公开(公告)号：US07842476B2

公开(公告)日：2010-11-30

申请号：US10526479

申请日：2003-09-05

申请人： Duncan McGregor ,  Richard Odegrip ,  Kevin Fizgerald ,  Rosemarie Hederer ,  Bill Eldridge ,  Chris Ullman ,  Philip Kuhlman ,  David Coomber

发明人： Duncan McGregor ,  Richard Odegrip ,  Kevin Fizgerald ,  Rosemarie Hederer ,  Bill Eldridge ,  Chris Ullman ,  Philip Kuhlman ,  David Coomber

IPC分类号： C12P21/06 ,  C12P19/34 ,  C12Q1/68 ,  C12N15/00

CPC分类号： C12N15/1075 ,  C07K2319/80

摘要： The invention provides a method for making in vitro peptide expression libraries, and for the isolation of nucleotide sequences encoding peptides of interest, wherein the peptides or proteins are specifically associated with the DNA encoding them through non-covalent protein:DNA binding. The method describes ways of making the library itself, DNA molecules encoding the library and uses of the expression library.

摘要翻译： 本发明提供了制备体外肽表达文库以及分离编码目的肽的核苷酸序列的方法，其中肽或蛋白质通过非共价蛋白质DNA结合与编码它们的DNA特异性结合。 该方法描述了制作库本身的方法，编码该库的DNA分子和表达库的用途。

公开(公告)号：US20060035232A1

公开(公告)日：2006-02-16

申请号：US10526479

申请日：2003-09-05

申请人： Duncan McGregor ,  Richard Odegrip ,  Kevin Fizgerald ,  Rosemarie Hederer ,  Bill Eldridge ,  Chris Ullman ,  Philip Kuhlman ,  David Coomber

发明人： Duncan McGregor ,  Richard Odegrip ,  Kevin Fizgerald ,  Rosemarie Hederer ,  Bill Eldridge ,  Chris Ullman ,  Philip Kuhlman ,  David Coomber

IPC分类号： C40B40/08 ,  C12P19/34

CPC分类号： C12N15/1075 ,  C07K2319/80

摘要： The invention provides a method for making in vitro peptide expression libraries, and for the isolation of nucleotide sequences encoding peptides of interest, wherein the peptides or proteins are specifically associated with the DNA encoding them through non-covalent protein:DNA binding. The method describes ways of making the library itself, DNA molecules encoding the library and uses of the expression library.

摘要翻译： 本发明提供了制备体外肽表达文库以及分离编码目的肽的核苷酸序列的方法，其中肽或蛋白质通过非共价蛋白质DNA结合与编码它们的DNA特异性结合。 该方法描述了制作库本身的方法，编码该库的DNA分子和表达库的用途。

公开(公告)号：US08679781B2

公开(公告)日：2014-03-25

申请号：US12909420

申请日：2010-10-21

申请人： Duncan McGregor ,  Richard Odegrip ,  Kevin Fitzgerald ,  Rosemarie Hederer ,  Bill Eldridge ,  Chris Ullman ,  Philip Kuhlman ,  David Coomber

发明人： Duncan McGregor ,  Richard Odegrip ,  Kevin Fitzgerald ,  Rosemarie Hederer ,  Bill Eldridge ,  Chris Ullman ,  Philip Kuhlman ,  David Coomber

IPC分类号： C12P21/06 ,  C12P19/34 ,  C12Q1/68 ,  C12N15/00

CPC分类号： C12N15/1075 ,  C07K2319/80

摘要： The invention provides a method for making in vitro peptide expression libraries, and for the isolation of nucleotide sequences encoding peptides of interest, wherein the peptides or proteins are specifically associated with the DNA encoding them through non-covalent protein:DNA binding. The method describes ways of making the library itself, DNA molecules encoding the library and uses of the expression library.

摘要翻译： 本发明提供了制备体外肽表达文库以及分离编码目的肽的核苷酸序列的方法，其中肽或蛋白质通过非共价蛋白质DNA结合与编码它们的DNA特异性结合。 该方法描述了制作库本身的方法，编码该库的DNA分子和表达库的用途。

公开(公告)号：US20080287311A1

公开(公告)日：2008-11-20

申请号：US11996496

申请日：2006-07-24

申请人： David Coomber ,  Kevin Fitzgerald ,  Duncan McGregor ,  Chris Ullman ,  Tomas Leanderson

发明人： David Coomber ,  Kevin Fitzgerald ,  Duncan McGregor ,  Chris Ullman ,  Tomas Leanderson

