公开(公告)号：US09278138B2

公开(公告)日：2016-03-08

申请号：US14191871

申请日：2014-02-27

申请人： E. I. DU PONT DE NEMOURS AND COMPANY

发明人： Qiong Cheng ,  Stephen R. Fahnestock ,  Hongxian He ,  Kristy N. Kostichka ,  Hong Wang

IPC分类号： C07K7/04 ,  C07K7/08 ,  A61K38/04 ,  A61K38/16 ,  A61Q5/00 ,  A61K47/48 ,  C07K14/00 ,  G01N33/68 ,  G06F19/16 ,  A61K8/64

CPC分类号： A61K47/48338 ,  A61K8/19 ,  A61K8/64 ,  A61K2800/94 ,  A61Q5/00 ,  A61Q5/065 ,  C07K14/001 ,  C07K2319/70 ,  G01N33/6803 ,  G06F19/16

摘要： Short single chain peptides having affinity for a target surface often lack the binding durability required for certain commercial applications. One way to improve durability is to promote multivalent binding by linking together binding sequences using peptide linkers. However, the resulting single chain binding peptides often suffer from linker entropy. It has been discovered that the use of rigid peptide linkers when linking together multiple binding sequences enhances the binding affinity of the resulting single chain peptide.

摘要翻译： 对目标表面具有亲和性的短单链肽通常缺乏某些商业应用所需的结合耐久性。 提高耐久性的一种方法是通过使用肽接头连接在一起的结合序列来促进多价结合。 然而，所得到的单链结合肽通常具有接头熵。 已经发现，当将多个结合序列连接在一起时，使用刚性肽接头增强所得单链肽的结合亲和力。

公开(公告)号：US20140241999A1

公开(公告)日：2014-08-28

申请号：US14191871

申请日：2014-02-27

申请人： E. I. DU PONT DE NEMOURS AND COMPANY

发明人： Qiong Cheng ,  Stephen R. Fahnestock ,  Hongxian He ,  Kristy N. Kostichka ,  Hong Wang

IPC分类号： A61K47/48 ,  A61Q5/00 ,  A61K8/64 ,  C07K14/00

CPC分类号： A61K47/48338 ,  A61K8/19 ,  A61K8/64 ,  A61K2800/94 ,  A61Q5/00 ,  A61Q5/065 ,  C07K14/001 ,  C07K2319/70 ,  G01N33/6803 ,  G06F19/16

摘要： Short single chain peptides having affinity for a target surface often lack the binding durability required for certain commercial applications. One way to improve durability is to promote multivalent binding by linking together binding sequences using peptide linkers. However, the resulting single chain binding peptides often suffer from linker entropy. It has been discovered that the use of rigid peptide linkers when linking together multiple binding sequences enhances the binding affinity of the resulting single chain peptide.

摘要翻译： 对目标表面具有亲和性的短单链肽通常缺乏某些商业应用所需的结合耐久性。 提高耐久性的一种方法是通过使用肽接头连接在一起的结合序列来促进多价结合。 然而，所得到的单链结合肽通常具有接头熵。 已经发现，当将多个结合序列连接在一起时，使用刚性肽接头增强所得单链肽的结合亲和力。

公开(公告)号：US20140080998A1

公开(公告)日：2014-03-20

申请号：US14077342

申请日：2013-11-12

申请人： E I DU PONT DE NEMOURS AND COMPANY

发明人： LAURA A BEDZYK ,  Stephen R. Fahnestock ,  Tanja Maria Gruber ,  Daniel P. O'Keefe ,  Pierre E Rouviere

IPC分类号： C12P21/06

CPC分类号： C07K5/1021 ,  C07K1/12 ,  C07K7/06 ,  C07K14/435 ,  C07K2319/00 ,  C07K2319/01 ,  C07K2319/50 ,  C12N15/62 ,  C12P21/06

摘要： An acid-cleavable peptide linker comprising aspartic acid and proline residues is disclosed. The acid-cleavable peptide linker provides an altered sensitivity to acid-hydrolytic release of peptides of interest from fusion peptides of the formula PEP1-L-PEP2. The inventive linker, L, is described in various embodiments, each of which provides substantially more rapid acid-release of peptides of interest than does a single aspartic acid-proline pair. In an additional aspect, a method of increasing the stability of an acid cleavable linkage to acid hydrolysis is also provided.

