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公开(公告)号：US08552174B2
公开(公告)日：2013-10-08
申请号：US11387269
申请日：2006-03-23
申请人： Douglas J. Dellinger , Zoltan Timar , Agnieszka Sierzchala , Geraldine Dellinger , Marvin H. Caruthers , Joel Myerson
发明人： Douglas J. Dellinger , Zoltan Timar , Agnieszka Sierzchala , Geraldine Dellinger , Marvin H. Caruthers , Joel Myerson
IPC分类号： C07H21/00
摘要： Methods of deprotecting polynucleotides are disclosed. One aspect of the method of deprotecting polynucleotides, among others, includes: providing a polynucleotide, wherein the polynucleotide includes at least one nucleotide monomer that has at least one protecting group selected from the following: a base having a protecting group, a 2′-hydroxyl protecting group, and a combination thereof, and deprotecting at least one of the protecting groups of the polynucleotide by introducing the polynucleotide to a solution including an α-effect nucleophile.
摘要翻译： 公开了脱保护多核苷酸的方法。 多核苷酸脱保护方法的一个方面包括：提供多核苷酸，其中所述多核苷酸包括至少一种具有至少一个选自以下的保护基的核苷酸单体：具有保护基的碱，2'- 羟基保护基团及其组合，并通过将多核苷酸引入到包含α-效应亲核试剂的溶液中来去保护多核苷酸的至少一个保护基团。
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2.发明授权Monomer compositions for the synthesis of RNA, methods of synthesis, and methods of deprotection 有权用于合成RNA的单体组合物，合成方法和脱保护方法公开(公告)号：US07759471B2
公开(公告)日：2010-07-20
申请号：US11388112
申请日：2006-03-23
申请人： Douglas J. Dellinger , Zoltan Timar , Agnieszka Sierzchala , Geraldine Dellinger , Marvin H. Caruthers
发明人： Douglas J. Dellinger , Zoltan Timar , Agnieszka Sierzchala , Geraldine Dellinger , Marvin H. Caruthers
IPC分类号： C07H19/00 , C07H19/067 , C07H19/167 , C07H21/02
摘要： Nucleotide monomers, polynucleotides, methods of making each, methods of deprotecting each, and the like are disclosed herein.
摘要翻译： 本文公开了核苷酸单体，多核苷酸，制备方法，各自的脱保护方法等。
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公开(公告)号：US20090082554A1
公开(公告)日：2009-03-26
申请号：US11903821
申请日：2007-09-24
摘要： In various embodiments of the invention, novel compositions having a polynucleotide bound to a substrate via a cleavable linker are provided, and methods of cleaving a polynucleotide from a substrate are provided.
摘要翻译： 在本发明的各种实施方案中，提供了具有通过可切割连接子与底物结合的多核苷酸的新型组合物，并提供了从底物切割多核苷酸的方法。
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公开(公告)号：US07790387B2
公开(公告)日：2010-09-07
申请号：US11903821
申请日：2007-09-24
摘要： In various embodiments of the invention, novel compositions having a polynucleotide bound to a substrate via a cleavable linker are provided, and methods of cleaving a polynucleotide from a substrate are provided.
摘要翻译： 在本发明的各种实施方案中，提供了具有通过可切割连接子与底物结合的多核苷酸的新型组合物，并提供了从底物切割多核苷酸的方法。
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5.发明授权Monomer compositions for the synthesis of polynucleotides, methods of synthesis, and methods of deprotection 有权用于合成多核苷酸的单体组合物，合成方法和脱保护方法公开(公告)号：US08202985B2
公开(公告)日：2012-06-19
申请号：US11387388
申请日：2006-03-23
申请人： Douglas J. Dellinger , Zoltan Timar , Agnieszka Sierzchala , Geraldino Dellinger , Marvin H. Caruthers
发明人： Douglas J. Dellinger , Zoltan Timar , Agnieszka Sierzchala , Geraldino Dellinger , Marvin H. Caruthers
IPC分类号： C07H19/067 , C07H19/073 , C07H19/167 , C07H19/173 , C07H21/02 , C07H21/04
摘要： Nucleotide monomers, polynucleotides, methods of making each, and methods of deprotecting each, are disclosed. An embodiment of the nucleotide monomer, among others, includes a nucleotide monomer having a heterobase protecting group selected from structures I through III as described herein. An embodiment of the polynucleotide, among others, includes a plurality of nucleotide moieties having a heterobase protecting group selected from one of structures I through III as described herein.
