公开(公告)号：US06287569B1

公开(公告)日：2001-09-11

申请号：US09056105

申请日：1998-04-06

申请人： Thomas J. Kipps ,  Yunqi Wu

发明人： Thomas J. Kipps ,  Yunqi Wu

IPC分类号： A61K3912

CPC分类号： A61K39/0005 ,  A61K39/00 ,  A61K39/0011 ,  A61K48/00 ,  A61K2039/53 ,  A61K2039/57 ,  C12N2710/00088

摘要： Method for generating in a patient a cellular immune response to a target protein or portion thereof comprising the step of introducing into cells of the patient a vector containing a nucleotide sequence encoding a chimeric immunogen comprising a protein processing signal and the target protein or portion thereof, so that the chimeric immunogen is made within the cells and subsequently processed such that the target protein or portion thereof is presented to the patient's immune system so as to generate a cellular immune response.

摘要翻译： 在患者中产生对靶蛋白或其部分的细胞免疫应答的方法，其包括将含有编码包含蛋白处理信号和靶蛋白或其部分的嵌合免疫原的核苷酸序列的载体导入患者细胞的步骤， 使得嵌合免疫原在细胞内进行并随后进行处理，使得靶蛋白或其部分被呈递给患者的免疫系统以产生细胞免疫应答。

公开(公告)号：US08535682B2

公开(公告)日：2013-09-17

申请号：US12758500

申请日：2010-04-12

申请人： Sheena May Loosmore ,  Jean-Christophe Francis Audonnet ,  Jules Maarten Minke

发明人： Sheena May Loosmore ,  Jean-Christophe Francis Audonnet ,  Jules Maarten Minke

IPC分类号： A61K39/275 ,  A61K48/00 ,  C12N7/01 ,  C12N15/40 ,  C12N15/863 ,  C12N15/85

CPC分类号： A61K39/12 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/552 ,  A61K2039/55511 ,  A61K2039/55522 ,  A61K2039/55555 ,  C07K14/005 ,  C12N15/86 ,  C12N2710/00088 ,  C12N2710/24043 ,  C12N2770/24122 ,  C12N2770/24134 ,  C12N2770/24143 ,  C12N2799/023

摘要： An immunogenic or vaccine composition to induce an immune response or protective immune response against West Nile virus (WNV) in an animal susceptible to WNV. The composition includes a pharmaceutically or veterinarily acceptable vehicle or excipient, and a vector. The vector contains heterologous nucleic acid molecule(s), expresses in vivo in the animal WNV antigen, immunogen or epitope thereof, e.g., WNV E; WNV prM and E; WNV M and E; WNV prM, WNV M and E, WNV polyprotein prM-E, WNV polyprotein M-E, or WNV polyprotein prM-M-E. The composition can contain an adjuvant, such as carbomer. Methods for making and using such a composition, including prime-boost regimes and including as to differential diagnosis, are also contemplated.

摘要翻译： 用于在易感WNV的动物中诱导针对西尼罗病毒（WNV）的免疫应答或保护性免疫应答的免疫原性或疫苗组合物。 组合物包括药学上或兽医学上可接受的载体或赋形剂和载体。 载体含有异源核酸分子，在体内在动物WNV抗原中表达，其免疫原或其表位，例如WNV E; WNV prM和E; WNV M和E; WNV prM，WNV M和E，WNV多聚蛋白prM-E，WNV多聚蛋白M-E或WNV多聚蛋白prM-M-E。 组合物可以含有佐剂，如卡波姆。 还考虑制备和使用这种组合物的方法，包括初始 - 加强方案和包括鉴别诊断。

公开(公告)号：US07781208B2

公开(公告)日：2010-08-24

申请号：US11348661

申请日：2006-02-07

申请人： Domenico Valerio ,  Victor W. Van Beusechem

发明人： Domenico Valerio ,  Victor W. Van Beusechem

IPC分类号： C12N15/00 ,  C12N15/86

CPC分类号： C07K14/005 ,  A61K47/6898 ,  A61K47/6901 ,  A61K48/00 ,  B82Y5/00 ,  C07K14/70535 ,  C07K2319/32 ,  C12N7/00 ,  C12N15/86 ,  C12N15/87 ,  C12N2710/00088 ,  C12N2740/13043 ,  C12N2740/13045 ,  C12N2740/13052 ,  C12N2740/15022 ,  C12N2810/855 ,  C12N2810/859