IPC分类号： C40B30/04 ,  C07K14/00 ,  C07H21/00 ,  C12N5/06

CPC分类号： C12N15/1075

摘要： A method is provided for selecting membrane-translocating peptides (MTPs) from a peptide display library that are capable of crossing or penetrating a lipid membrane. A plurality of nucleic acid constructs that encode displayed peptides are expressed, resulting in the formation of a plurality of nucleic acid-peptide complexes, each complex comprising at least one displayed peptide associated with the corresponding nucleic acid construct encoding the displayed peptide; the complexes are exposed to a population of membrane-encapsulated compartments, allowing a translocating reaction to occur; complexes that remain unassociated with the membrane are removed; optionally complexes that are associated with the membrane are removed; and internalised nucleic acid-peptide complexes are recovered. The membrane-encapsulated compartments may be artificial vesicles such as liposomes, or populations of one or more cell types.

摘要翻译： 提供了从能够穿透或穿透脂质膜的肽展示文库中选择膜转位肽（MTP）的方法。 表达编码显示的肽的多个核酸构建体，导致形成多个核酸 - 肽复合物，每个复合物包含与编码所显示肽的相应核酸构建体相关联的至少一个显示的肽; 复合物暴露于膜包封的隔室群，允许发生易位反应; 与膜保持不相关的复合物被去除; 去除与膜相关的任选复合物; 并回收内化的核酸 - 肽复合物。 膜包封的隔室可以是人造囊泡，例如脂质体或一种或多种细胞类型的群体。

公开(公告)号：US08557744B2

公开(公告)日：2013-10-15

申请号：US11996496

申请日：2006-07-24

申请人： David Coomber ,  Kevin Fitzgerald ,  Duncan McGregor ,  Chris Ullman ,  Tomas Leanderson

发明人： David Coomber ,  Kevin Fitzgerald ,  Duncan McGregor ,  Chris Ullman ,  Tomas Leanderson

IPC分类号： C40B30/04 ,  C12Q1/70

CPC分类号： C12N15/1075

摘要： A method is selects membrane-translocating peptides (MTPs) from a peptide display library that are capable of crossing or penetrating a lipid membrane. A plurality of nucleic acid constructs that encode displayed peptides are expressed, resulting in the formation of a plurality of nucleic acid-peptide complexes, each complex comprising at least one displayed peptide associated with the corresponding nucleic acid construct encoding the displayed peptide; the complexes are exposed to a population of membrane-encapsulated compartments, allowing a translocating reaction to occur; complexes that remain unassociated with the membrane are removed; optionally complexes that are associated with the membrane are removed; and internalized nucleic acid-peptide complexes are recovered. The membrane-encapsulated compartments may be artificial vesicles such as liposomes, or populations of one or more cell types.

摘要翻译： 一种方法是从能够穿透或穿透脂质膜的肽展示文库中选择膜 - 位移肽（MTP）。 表达编码显示的肽的多个核酸构建体，导致形成多个核酸 - 肽复合物，每个复合物包含与编码所显示肽的相应核酸构建体相关联的至少一个显示的肽; 复合物暴露于膜包封的隔室群，允许发生易位反应; 与膜保持不相关的复合物被去除; 去除与膜相关的任选复合物; 并回收内化的核酸 - 肽复合物。 膜包封的隔室可以是人造囊泡，例如脂质体或一种或多种细胞类型的群体。

公开(公告)号：US20070128672A1

公开(公告)日：2007-06-07

申请号：US11421368

申请日：2006-05-31

申请人： Robyn Ward ,  David Coomber

发明人： Robyn Ward ,  David Coomber

IPC分类号： G01N33/574 ,  C07H21/04 ,  C12P21/06 ,  C07K14/82 ,  C07K16/30

CPC分类号： G01N33/57484 ,  A61K48/00 ,  A61K2039/505 ,  C07K16/18 ,  C07K2317/21 ,  C07K2317/55 ,  C12Q1/6883 ,  C12Q2600/156

摘要： The present invention relates to nucleotide sequences which encode polypeptides of antibodies against the p53 protein in vertebrates, and to the polypeptides and antibodies (or fragments thereof) encoded by those nucleotide sequences. The invention also relates to nucleotide sequences and polypeptide sequences for use in the development of diagnostic and therapeutic compositions, and to methods of using those diagnostic and therpeutic compositions in the diagnosis and treatment of cancer, rheumatoid arthritis and other disease states which exhibit abnormalities of p53.