摘要翻译： 公开了核苷酸单体，多核苷酸，制备方法，以及每种脱保护方法。 核苷酸单体的一个实施方案包括具有选自如本文所述的结构I至III的杂基保护基团的核苷酸单体。 多核苷酸的一个实施方案包括多个具有选自如本文所述的结构I至III之一的异源保护基团的核苷酸部分。
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公开(公告)号：US07368550B2
公开(公告)日：2008-05-06
申请号：US11388339
申请日：2006-03-23
IPC分类号： C07H21/00
摘要： Compounds having a phosphorus group structure of: wherein: R is alkyl, modified lower alkyl; and Ri and Ri are each independently H, alkyl, modified alkyl, or aryl; are provided. Also provided are polynucleotide compositions that include these compounds and methods of using the compounds in synthesis of the same.
摘要翻译： 具有磷基结构的化合物：其中：R是烷基，改性的低级烷基; 和R i和R ii各自独立地为H，烷基，改性烷基或芳基; 被提供。 还提供了包括这些化合物的多核苷酸组合物和在其合成中使用该化合物的方法。
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公开(公告)号：US20120289691A1
公开(公告)日：2012-11-15
申请号：US13531191
申请日：2012-06-22
申请人： Douglas J. Dellinger , Joel Myerson , Agnieszka Sierzchala , Geraldine F. Dellinger , Zoltan Timar
发明人： Douglas J. Dellinger , Joel Myerson , Agnieszka Sierzchala , Geraldine F. Dellinger , Zoltan Timar
IPC分类号： C07H1/00
CPC分类号： C07H19/067 , A61K31/712 , A61K31/7125 , C07H1/00 , C07H13/12 , C07H19/00 , C07H19/167 , C07H21/00 , C07H21/02 , C07H21/04 , C07H23/00 , Y02P20/55
摘要： A method of deprotecting a solid support bound polynucleotide includes the step of contacting the polynucleotide with a composition comprising a diamine under conditions sufficient to deprotect the 2′-protected ribonucleotide residue. The solid support bound polynucleotide has at least one 2′-protected ribonucleotide residue, which has the following structure: wherein BP is a protected or unprotected heterocycle; R12 is a protecting group selected from a hydrocarbyl, a substituted hydrocarbyl, an aryl, and a substituted aryl; X is O or S; and PG is a thionocarbamate protecting group.
摘要翻译： 使固体支持物结合的多核苷酸去保护的方法包括在足以使2'-保护的核糖核苷酸残基脱保护的条件下使多核苷酸与包含二胺的组合物接触的步骤。 固体支持结合的多核苷酸具有至少一个2'-保护的核糖核苷酸残基，其具有以下结构：其中BP是被保护或未被保护的杂环; R 12是选自烃基，取代的烃基，芳基和取代的芳基的保护基; X是O或S; PG是硫代氨基甲酸酯保护基。
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8.发明申请公开(公告)号：US20100076183A1
公开(公告)日：2010-03-25
申请号：US12466326
申请日：2009-05-14
申请人： Douglas J. Dellinger , Joel Myerson , Agnieszka Sierzchala , Geraldine F. Dellinger , Zoltan Timar
发明人： Douglas J. Dellinger , Joel Myerson , Agnieszka Sierzchala , Geraldine F. Dellinger , Zoltan Timar
IPC分类号： C07H17/00 , C07H21/00 , C07C211/10
CPC分类号： C07H19/067 , A61K31/712 , A61K31/7125 , C07H1/00 , C07H13/12 , C07H19/00 , C07H19/167 , C07H21/00 , C07H21/02 , C07H21/04 , C07H23/00 , Y02P20/55
摘要： A nucleoside monomer that is protected by a thionocarbamate protecting group is provided, as well as a method for making a polynucleotide that uses the same. Also provided is a polynucleotide synthesis method that employs a diamine to deprotect a protected polynucleotide.
摘要翻译： 提供了由硫羰氨基保护基保护的核苷单体，以及制备使用该核苷单体的多核苷酸的方法。 还提供了使用二胺去保护受保护的多核苷酸的多核苷酸合成方法。
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公开(公告)号：US20070100137A1
公开(公告)日：2007-05-03
申请号：US11387269
申请日：2006-03-23
申请人： Douglas Dellinger , Zoltan Timar , Agnieszka Sierzchala , Geraldine Dellinger , Marvin Caruthers , Joel Myerson
发明人： Douglas Dellinger , Zoltan Timar , Agnieszka Sierzchala , Geraldine Dellinger , Marvin Caruthers , Joel Myerson
IPC分类号： C07H21/04
摘要： Methods of deprotecting polynucleotides are disclosed.
摘要翻译： 公开了脱保护多核苷酸的方法。
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公开(公告)号：US20070224602A1
公开(公告)日：2007-09-27
申请号：US11389326
申请日：2006-03-23
CPC分类号： C07H21/04
摘要： In various embodiments of the invention, novel compositions having a polynucleotide bound to a substrate via a cleavable linker are provided, and methods of cleaving a polynucleotide from a substrate are provided.
摘要翻译： 在本发明的各种实施方案中，提供了具有通过可切割连接子与底物结合的多核苷酸的新型组合物，并提供了从底物切割多核苷酸的方法。
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