摘要： A method for producing viral gene delivery vehicles which can be transferred to pre-selected cell types by using targeting conjugates. The gene delivery vehicles comprise: 1) the gene of interest; and 2) a viral capsid or envelope carrying a member of a specific binding pair, the counterpart of which is not directly associated with the surface of the target cell. These vehicles can be rendered unable to bind to their natural cell receptor. The targeting conjugates include the counterpart member of the specific binding pair, linked to a targeting moiety which is a cell-type specific ligand (or fragments thereof). The number of the specific binding pair present on the viral vehicles can be, for example, an immunoglobulin binding moiety (e.g., capable of binding to a Fc fragment, protein A, protein G, FcR or an anti-Ig antibody), or biotin, avidin or streptavidin. The virus' outer membrane or capsid may contain a substance which mediates entrance of the gene delivery vehicle into the target cell. Due to the specificity of the ligand, the binding pair's high affinity, and the gene delivery vehicle's inability to be targeted when used alone, the universality of the method for gene delivery, together with its high cell type selectively can be achieved by using various targeting conjugates.

摘要翻译： 用于产生病毒基因递送载体的方法，其可以通过使用靶向缀合物转移到预先选择的细胞类型。 基因递送载体包括：1）感兴趣的基因; 和2）携带特异性结合对的成员的病毒衣壳或信封，其对应物不与靶细胞的表面直接相关。 这些载体可能不能与其天然细胞受体结合。 靶向缀合物包括与作为细胞型特异性配体（或其片段）的靶向部分连接的特异性结合对的对应成员。 存在于病毒载体上的特异性结合对的数目可以是例如免疫球蛋白结合部分（例如，能够结合Fc片段，蛋白A，蛋白G，FcR或抗-Ig抗体）或生物素 ，抗生物素蛋白或链霉亲和素。 病毒的外膜或衣壳可以含有介导基因递送载体进入靶细胞的入口的物质。 由于配体的特异性，结合对的高亲和力和基因递送载体在单独使用时不能被靶向，所以基因递送方法的普及性及其高细胞类型选择性可以通过使用各种靶向 共轭物

公开(公告)号：US20070055049A1

公开(公告)日：2007-03-08

申请号：US10817970

申请日：2004-04-06

申请人： Howard Grey ,  Alessandro Sette ,  John Sidney ,  Scott Southwood ,  Ralph Kubo ,  Esteban Celis ,  Robert Chesnut ,  W. Kast

发明人： Howard Grey ,  Alessandro Sette ,  John Sidney ,  Scott Southwood ,  Ralph Kubo ,  Esteban Celis ,  Robert Chesnut ,  W. Kast

IPC分类号： C07K14/74

CPC分类号： A61K38/00 ,  A61K38/10 ,  A61K39/00 ,  A61K39/0011 ,  A61K39/02 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/5158 ,  A61K2039/55516 ,  A61K2039/55566 ,  A61K2039/572 ,  C07K7/06 ,  C07K14/70539 ,  C07K2319/01 ,  C07K2319/33 ,  C07K2319/40 ,  C12N2710/00088 ,  C12N2710/20034 ,  C12N2730/10134 ,  C12N2740/16034 ,  C12N2770/24234

摘要： The present invention provides the means and methods for selecting immunogenic peptides and the immunogenic peptide compositions capable of specifically binding glycoproteins encoded by HLA alleles and inducing T cell activation in T cells restricted by the allele. The peptides are useful to elicit an immune response against a desired antigen.

