公开(公告)号：US09127283B2

公开(公告)日：2015-09-08

申请号：US13303652

申请日：2011-11-23

申请人： Dwayne Bisgrove ,  Hiroaki Sagawa

发明人： Dwayne Bisgrove ,  Hiroaki Sagawa

IPC分类号： C12N15/09 ,  C12N15/31 ,  C12N15/63 ,  C07K14/47 ,  C12N15/82

CPC分类号： C12N15/635 ,  C07K14/4703 ,  C07K14/4705 ,  C07K2319/10 ,  C07K2319/80 ,  C07K2319/81 ,  C12N15/8217 ,  C12N15/8238

摘要： Aspects of the invention include inducible expression systems in which a transcription modulator having a distributed protein transduction domain is employed. Aspects of the invention further include methods of using the systems to induce expression of a coding sequence, as well as kits that find use in practicing methods of the invention. The systems, components thereof, methods and kits find use in a variety of different applications.

摘要翻译： 本发明的方面包括其中使用具有分布的蛋白质转导结构域的转录调节物的诱导型表达系统。 本发明的方面还包括使用该系统诱导编码序列表达的方法，以及在实践本发明方法中使用的试剂盒。 其系统，组件，方法和套件可用于各种不同的应用。

公开(公告)号：US20120190107A1

公开(公告)日：2012-07-26

申请号：US13358450

申请日：2012-01-25

申请人： Dwayne Bisgrove ,  Hiroaki Sagawa

发明人： Dwayne Bisgrove ,  Hiroaki Sagawa

IPC分类号： C12N5/071 ,  C12N5/07 ,  C12N1/21 ,  C12N1/14 ,  C12N1/16 ,  C12N15/63 ,  C12N5/04

CPC分类号： C07K14/47 ,  C07K2319/10 ,  C12N15/88

摘要： Methods of enhanced protein transduction are provided. Aspects of the methods include contacting a cell with a transduction protein, where the transduction protein includes both a protein-of-interest domain and a protein transduction domain, and a nucleic acid transfection agent. Also provided are systems and kits that find use in practicing methods according to embodiments of the invention. The methods, systems and kits find use in a variety of different applications.

摘要翻译： 提供增强蛋白转导的方法。 方法的方面包括使细胞与转导蛋白接触，其中转导蛋白包括感兴趣的蛋白质结构域和蛋白质转导结构域，以及核酸转染试剂。 还提供了根据本发明的实施例的用于实践方法的系统和试剂盒。 方法，系统和试剂盒可用于各种不同的应用。

公开(公告)号：US09945850B2

公开(公告)日：2018-04-17

申请号：US13208267

申请日：2011-08-11

申请人： Dwayne Bisgrove ,  Hiroaki Sagawa

发明人： Dwayne Bisgrove ,  Hiroaki Sagawa

IPC分类号： A61K38/00 ,  G01N33/558 ,  C07K14/005

CPC分类号： G01N33/558 ,  A61K38/00 ,  C07K14/005

摘要： Methods of determining whether a non-diagnostic analyte is present in a non-diagnostic sample are provided. Aspects of the methods include applying a non-diagnostic sample to a sample receiving region of a lateral flow assay device and reading a detection region to determine whether a non-diagnostic analyte is present in the non-diagnostic sample. Also provided are kits that find use in practicing methods of the invention.

公开(公告)号：US20120040336A1

公开(公告)日：2012-02-16

申请号：US13208267

申请日：2011-08-11

申请人： Dwayne Bisgrove ,  Hiroaki Sagawa

发明人： Dwayne Bisgrove ,  Hiroaki Sagawa

IPC分类号： C12Q1/70 ,  G01N33/53 ,  C12M1/34

CPC分类号： G01N33/558 ,  A61K38/00 ,  C07K14/005

摘要： Methods of determining whether a non-diagnostic analyte is present in a non-diagnostic sample are provided. Aspects of the methods include applying a non-diagnostic sample to a sample receiving region of a lateral flow assay device and reading a detection region to determine whether a non-diagnostic analyte is present in the non-diagnostic sample. Also provided are kits that find use in practicing methods of the invention.

摘要翻译： 提供了确定非诊断分析物是否存在于非诊断样品中的方法。 方法的方面包括将非诊断样品应用于侧流测定装置的样品接收区域并读取检测区域以确定非诊断样品中是否存在非诊断分析物。 还提供了用于本发明的实践方法的试剂盒。