公开(公告)号：US06573081B2

公开(公告)日：2003-06-03

申请号：US09947551

申请日：2001-09-07

申请人： Dieter Bernhardt ,  Thomas Weimer ,  Albrecht Groener

发明人： Dieter Bernhardt ,  Thomas Weimer ,  Albrecht Groener

IPC分类号： C12N700

CPC分类号： G01N33/56983 ,  A61K39/12 ,  A61K2039/525 ,  A61K2039/5254 ,  C12N7/00 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2750/10064

摘要： Methods for growing circoviruses, in particular porcine circoviruses, which are obtained from an infected cell culture after one or more passages in cultures of porcine, bovine or human cells are described. When the porcine circoviruses grow, a cytopathogenic effect occurs in the cell culture.

摘要翻译： 描述了在猪，牛或人细胞培养物中一次或多次传代之后从受感染细胞培养物获得的生长圆环病毒，特别是猪圆环病毒的方法。 当猪圆环病毒生长时，在细胞培养物中发生致细胞致病作用。

公开(公告)号：US06183999B2

公开(公告)日：2001-02-06

申请号：US09198243

申请日：1998-11-24

申请人： Thomas Weimer ,  Albrecht Groener

发明人： Thomas Weimer ,  Albrecht Groener

IPC分类号： C12P1934

CPC分类号： C12Q1/701

摘要： A procedure for the detection of high virus concentrations in blood plasma and/or blood serum by means of the polymerase chain reaction (PCR) is described, in which the sensitivity of the PCR is restricted by the use of suboptimal nucleic acid extraction, amplification or detection conditions. This procedure is suitable, for example, for the detection of parvovirus DNA in plasma or serum, it being possible to adjust the sensitivity of the PCR such that the parvovirus DNA is only detected in samples whose DNA content is greater than 106 to 107 genome equivalents/ml. The detection of the parvovirus DNA amplification carried out is carried out in the sample by measurements of the fluorescence.

摘要翻译： 描述了通过聚合酶链式反应（PCR）检测血浆和/或血清中高病毒浓度的方法，其中PCR的灵敏度通过使用次最佳核酸提取，扩增或 检测条件。 该方法例如用于检测血浆或血清中的细小病毒DNA，可以调节PCR的灵敏度，使得仅在DNA含量大于106至107个基因组当量的样品中检测到细小病毒DNA / ml。 通过荧光测量在样品中进行细小病毒DNA扩增的检测。

公开(公告)号：US20070231843A1

公开(公告)日：2007-10-04

申请号：US11711020

申请日：2007-02-27

申请人： Martin Vey ,  Wiegand Lang ,  Albrecht Groener ,  Anne Bellon

发明人： Martin Vey ,  Wiegand Lang ,  Albrecht Groener ,  Anne Bellon

IPC分类号： C07K16/18 ,  C07K14/00 ,  G01N33/53

CPC分类号： G01N33/6896 ,  C07K16/18 ,  G01N2800/2828

摘要： This application includes description of antibodies for specifically detecting prions of human origin and methods for detecting pathogenic prions. In some embodiments, the antibodies bind to an epitope characteristic of a human prion protein which is not found in the prion proteins of other species. In some embodiments, the antibodies are not cross-reactive with cow, Syrian Gold hamster, mouse, or rat prions. The application also includes a conformation-dependent immunoassay method for detecting pathogenic prions in a sample containing a prion (PrP) protein. The PrP protein may be present in a first conformation and a second conformation, such as PrPc and PrPSc in which the two conformations have different binding affinity for the antibody used to detect them.