公开(公告)号：US20140221608A1

公开(公告)日：2014-08-07

申请号：US14252928

申请日：2014-04-15

申请人： E I DU PONT DE NEMOURS AND COMPANY

发明人： LAURA A BEDZYK ,  Stephen R. Fahnestock ,  Tanja Maria Gruber ,  Daniel P. O'Keefe ,  Pierre E Rouviere

IPC分类号： C07K5/113 ,  C07K7/06 ,  C07K14/435 ,  C12P21/06

CPC分类号： C07K5/1021 ,  C07K1/12 ,  C07K7/06 ,  C07K14/435 ,  C07K2319/00 ,  C07K2319/01 ,  C07K2319/50 ,  C12N15/62 ,  C12P21/06

摘要： An acid-cleavable peptide linker comprising aspartic acid and proline residues is disclosed. The acid-cleavable peptide linker provides an altered sensitivity to acid-hydrolytic release of peptides of interest from fusion peptides of the formula PEP1-L-PEP2. The inventive linker, L, is described in various embodiments, each of which provides substantially more rapid acid-release of peptides of interest than does a single aspartic acid-proline pair. In an additional aspect, a method of increasing the stability of an acid cleavable linkage to acid hydrolysis is also provided.

摘要翻译： 公开了包含天冬氨酸和脯氨酸残基的可酸切割的肽接头。 酸可切割肽接头提供对式PEP1-L-PEP2的融合肽对感兴趣的肽的酸水解释放的改变的敏感性。 在各种实施方案中描述了本发明的接头L，其各自提供比单一天冬氨酸 - 脯氨酸对显着更快速地释放目的肽。 在另一方面，还提供了提高酸可切割键与酸水解的稳定性的方法。

公开(公告)号：US20140073029A1

公开(公告)日：2014-03-13

申请号：US14078772

申请日：2013-11-13

申请人： E I DU PONT DE NEMOURS AND COMPANY

发明人： ROBERT DICOSIMO ,  Scott D. Cunningham ,  Stephen R. Fahnestock ,  Tanja Maria Gruber ,  Mark S. Payne ,  Pierre E. Rouviere ,  Linda Jane Solomon ,  Hong Wang

IPC分类号： C12N9/18 ,  C12N9/42 ,  C07K16/16

CPC分类号： C12N9/18 ,  C07K16/16 ,  C07K19/00 ,  C07K2319/01 ,  C11D3/38636 ,  C11D3/38681 ,  C12N9/14 ,  C12N9/2437 ,  C12P7/40 ,  C12P7/54

摘要： Disclosed herein are compositions and methods to target enzymatic peracid production to a target surface. The peracid benefit agent produced by the targeted perhydrolytic enzyme can be use for a variety of applications such as bleaching, whitening, disinfecting, destaining, deodorizing, and combinations thereof. Specifically, a fusion protein comprising a perhydrolytic enzyme and at least one peptidic component having affinity for a target surface (excluding body surfaces and oral care surfaces) is used in combination with a suitable substrate and a source of peroxygen to enzymatically produce a peracid on or near the surface of the target material. In a preferred aspect, the target surface is a cellulosic material.

摘要翻译： 本文公开了将酶过氧酸生产靶向靶表面的组合物和方法。 由靶向的水解酶产生的过酸有益剂可用于各种应用，例如漂白，增白，消毒，脱色，除臭及其组合。 特别地，包含过水解酶和至少一种对靶表面（不包括身体表面和口腔护理表面）具有亲和性的肽成分的融合蛋白与合适的底物和过氧源组合使用以在其上或 靠近目标材料的表面。 在优选的方面，目标表面是纤维素材料。