摘要翻译： 本发明涉及编码脊椎动物中针对p53蛋白的抗体多肽的核苷酸序列，以及由这些核苷酸序列编码的多肽和抗体（或其片段）。 本发明还涉及用于开发诊断和治疗组合物的核苷酸序列和多肽序列，以及将这些诊断和治疗组合物用于诊断和治疗癌症，类风湿性关节炎和其它呈现p53异常的疾病状态的方法 。

公开(公告)号：US08948592B2

公开(公告)日：2015-02-03

申请号：US12714600

申请日：2010-03-01

申请人： Thomas Haslam ,  David Coomber ,  Daniel Doliska ,  Michael Scott Pegrum ,  Marc-Antoine Boutin ,  Brian Sungwan Kim

发明人： Thomas Haslam ,  David Coomber ,  Daniel Doliska ,  Michael Scott Pegrum ,  Marc-Antoine Boutin ,  Brian Sungwan Kim

IPC分类号： H04J14/02

CPC分类号： H04Q11/0005 ,  H04J14/0201 ,  H04J14/0204 ,  H04J14/0206 ,  H04J14/0212 ,  H04Q2011/0037

摘要： The invention relates to a method for auto-configuring a wavelength selective switch (WSS) device having an output port and a plurality of input ports and coupled to a WSS controller. When connected to a WDM optical network, the WSS controller is programmed to utilize one or more optical channel monitors (OCM) coupled to the input and/or output ports to detect which of the wavelengths are present at the input ports. Wavelengths that are not detected on any input port are blocked by the WSS. Any wavelength detected as present at one and only one input port is switched by the WSS to the output port. If a wavelength is detected at two or more input ports, it is either blocked by the WSS at each of the input ports until user intervention, or is blocked at all but one of the input ports as defined by assigned port priorities.

摘要翻译： 本发明涉及一种用于自动配置具有输出端口和多个输入端口并耦合到WSS控制器的波长选择开关（WSS）设备的方法。 当连接到WDM光网络时，WSS控制器被编程为利用耦合到输入和/或输出端口的一个或多个光通道监视器（OCM）来检测在输入端口处存在哪些波长。 在任何输入端口上未检测到的波长被WSS阻止。 在一个且仅一个输入端口上检测到的任何波长被WSS切换到输出端口。 如果在两个或更多个输入端口检测到波长，则在每个输入端口由WSS阻塞，直到用户干预，或者由分配的端口优先级定义的除输入端口之外的所有输入端口都被阻塞。

公开(公告)号：US20100221004A1

公开(公告)日：2010-09-02

申请号：US12714600

申请日：2010-03-01

申请人： Thomas Haslam ,  David Coomber ,  Daniel Doliska ,  Michael Scott Pegrum ,  Marc-Antoine Boutin ,  Brian Sungwan Kim

发明人： Thomas Haslam ,  David Coomber ,  Daniel Doliska ,  Michael Scott Pegrum ,  Marc-Antoine Boutin ,  Brian Sungwan Kim

IPC分类号： H04J14/00

CPC分类号： H04Q11/0005 ,  H04J14/0201 ,  H04J14/0204 ,  H04J14/0206 ,  H04J14/0212 ,  H04Q2011/0037

摘要： The invention relates to a method for auto-configuring a wavelength selective switch (WSS) device having an output port and a plurality of input ports and coupled to a WSS controller. When connected to a WDM optical network, the WSS controller is programmed to utilize one or more optical channel monitors (OCM) coupled to the input and/or output ports to detect which of the wavelengths are present at the input ports. Wavelengths that are not detected on any input port are blocked by the WSS. Any wavelength detected as present at one and only one input port is switched by the WSS to the output port. If a wavelength is detected at two or more input ports, it is either blocked by the WSS at each of the input ports until user intervention, or is blocked at all but one of the input ports as defined by assigned port priorities.

摘要翻译： 本发明涉及一种用于自动配置具有输出端口和多个输入端口并耦合到WSS控制器的波长选择开关（WSS）设备的方法。 当连接到WDM光网络时，WSS控制器被编程为利用耦合到输入和/或输出端口的一个或多个光通道监视器（OCM）来检测在输入端口处存在哪些波长。 在任何输入端口上未检测到的波长被WSS阻止。 在一个且仅一个输入端口上检测到的任何波长被WSS切换到输出端口。 如果在两个或更多个输入端口检测到波长，则在每个输入端口由WSS阻塞，直到用户干预，或者由分配的端口优先级定义的除输入端口之外的所有输入端口都被阻塞。