公开(公告)号：US06472212B1

公开(公告)日：2002-10-29

申请号：US08820479

申请日：1997-03-18

申请人： Domenico Valerio ,  Victor Willem Van Beusechem

发明人： Domenico Valerio ,  Victor Willem Van Beusechem

IPC分类号： C12N1586

CPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/005 ,  C12N9/78 ,  C12N2740/13022 ,  C12N2740/13043 ,  C12N2740/13045 ,  C12N2810/6054 ,  C12N2810/855

摘要： A method is provided for genetically modifying primate bone marrow cells, comprising isolating bone marrow cells from a primate and, by means which enhance the local concentration of retroviral contacting the isolated bone marrow cells to cells that produce a recombinant amphotropic retrovirus with a genome based on a retroviral vector that contains the genetic information to be introduced into the bone marrow cells. Recombinant amphotropic retrovirus-producing cells, suitable for use in this method also are provided, as are genetically modified primate bone marrow cells with the capacity for regeneration in vivo.

摘要翻译： 提供了用于遗传修饰灵长类动物骨髓细胞的方法，其包括从灵长类动物分离骨髓细胞，并且通过增强逆转录病毒将分离的骨髓细胞与产生具有基因组的细胞接触的细胞的局部浓度的手段，所述基因组基于 包含要引入骨髓细胞的遗传信息的逆转录病毒载体。 还提供了适用于该方法的重组顺铂逆转录病毒产生细胞，以及具有体内再生能力的遗传修饰的灵长类动物骨髓细胞。

公开(公告)号：US07033834B2

公开(公告)日：2006-04-25

申请号：US09901836

申请日：2001-07-10

申请人： Domenico Valerio ,  Victor Willem Van Beusechem

发明人： Domenico Valerio ,  Victor Willem Van Beusechem

IPC分类号： A01N63/00 ,  A01N43/04 ,  C12N15/00 ,  C12N15/63 ,  C12N15/85

CPC分类号： C07K14/005 ,  A61K47/6898 ,  A61K47/6901 ,  A61K48/00 ,  B82Y5/00 ,  C07K14/70535 ,  C07K2319/32 ,  C12N7/00 ,  C12N15/86 ,  C12N15/87 ,  C12N2710/00088 ,  C12N2740/13043 ,  C12N2740/13045 ,  C12N2740/13052 ,  C12N2740/15022 ,  C12N2810/855 ,  C12N2810/859

摘要： A method for producing viral gene delivery vehicles which can be transferred to pre-selected cell types by using targeting conjugates. The gene delivery vehicles comprise: 1) the gene of interest; and 2) a viral capsid or envelope carrying a member of a specific binding pair, the counterpart of which is not directly associated with the surface of the target cell. These vehicles can be rendered unable to bind to their natural cell receptor. The targeting conjugates include the counterpart member of the specific binding pair, linked to a targeting moiety which is a cell-type specific ligand (or fragments thereof). The number of the specific binding pair present on the viral vehicles can be, for example, an immunoglobulin binding moiety (e.g., capable of binding to a Fc fragment, protein A, protein G, FcR or an anti-Ig antibody), or biotin, avidin or streptavidin. The virus' outer membrane or capsid may contain a substance which mediates entrance of the gene delivery vehicle into the target cell. Due to the specificity of the ligand, the binding pair's high affinity, and the gene delivery vehicle's inability to be targeted when used alone, the universality of the method for gene delivery, together with its high cell type selectively can be achieved by using various targeting conjugates.

公开(公告)号：US20110189234A1

公开(公告)日：2011-08-04

申请号：US12279114

申请日：2007-02-13

申请人： Victor Willem Van Beusechem ,  Frederik Hubertus Emanuel Schagen

发明人： Victor Willem Van Beusechem ,  Frederik Hubertus Emanuel Schagen

IPC分类号： A61K39/235 ,  C12N7/01 ,  C07H21/04 ,  C12N15/861 ,  A61P35/00 ,  A61P37/02

CPC分类号： C07K14/005 ,  C07K2319/73 ,  C07K2319/74 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10345 ,  C12N2720/12022 ,  C12N2720/12034 ,  C12N2810/6063

摘要： The present invention is concerned with means and methods for producing adenovirus particles comprising a chimeric adenovirus spike protein that essentially lacks a functional knob domain. One aspect of the invention is concerned with a method for producing adenovirus particles comprising providing cells that are permissive for adenovirus replication with an adenovirus vector, with nucleic acid encoding said chimeric adenovirus spike protein and with nucleic acid encoding at least one adenovirus E3 region protein or a functional part, derivative and/or analogue thereof, said method further comprising culturing said permissive cells to allow for at least one replication cycle of said adenovirus virus and harvesting said adenovirus particle.

摘要翻译： 本发明涉及用于产生包含嵌合腺病毒尖峰蛋白质的腺病毒颗粒的方法和方法，其基本上缺乏功能性旋钮结构域。 本发明的一个方面涉及生产腺病毒颗粒的方法，其包括提供允许腺病毒复制的细胞与腺病毒载体，编码所述嵌合腺病毒尖峰蛋白的核酸和编码至少一种腺病毒E3区蛋白的核酸或 其功能部分，衍生物和/或类似物，所述方法还包括培养所述允许细胞以允许所述腺病毒病毒的至少一个复制周期并收获所述腺病毒颗粒。