摘要翻译： 本发明提供了选择免疫原性肽的方法和方法以及能够特异性结合由HLA等位基因编码的糖蛋白并诱导由等位基因限制的T细胞中的T细胞活化的免疫原性肽组合物。 所述肽可用于引发针对所需抗原的免疫应答。

公开(公告)号：US20020115214A1

公开(公告)日：2002-08-22

申请号：US08592711

申请日：1996-01-26

发明人： CARL H. JUNE ,  CRAIG B. THOMPSON ,  GARY J. NABEL ,  GARY S. GRAY ,  PAUL D. RENNERT

IPC分类号： C12N005/06 ,  C12N005/16 ,  C12N005/08

CPC分类号： C12N5/0636 ,  A61K35/17 ,  A61K38/00 ,  A61K39/02 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/505 ,  A61K2039/507 ,  A61K2039/5158 ,  C07K14/705 ,  C07K14/70521 ,  C07K14/70532 ,  C07K16/00 ,  C07K16/2806 ,  C07K16/2809 ,  C07K16/2812 ,  C07K16/2815 ,  C07K16/2818 ,  C07K16/2833 ,  C07K16/2866 ,  C07K16/289 ,  C07K16/2896 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/54 ,  C07K2317/55 ,  C07K2317/73 ,  C07K2317/74 ,  C07K2319/00 ,  C07K2319/30 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2501/599 ,  C12N2710/00088 ,  C12N2740/16011

摘要： Methods for inducing a population of T cells to proliferate by activating the population of T cells and stimulating an accessory molecule on the surface of the T cells with a ligand which binds the accessory molecule are described. T cell proliferation occurs in the absence of exogenous growth factors or accessory cells. T cell activation is accomplished by stimulating the T cell receptor (TCR)/CD3 complex or the CD2 surface protein. To induce proliferation of an activated population T cells, an accessory molecule on the surface of the T cells, such as CD28, is stimulated with a ligand which binds the accessory molecule. The T cell population expanded by the method of the invention can be genetically transduced and used for immunotherapy or can be used in methods of diagnosis.

摘要翻译： 描述了通过激活T细胞群并用结合附属分子的配体刺激T细胞表面上的辅助分子来诱导T细胞群体增殖的方法。 T细胞增殖发生在没有外源生长因子或附属细胞的情况下。 通过刺激T细胞受体（TCR）/ CD3复合物或CD2表面蛋白来完成T细胞活化。 为了诱导活化的群体T细胞的增殖，T细胞表面上的辅助分子如CD28被与配合分子结合的配体刺激。 通过本发明的方法扩增的T细胞群体可以被遗传转导并用于免疫治疗，或可用于诊断方法。

公开(公告)号：US07740863B2

公开(公告)日：2010-06-22

申请号：US10714781

申请日：2003-11-17

申请人： Sheena May Loosmore ,  Jean-Christophe Francis Audonnet ,  Jules Maarten Minke

发明人： Sheena May Loosmore ,  Jean-Christophe Francis Audonnet ,  Jules Maarten Minke

IPC分类号： A61K39/12 ,  A61K39/275

CPC分类号： A61K39/12 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/552 ,  A61K2039/55511 ,  A61K2039/55522 ,  A61K2039/55555 ,  C07K14/005 ,  C12N15/86 ,  C12N2710/00088 ,  C12N2710/24043 ,  C12N2770/24122 ,  C12N2770/24134 ,  C12N2770/24143 ,  C12N2799/023

摘要： An immunogenic or vaccine composition to induce an immune response or protective immune response against West Nile virus (WNV) in an animal susceptible to WNV. The composition includes a pharmaceutically or veterinarily acceptable vehicle or excipient, and a vector. The vector contains heterologous nucleic acid molecule(s), expresses in vivo in the animal WNV antigen, immunogen or epitope thereof, e.g., WNV E; WNV prM and E; WNV M and E; WNV prM, WNV M and E, WNV polyprotein prM-E, WNV polyprotein M-E, or WNV polyprotein prM-M-E. The composition can contain an adjuvant, such as carbomer. Methods for making and using such a composition, including prime-boost regimes and including as to differential diagnosis, are also contemplated.

