号： G01N33/53 , C07K16/00 , C12P21/08 , C12Q1/02 , G01N33/543 , G01N33/566 , G01N33/567 , G01N33/569
CPC分类号： G01N33/5432 , C07K16/00 , C07K2317/52 , G01N33/5436 , G01N33/56966 , G01N33/56972 , Y10S436/823
摘要： The invention provides methods of analzying a secreted protein from a cell encapsulated in a microdrop. The microdrop is formulated with biotinylated matrix molecules at a reduced ratio of biotin to matrix molecules compared with previous formulations. The reduced ratio is advantageous for improving the resolution of detection and allows simultaneous detection of multiple secreted proteins and/or multiple cell surface markers. The invention further provides inter alia methods of isolating IgG isotype antibodies that have switched from IgM isotype.
摘要翻译： 本发明提供了从包封在微滴中的细胞中分泌分泌蛋白质的方法。 与以前的制剂相比，微滴与生物素基质分子配制，生物素与基质分子的比例降低。 减少的比例对于改善检测的分辨率是有利的，并且允许同时检测多种分泌蛋白质和/或多种细胞表面标志物。 本发明进一步提供了分离从IgM同种型转换的IgG同种型抗体的方法。
-
公开(公告)号：US20080171329A1
公开(公告)日：2008-07-17
申请号：US11925705
申请日：2007-10-26
申请人： Jan Trnovsky , Patricia McGrath
发明人： Jan Trnovsky , Patricia McGrath
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6834 , C12Q1/68 , C12Q1/6813 , C12Q1/6816 , C12Q1/6827 , C12Q1/6841 , C12Q1/689 , C12Q1/70 , C12Q2600/156 , C12Q2600/158 , C12Q2523/101 , C12Q2565/501 , C12Q2563/161
摘要： The invention provides methods of nucleic acid analysis. Such methods entail forming a population of gel microdrops encapsulating a population of biological entities, each entity comprising a nucleic acid, whereby at least some microdrops in the population each encapsulate a single entity. The population of gel microdrops is then contacted with a probe under conditions whereby the probe specifically hybridizes to at least one complementary sequence in the nucleic acid in at least one gel microdrop. At least one gel microdrop is then analyzed or detected. The biological entities can be cells, viruses, nuclei and chromosomes.
摘要翻译： 本发明提供核酸分析方法。 这样的方法需要形成包封生物实体群体的凝胶微滴群，每个实体包含核酸，其中群体中的至少一些微滴包封单个实体。 然后将凝胶微滴的群体与探针接触，其中探针与至少一种凝胶微滴中核酸中的至少一个互补序列特异性杂交。 然后分析或检测至少一个凝胶微滴。 生物实体可以是细胞，病毒，细胞核和染色体。
-
公开(公告)号：US20080160498A1
公开(公告)日：2008-07-03
申请号：US11868139
申请日：2007-10-05
申请人： Jan Trnovsky , Patricia McGrath
发明人： Jan Trnovsky , Patricia McGrath
CPC分类号： C12Q1/6834 , C12Q1/68 , C12Q1/6813 , C12Q1/6816 , C12Q1/6827 , C12Q1/6841 , C12Q1/689 , C12Q1/70 , C12Q2600/156 , C12Q2600/158 , C12Q2523/101 , C12Q2565/501 , C12Q2563/161
摘要： The invention provides methods of nucleic acid analysis. Such methods entail forming a population of gel microdrops encapsulating a population of biological entities, each entity comprising a nucleic acid, whereby at least some microdrops in the population each encapsulate a single entity. The population of gel microdrops is then contacted with a probe under conditions whereby the probe specifically hybridizes to at least one complementary sequence in the nucleic acid in at least one gel microdrop. At least one gel microdrop is then analyzed or detected. The biological entities can be cells, viruses, nuclei and chromosomes.
摘要翻译： 本发明提供核酸分析方法。 这样的方法需要形成包封生物实体群体的凝胶微滴群，每个实体包含核酸，其中群体中的至少一些微滴包封单个实体。 然后将凝胶微滴的群体与探针接触，其中探针与至少一种凝胶微滴中核酸中的至少一个互补序列特异性杂交。 然后分析或检测至少一个凝胶微滴。 生物实体可以是细胞，病毒，细胞核和染色体。
-
公开(公告)号：US20070020617A1
公开(公告)日：2007-01-25
申请号：US11278659
申请日：2006-04-04
申请人： Jan Trnovsky , Patricia McGrath
发明人： Jan Trnovsky , Patricia McGrath
CPC分类号： C12Q1/6834 , C12Q1/68 , C12Q1/6813 , C12Q1/6816 , C12Q1/6827 , C12Q1/6841 , C12Q1/689 , C12Q1/70 , C12Q2600/156 , C12Q2600/158 , C12Q2523/101 , C12Q2565/501 , C12Q2563/161
摘要： The invention provides methods of nucleic acid analysis. Such methods entail forming a population of gel microdrops encapsulating a population of biological entities, each entity comprising a nucleic acid, whereby at least some microdrops in the population each encapsulate a single entity. The population of gel microdrops is then contacted with a probe under conditions whereby the probe specifically hybridizes to at least one complementary sequence in the nucleic acid in at least one gel microdrop. At least one gel microdrop is then analyzed or detected. The biological entities can be cells, viruses, nuclei and chromosomes.