摘要翻译： 本申请包括用于特异性检测人源性朊病毒的抗体的描述和用于检测病原性朊病毒的方法。 在一些实施方案中，抗体结合人朊蛋白的表位特征，其在其他物种的朊病毒蛋白中未发现。 在一些实施方案中，抗体不与牛，叙利亚金仓鼠，小鼠或大鼠朊病毒交叉反应。 该应用还包括用于检测含有朊病毒（PrP）蛋白质的样品中病原性朊病毒的构象依赖性免疫测定方法。 PrP蛋白可以以第一构象和第二构象存在，例如PrP和PrP Sc Sc，其中两种构象对于用于以下的抗体具有不同的结合亲和力 检测他们

公开(公告)号：US07202021B2

公开(公告)日：2007-04-10

申请号：US10273282

申请日：2002-10-18

申请人： Martin Vey ,  Weigand Lang ,  Albrecht Groener ,  Anne Bellon

发明人： Martin Vey ,  Weigand Lang ,  Albrecht Groener ,  Anne Bellon

IPC分类号： C12N5/12 ,  C12Q1/70 ,  C07K16/00 ,  C07H21/04 ,  C12N15/63

CPC分类号： G01N33/6896 ,  C07K16/18 ,  G01N2800/2828

摘要： This application includes description of antibodies for specifically detecting prions of human origin and methods for detecting pathogenic prions. In some embodiments, the antibodies bind to an epitope characteristic for a human prion protein which is not found in the prion proteins of other species. In some embodiments, the antibodies are not cross-reactive with cow, Syrian Gold hamster, mouse, or rat prions. The application also includes a conformation-dependent immunoassay method for detecting pathogenic prions in a sample containing a prion (PrP) protein. The PrP protein may be present in a first conformation and a second conformation, such as PrPC and PrPSc in which the two conformations have different binding affinity for the antibody used to detect them.

摘要翻译： 本申请包括用于特异性检测人源性朊病毒的抗体的描述和用于检测病原性朊病毒的方法。 在一些实施方案中，抗体结合在其他物种的朊病毒蛋白中未发现的人朊蛋白的表位特征。 在一些实施方案中，抗体不与牛，叙利亚金仓鼠，小鼠或大鼠朊病毒交叉反应。 该应用还包括用于检测含有朊病毒（PrP）蛋白质的样品中病原性朊病毒的构象依赖性免疫测定方法。 PrP蛋白可以以第一构象和第二构象存在，例如PrP和C pS Sc，其中两种构象对于用于以下的抗体具有不同的结合亲和力 检测他们

公开(公告)号：US07863006B2

公开(公告)日：2011-01-04

申请号：US11711020

申请日：2007-02-27

申请人： Martin Vey ,  Wiegang Lang ,  Albrecht Groener ,  Anne Bellon

发明人： Martin Vey ,  Wiegang Lang ,  Albrecht Groener ,  Anne Bellon

IPC分类号： G01N33/53 ,  G01N33/542 ,  G01N1/30 ,  G01N33/48 ,  C12P21/08 ,  C07K16/00

CPC分类号： G01N33/6896 ,  C07K16/18 ,  G01N2800/2828

摘要： This application includes description of antibodies for specifically detecting prions of human origin and methods for detecting pathogenic prions. In some embodiments, the antibodies bind to an epitope characteristic of a human prion protein which is not found in the prion proteins of other species. In some embodiments, the antibodies are not cross-reactive with cow, Syrian Gold hamster, mouse, or rat prions. The application also includes a conformation-dependent immunoassay method for detecting pathogenic prions in a sample containing a prion (PrP) protein. The PrP protein may be present in a first conformation and a second conformation, such as PrPc and PrPSc in which the two conformations have different binding affinity for the antibody used to detect them.

摘要翻译： 本申请包括用于特异性检测人源性朊病毒的抗体的描述和用于检测病原性朊病毒的方法。 在一些实施方案中，抗体结合人朊蛋白的表位特征，其在其他物种的朊病毒蛋白中未发现。 在一些实施方案中，抗体不与牛，叙利亚金仓鼠，小鼠或大鼠朊病毒交叉反应。 该应用还包括用于检测含有朊病毒（PrP）蛋白质的样品中病原性朊病毒的构象依赖性免疫测定方法。 PrP蛋白可以以第一构象和第二构象存在，例如PrPc和PrPSc，其中两种构象对于用于检测它们的抗体具有不同的结合亲和力。