摘要翻译： 用于在易感WNV的动物中诱导针对西尼罗病毒（WNV）的免疫应答或保护性免疫应答的免疫原性或疫苗组合物。 组合物包括药学上或兽医学上可接受的载体或赋形剂和载体。 载体含有异源核酸分子，在体内在动物WNV抗原中表达，其免疫原或其表位，例如WNV E; WNV prM和E; WNV M和E; WNV prM，WNV M和E，WNV多聚蛋白prM-E，WNV多聚蛋白M-E或WNV多聚蛋白prM-M-E。 组合物可以含有佐剂，如卡波姆。 还考虑制备和使用这种组合物的方法，包括初始 - 加强方案和包括鉴别诊断。

公开(公告)号：US07723570B2

公开(公告)日：2010-05-25

申请号：US11249182

申请日：2005-10-12

申请人： Kenneth John Piller ,  Kenneth Lee Bost

发明人： Kenneth John Piller ,  Kenneth Lee Bost

IPC分类号： C12N15/82 ,  A01H5/00 ,  A61K39/085 ,  A61K47/00

CPC分类号： C12N15/8258 ,  A61K39/0005 ,  A61K39/02 ,  A61K39/0258 ,  A61K39/35 ,  A61K2039/517 ,  A61K2039/55544 ,  C12N2710/00088 ,  Y02A50/469 ,  Y02A50/474

摘要： The present invention relates to vaccines that are made in transgenic soybeans for use in humans, animals of agricultural importance, pets, and wildlife. These vaccines are used as vaccines against viral, bacterial, fungal, parasitic or prion related diseases, cancer antigens, toxins, and autologous or self proteins. The transgenic soybeans of the instant invention also can be used for inducing tolerance to allergens or tolerance to autoimmune antigens, wherein an individual shows hypersensitivity to said allergen or has developed autoimmunity to autologous or self proteins, respectively. The invention also relates to prophylatically treating individuals and/or populations prior to showing hypersensitivity to allergens. Other aspects of the invention include using the transgenic soybeans as an oral contraceptive, and the expression of protein adjuvants in transgenic soybeans.

摘要翻译： 本发明涉及用于人类的转基因大豆，具有农业重要性的动物，宠物和野生动物的疫苗。 这些疫苗用作抗病毒，细菌，真菌，寄生或朊病毒相关疾病，癌症抗原，毒素和自体或自身蛋白的疫苗。 本发明的转基因大豆还可用于诱导对变应原的耐受性或对自身免疫抗原的耐受性，其中个体对所述变应原显示过敏反应或分别对自体或自身蛋白产生自身免疫性。 本发明还涉及在对过敏原显示过敏之前预防性地治疗个体和/或群体。 本发明的其它方面包括使用转基因大豆作为口服避孕药，以及在转基因大豆中表达蛋白佐剂。

公开(公告)号：US20060134123A1

公开(公告)日：2006-06-22

申请号：US11348661

申请日：2006-02-07

申请人： Domenico Valerio ,  Victor Van Beusechem

发明人： Domenico Valerio ,  Victor Van Beusechem

IPC分类号： A61K48/00 ,  C07K16/46

CPC分类号： C07K14/005 ,  A61K47/6898 ,  A61K47/6901 ,  A61K48/00 ,  B82Y5/00 ,  C07K14/70535 ,  C07K2319/32 ,  C12N7/00 ,  C12N15/86 ,  C12N15/87 ,  C12N2710/00088 ,  C12N2740/13043 ,  C12N2740/13045 ,  C12N2740/13052 ,  C12N2740/15022 ,  C12N2810/855 ,  C12N2810/859

摘要： A method for producing viral gene delivery vehicles which can be transferred to pre-selected cell types by using targeting conjugates. The gene delivery vehicles comprise: 1) the gene of interest; and 2) a viral capsid or envelope carrying a member of a specific binding pair, the counterpart of which is not directly associated with the surface of the target cell. These vehicles can be rendered unable to bind to their natural cell receptor. The targeting conjugates include the counterpart member of the specific binding pair, linked to a targeting moiety which is a cell-type specific ligand (or fragments thereof). The number of the specific binding pair present on the viral vehicles can be, for example, an immunoglobulin binding moiety (e.g., capable of binding to a Fc fragment, protein A, protein G, FcR or an anti-Ig antibody), or biotin, avidin or streptavidin. The virus' outer membrane or capsid may contain a substance which mediates entrance of the gene delivery vehicle into the target cell. Due to the specificity of the ligand, the binding pair's high affinity, and the gene delivery vehicle's inability to be targeted when used alone, the universality of the method for gene delivery, together with its high cell type selectively can be achieved by using various targeting conjugates.

