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    Membrane-Translocating Peptides
    4.
    发明申请
    Membrane-Translocating Peptides 有权
    膜 - 位移肽

    公开(公告)号：US20080287311A1

    公开(公告)日：2008-11-20

    申请号：US11996496

    申请日：2006-07-24

    申请人： David Coomber ,  Kevin Fitzgerald ,  Duncan McGregor ,  Chris Ullman ,  Tomas Leanderson

    发明人： David Coomber ,  Kevin Fitzgerald ,  Duncan McGregor ,  Chris Ullman ,  Tomas Leanderson

    IPC分类号： C40B30/04 ,  C07K14/00 ,  C07H21/00 ,  C12N5/06

    CPC分类号： C12N15/1075

    摘要： A method is provided for selecting membrane-translocating peptides (MTPs) from a peptide display library that are capable of crossing or penetrating a lipid membrane. A plurality of nucleic acid constructs that encode displayed peptides are expressed, resulting in the formation of a plurality of nucleic acid-peptide complexes, each complex comprising at least one displayed peptide associated with the corresponding nucleic acid construct encoding the displayed peptide; the complexes are exposed to a population of membrane-encapsulated compartments, allowing a translocating reaction to occur; complexes that remain unassociated with the membrane are removed; optionally complexes that are associated with the membrane are removed; and internalised nucleic acid-peptide complexes are recovered. The membrane-encapsulated compartments may be artificial vesicles such as liposomes, or populations of one or more cell types.

    摘要翻译： 提供了从能够穿透或穿透脂质膜的肽展示文库中选择膜转位肽（MTP）的方法。 表达编码显示的肽的多个核酸构建体，导致形成多个核酸 - 肽复合物，每个复合物包含与编码所显示肽的相应核酸构建体相关联的至少一个显示的肽; 复合物暴露于膜包封的隔室群，允许发生易位反应; 与膜保持不相关的复合物被去除; 去除与膜相关的任选复合物; 并回收内化的核酸 - 肽复合物。 膜包封的隔室可以是人造囊泡，例如脂质体或一种或多种细胞类型的群体。

    Membrane-translocating peptides
    5.
    发明授权
    Membrane-translocating peptides 有权
    膜易位肽

    公开(公告)号：US08557744B2

    公开(公告)日：2013-10-15

    申请号：US11996496

    申请日：2006-07-24

    申请人： David Coomber ,  Kevin Fitzgerald ,  Duncan McGregor ,  Chris Ullman ,  Tomas Leanderson

    发明人： David Coomber ,  Kevin Fitzgerald ,  Duncan McGregor ,  Chris Ullman ,  Tomas Leanderson

    IPC分类号： C40B30/04 ,  C12Q1/70

    CPC分类号： C12N15/1075

    摘要： A method is selects membrane-translocating peptides (MTPs) from a peptide display library that are capable of crossing or penetrating a lipid membrane. A plurality of nucleic acid constructs that encode displayed peptides are expressed, resulting in the formation of a plurality of nucleic acid-peptide complexes, each complex comprising at least one displayed peptide associated with the corresponding nucleic acid construct encoding the displayed peptide; the complexes are exposed to a population of membrane-encapsulated compartments, allowing a translocating reaction to occur; complexes that remain unassociated with the membrane are removed; optionally complexes that are associated with the membrane are removed; and internalized nucleic acid-peptide complexes are recovered. The membrane-encapsulated compartments may be artificial vesicles such as liposomes, or populations of one or more cell types.

    摘要翻译： 一种方法是从能够穿透或穿透脂质膜的肽展示文库中选择膜 - 位移肽（MTP）。 表达编码显示的肽的多个核酸构建体，导致形成多个核酸 - 肽复合物，每个复合物包含与编码所显示肽的相应核酸构建体相关联的至少一个显示的肽; 复合物暴露于膜包封的隔室群，允许发生易位反应; 与膜保持不相关的复合物被去除; 去除与膜相关的任选复合物; 并回收内化的核酸 - 肽复合物。 膜包封的隔室可以是人造囊泡，例如脂质体或一种或多种细胞类型的群体。

    SCAFFOLD PEPTIDES
    6.
    发明申请
    SCAFFOLD PEPTIDES 审中-公开

    公开(公告)号：US20140005126A1

    公开(公告)日：2014-01-02

    申请号：US13814536

    申请日：2011-08-08

    申请人： Chris Ullman ,  Agnes Jaulent ,  Seema Patel ,  Pascale Mathonet

    发明人： Chris Ullman ,  Agnes Jaulent ,  Seema Patel ,  Pascale Mathonet

    IPC分类号： C07K14/47

    CPC分类号： C07K14/47 ,  C12N15/1044

    摘要： A naive WW domain peptide library derived from a WW domain peptide sequence which has been diversified by changing the amino acid sequence at one or more positions is provided. The naive WW domain peptide library may be derived from a GroupIV WW domain peptide. Methods for making the naive WW domain peptide library and methods for selected a modified WW domain peptide that binds a target ligand using the naive WW domain peptide library are also provided. Also disclosed are modified WW domain peptides that bind desired target ligands, pharmaceutical compositions comprising such peptides, and uses for such peptides. The modified WW domain peptides have altered, improved or different, target ligand binding characteristics to those of the unmodified WW domain peptides from which they are derived.

    摘要翻译： 提供了源自通过改变一个或多个位置上的氨基酸序列而多样化的WW结构域肽序列的初始WW结构域肽文库。 天真的WW结构域肽文库可以衍生自组IVWN结构域肽。 还提供了使用初始WW结构域肽文库制备初始WW结构域肽文库的方法和选择使用初始WW结构域肽文库结合靶配体的修饰的WW结构域肽的方法。 还公开了结合所需靶配体的修饰的WW结构域肽，包含这些肽的药物组合物，以及这些肽的用途。 修饰的WW结构域肽具有改变的，改进的或不同的靶配体结合特征，与未衍生的来自其的未修饰的WW结构域肽的配体结合特征。