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1.发明授权公开(公告)号：US08911983B2
公开(公告)日：2014-12-16
申请号：US13711646
申请日：2012-12-12
发明人： Perry G G Caimi , Min Qi , Luan Tao , Paul V Viitanen , Jianjun Yang
CPC分类号： C12N15/74 , C12N9/1258 , C12N9/90 , C12N9/92 , C12P7/065 , C12P7/10 , C12Y207/07008 , C12Y501/03001 , C12Y503/01005 , C12Y503/01006 , Y02E50/16 , Y02E50/17
摘要： The endogenous pnp gene encoding polynucleotide phosphorylase in the Zymomonas genome was identified as a target for modification to provide improved xylose utilizing cells for ethanol production. The cells are in addition genetically modified to have increased expression of ribose-5-phosphate isomerase (RPI) activity, as compared to cells without this genetic modification, and are not limited in xylose isomerase activity in the absence of the pnp modification.
摘要翻译： 在酵母单位基因组中编码多核苷酸磷酸化酶的内源性pnp基因被鉴定为用于修饰以提供用于乙醇生产的细胞的改良木糖的靶标。 与没有这种遗传修饰的细胞相比，细胞另外进行遗传修饰以具有增加的核糖-5-磷酸异构酶（RPI）活性的表达，并且在没有pnp修饰的情况下不限制于木糖异构酶活性。
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2.发明申请GENE INACTIVATION ALLOWING IMMEDIATE GROWTH ON XYLOSE MEDIUM BY ENGINEERED ZYMOMONAS 审中-公开基因失活在工程化的ZYMOMONAS上允许立即生长在XYLOSE中公开(公告)号：US20130157332A1
公开(公告)日：2013-06-20
申请号：US13711647
申请日：2012-12-12
发明人： WILLIAM D. HITZ , Min Qi , Luan Tao , Paul V. Viitanen , Jianjun Yang
IPC分类号： C12N15/74
CPC分类号： C12N15/74 , C12N9/0006 , C12N9/1022 , C12N9/1205 , C12N9/92 , C12P7/065 , C12P7/10 , C12Y101/01021 , C12Y202/01001 , C12Y202/01002 , C12Y207/01017 , Y02E50/16 , Y02E50/17
摘要： Zymomonas cells that are genetically engineered to have a disrupted aldose reductase gene such that aldose reductase activity for conversion of xylose to xylitol in the presence of NADPH is reduced by greater than 90%, and that are engineered to express a xylose utilization metabolic pathway, were found to have the ability to gro on medium containing xylose as the only sugar without adaptation in media containing xylose.
摘要翻译： 遗传工程化以具有破坏的醛糖还原酶基因的酵母单胞菌细胞，使得在NADPH存在下将木糖转化为木糖醇的醛糖还原酶活性降低大于90％，并且被工程化以表达木糖利用代谢途径 发现具有在含有木糖的培养基中含有木糖的能力，作为唯一的糖，而不含有含木糖的培养基。
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公开(公告)号：US20140178954A1
公开(公告)日：2014-06-26
申请号：US13792668
申请日：2013-03-11
发明人： WILLIAM D HITZ , Min Qi , Sarah Eve Rush , Luan Tao , Paul V. Viitanen , Jianjun Yang , Rick W. Ye
IPC分类号： C12N15/81
摘要： Expression of a xylose isomerase in a yeast cell that expresses the chaperonins GroES and GroEL was found to result in enzymatically active xylose isomerase, while there is little to no activity with expression of the bacterial xylose isomerase in a yeast cell lacking GroES and GroEL. A yeast cell expressing xylose isomerase activity, and a complete xylose utilization pathway, provides a yeast cell that can produce a target compound, such as ethanol, butanol, or 1,3-propanediol, using xylose derived from lignocellulosic biomass as a carbon source.
摘要翻译： 发现表达伴侣蛋白GroES和GroEL的酵母细胞中木糖异构酶的表达导致酶活性木糖异构酶，而在缺乏GroES和GroEL的酵母细胞中，细菌木糖异构酶的表达几乎没有活性。 表达木糖异构酶活性的酵母细胞和完全的木糖利用途径提供了可以使用衍生自木质纤维素生物质的木糖作为碳源产生目标化合物如乙醇，丁醇或1,3-丙二醇的酵母细胞。
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4.发明申请PNP GENE MODIFICATION FOR IMPROVED XYLOSE UTILIZATION IN ZYMOMONAS 有权用于改善ZYMOMONAS中的XYLOSE利用的PNP基因修饰公开(公告)号：US20130157331A1
公开(公告)日：2013-06-20
申请号：US13711646
申请日：2012-12-12
发明人： PERRY G. CAIMI , Min Qi , Luan Tao , Paul V. Viitanen , Jianjun Yang
IPC分类号： C12N15/74
CPC分类号： C12N15/74 , C12N9/1258 , C12N9/90 , C12N9/92 , C12P7/065 , C12P7/10 , C12Y207/07008 , C12Y501/03001 , C12Y503/01005 , C12Y503/01006 , Y02E50/16 , Y02E50/17
摘要： The endogenous pnp gene encoding polynucleotide phosphorylase in the Zymomonas genome was identified as a target for modification to provide improved xylose utilizing cells for ethanol production. The cells are in addition genetically modified to have increased expression of ribose-5-phosphate isomerase (RPI) activity, as compared to cells without this genetic modification, and are not limited in xylose isomerase activity in the absence of the pnp modification.
摘要翻译： 在酵母单位基因组中编码多核苷酸磷酸化酶的内源性pnp基因被鉴定为用于修饰以提供用于乙醇生产的细胞的改良木糖的靶标。 与没有这种遗传修饰的细胞相比，细胞另外进行遗传修饰以具有增加的核糖-5-磷酸异构酶（RPI）活性的表达，并且在没有pnp修饰的情况下不限制于木糖异构酶活性。
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