摘要翻译： 用于产生病毒基因递送载体的方法，其可以通过使用靶向缀合物转移到预先选择的细胞类型。 基因递送载体包括：1）感兴趣的基因; 和2）携带特异性结合对的成员的病毒衣壳或信封，其对应物不与靶细胞的表面直接相关。 这些载体可能不能与其天然细胞受体结合。 靶向缀合物包括与作为细胞型特异性配体（或其片段）的靶向部分连接的特异性结合对的对应成员。 存在于病毒载体上的特异性结合对的数目可以是例如免疫球蛋白结合部分（例如，能够结合Fc片段，蛋白A，蛋白G，FcR或抗-Ig抗体）或生物素 ，抗生物素蛋白或链霉亲和素。 病毒的外膜或衣壳可以含有介导基因递送载体进入靶细胞的入口的物质。 由于配体的特异性，结合对的高亲和力和基因递送载体在单独使用时不能被靶向，所以基因递送方法的普及性及其高细胞类型选择性可以通过使用各种靶向 共轭物

公开(公告)号：US07033834B2

公开(公告)日：2006-04-25

申请号：US09901836

申请日：2001-07-10

申请人： Domenico Valerio ,  Victor Willem Van Beusechem

发明人： Domenico Valerio ,  Victor Willem Van Beusechem

IPC分类号： A01N63/00 ,  A01N43/04 ,  C12N15/00 ,  C12N15/63 ,  C12N15/85

CPC分类号： C07K14/005 ,  A61K47/6898 ,  A61K47/6901 ,  A61K48/00 ,  B82Y5/00 ,  C07K14/70535 ,  C07K2319/32 ,  C12N7/00 ,  C12N15/86 ,  C12N15/87 ,  C12N2710/00088 ,  C12N2740/13043 ,  C12N2740/13045 ,  C12N2740/13052 ,  C12N2740/15022 ,  C12N2810/855 ,  C12N2810/859

摘要： A method for producing viral gene delivery vehicles which can be transferred to pre-selected cell types by using targeting conjugates. The gene delivery vehicles comprise: 1) the gene of interest; and 2) a viral capsid or envelope carrying a member of a specific binding pair, the counterpart of which is not directly associated with the surface of the target cell. These vehicles can be rendered unable to bind to their natural cell receptor. The targeting conjugates include the counterpart member of the specific binding pair, linked to a targeting moiety which is a cell-type specific ligand (or fragments thereof). The number of the specific binding pair present on the viral vehicles can be, for example, an immunoglobulin binding moiety (e.g., capable of binding to a Fc fragment, protein A, protein G, FcR or an anti-Ig antibody), or biotin, avidin or streptavidin. The virus' outer membrane or capsid may contain a substance which mediates entrance of the gene delivery vehicle into the target cell. Due to the specificity of the ligand, the binding pair's high affinity, and the gene delivery vehicle's inability to be targeted when used alone, the universality of the method for gene delivery, together with its high cell type selectively can be achieved by using various targeting conjugates.

公开(公告)号：US20010055791A1

公开(公告)日：2001-12-27

申请号：US09906326

申请日：2001-07-16

发明人： Allan S. Lau

IPC分类号： C12P021/02

CPC分类号： C12N9/1205 ,  C07K14/555 ,  C12N7/00 ,  C12N15/67 ,  C12N2710/00088 ,  C12N2710/16643 ,  C12P21/02 ,  C12Q1/18

摘要： Methods for enhancing the production of interferons in animal cell culture are described. These methods rely on the manipulation of the cellular levels of certain inducers of interferon production, in particular cellular levels of double-stranded-RNA-dependent kinase (dsRNA-PKR, or PKR). In cell cultures that overproduce PKR, interferon synthesis is induced to high levels, and significant amounts of interferon can be recovered without conventional induction of interferon by virus.

摘要翻译： 描述了用于增强动物细胞培养物中干扰素产生的方法。 这些方法依赖于干扰素产生的某些诱导物的细胞水平的操作，特别是双链RNA依赖性激酶（dsRNA-PKR或PKR）的细胞水平。 在过量产生PKR的细胞培养物中，干扰素合成被诱导为高水平，并且可以在不常规诱导病毒感染干扰素的情况下回收大量的干扰素。