摘要翻译： 本发明提供核酸分析方法。 这样的方法需要形成包封生物实体群体的凝胶微滴群，每个实体包含核酸，其中群体中的至少一些微滴包封单个实体。 然后将凝胶微滴的群体与探针接触，其中探针与至少一种凝胶微滴中核酸中的至少一个互补序列特异性杂交。 然后分析或检测至少一个凝胶微滴。 生物实体可以是细胞，病毒，细胞核和染色体。
-
公开(公告)号：US06586176B1
公开(公告)日：2003-07-01
申请号：US09369640
申请日：1999-08-06
申请人： Jan Trnovsky , Patricia McGrath
发明人： Jan Trnovsky , Patricia McGrath
IPC分类号： C12Q168
CPC分类号： C12Q1/6834 , C12Q1/68 , C12Q1/6813 , C12Q1/6816 , C12Q1/6827 , C12Q1/6841 , C12Q1/689 , C12Q1/70 , C12Q2600/156 , C12Q2600/158 , C12Q2523/101 , C12Q2565/501 , C12Q2563/161
摘要： The invention provides methods of nucleic acid analysis. Such methods entail forming a population of gel microdrops encapsulating a population of biological entities, each entity comprising a nucleic acid, whereby at least some microdrops in the population each encapsulate a single entity. The population of gel microdrops is then contacted with a probe under conditions whereby the probe specifically hybridizes to at least one complementary sequence in the nucleic acid in at least one gel microdrop. At least one gel microdrop is then analyzed or detected. The biological entities can be cells, viruses, nuclei and chromosomes.
摘要翻译： 本发明提供核酸分析方法。 这样的方法需要形成包封生物实体群体的凝胶微滴群，每个实体包含核酸，其中群体中的至少一些微滴包封单个实体。 然后将凝胶微滴的群体与探针接触，其中探针与至少一种凝胶微滴中核酸中的至少一个互补序列特异性杂交。 然后分析或检测至少一个凝胶微滴。 生物实体可以是细胞，病毒，细胞核和染色体。
-
公开(公告)号：US20120052499A1
公开(公告)日：2012-03-01
申请号：US13319685
申请日：2010-05-20
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/6841 , G01N33/56911 , G01N33/56938 , C12Q2525/107 , C12Q2537/143 , C12Q2565/102
摘要： This application pertains to methods for the whole-cell analysis of gram-positive bacteria. The methods are capable of making a determination of whether or not a sample (e.g. a clinical sample) comprises one or more select gram-positive bacteria as well as, for example, whether or not none, some or all of said select gram-positive bacteria in said sample, or possibly other bacteria in said sample, possess a select trait or traits of interest. In some embodiments, the methods can be used to determine methicillin-resistant (the select trait) staphylococcus aureus (the select gram-positive bacteria), coagulase-negative staphylococci (another select gram-positive bacteria) and/or methicillin-sensitive staphylococcus aureus (MSSA) in said sample. The whole-cell analysis can be performed, for example, by in-situ hybridization (ISH), fluorescence in-situ hybridization (FISH), immunocytochemistry (ICC), or any combination of two or more of the foregoing.
摘要翻译： 本申请涉及革兰氏阳性细菌的全细胞分析方法。 所述方法能够确定样品（例如临床样品）是否包含一种或多种选择性革兰氏阳性细菌，以及例如是否一种或全部所述选择性革兰氏阳性细菌 所述样品中的细菌或所述样品中可能的其它细菌具有选择性状或感兴趣的性状。 在一些实施方案中，所述方法可用于测定耐甲氧西林（选择性状）金黄色葡萄球菌（选择性革兰氏阳性菌），凝固酶阴性葡萄球菌（另一种选择革兰氏阳性菌）和/或甲氧西林敏感性金黄色葡萄球菌 （MSSA）。 全细胞分析可以例如通过原位杂交（ISH），荧光原位杂交（FISH），免疫细胞化学（ICC）或两种或更多种前述的任何组合进行。
-
公开(公告)号：US20050059049A1
公开(公告)日：2005-03-17
申请号：US10886315
申请日：2004-07-07
申请人： Phillip Moen , Jan Trnovsky
发明人： Phillip Moen , Jan Trnovsky
IPC分类号： C07H21/04 , C12N20060101 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6816 , C12Q2525/301 , C12Q2525/313
摘要： The present invention provides nucleic acid hybridization probes having a target-binding region and a labeled hairpin structure at at least one end of the probe. The hairpin-labeled probes include oligonucleotides, dendrimers, and primer-extended nucleic acids. The probes can be used in disclosed methods for detection of target nucleic acids. In addition, the oligonucleotide probes can be used in disclosed methods for primer-extension, including, e.g., random priming and PCR amplification, to produce the primer-extended hairpin-labeled probes. Also disclosed are kits comprising the hairpin-labeled oligonucleotide and dendrimer probes. Further, the present invention provides biomolecules (e.g., peptides, polypeptides, carbohydrates, lipids, and the like) that are labeled via linkage to labeled hairpin structures.
摘要翻译： 本发明提供在探针的至少一端具有靶结合区和标记的发夹结构的核酸杂交探针。 发夹标记的探针包括寡核苷酸，树枝状大分子和引物延伸的核酸。 探针可用于公开的靶核酸检测方法中。 此外，寡核苷酸探针可用于公开的引物延伸方法，包括例如随机引发和PCR扩增，以产生引物延伸的发夹标记的探针。 还公开了包含发夹标记的寡核苷酸和树状聚合物探针的试剂盒。 此外，本发明提供通过与标记的发夹结构连接而标记的生物分子（例如肽，多肽，碳水化合物，脂质等）。
-
-
-
-
-
-
-
-
搜索结果
9 条记录 (耗时 0.031 秒)
