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公开(公告)号：US09295947B2
公开(公告)日：2016-03-29
申请号：US14190560
申请日：2014-02-26
Applicant: EMD Millipore Corporation
Inventor： Louis Bonhomme , John Doyle , Kurt Greenizen , George Gagne , Sean Devin , Gerard Lombard , Vincent Smith
CPC classification number: B01D63/16 , B01D61/145 , B01D61/18 , B01D63/08 , B01D2313/08 , B01L3/502 , B01L3/5021 , B01L3/5635 , B01L2300/0681
Abstract: Filtration device suited for concentration of liquid samples, particularly biomolecules, and a method of concentrating, desalting, purifying and/or fractionating liquid samples. In certain embodiments the device includes a housing having a sample reservoir, and two substantially vertically oriented and spaced apart membranes disposed in the housing. An underdrain is associated with each membrane such that fluid passing through each membrane flows through a respective underdrain into a filtrate collection chamber. The fluid that does not pass through the membrane is collected in the retentate collection chamber, and can be recovered such as by a reverse spinning step, achieving recoveries greater than about 90%. The substantially vertical orientation of the membranes increases the available membrane area by at least 2.7 times the area available in a conventional filter device. The two-panel configuration also maintains more available membrane area in use during the last stages of filtration than a one-panel configuration.
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公开(公告)号：US20140224723A1
公开(公告)日：2014-08-14
申请号：US14190560
申请日：2014-02-26
Applicant: EMD Millipore Corporation
Inventor： Louis Bonhomme , John Doyle , Kurt Greenizen , George Gagne , Sean Devin , Gerard Lombard , Vincent Smith
CPC classification number: B01D63/16 , B01D61/145 , B01D61/18 , B01D63/08 , B01D2313/08 , B01L3/502 , B01L3/5021 , B01L3/5635 , B01L2300/0681
Abstract: Filtration device suited for concentration of liquid samples, particularly biomolecules, and a method of concentrating, desalting, purifying and/or fractionating liquid samples. In certain embodiments the device includes a housing having a sample reservoir, and two substantially vertically oriented and spaced apart membranes disposed in the housing. An underdrain is associated with each membrane such that fluid passing through each membrane flows through a respective underdrain into a filtrate collection chamber. The fluid that does not pass through the membrane is collected in the retentate collection chamber, and can be recovered such as by a reverse spinning step, achieving recoveries greater than about 90%. The substantially vertical orientation of the membranes increases the available membrane area by at least 2.7 times the area available in a conventional filter device. The two-panel configuration also maintains more available membrane area in use during the last stages of filtration than a one-panel configuration.
Abstract translation: 适用于浓缩液体样品，特别是生物分子的过滤装置，以及浓缩，脱盐，纯化和/或分馏液体样品的方法。 在某些实施方案中，该装置包括具有样品储存器的壳体和设置在壳体中的两个基本垂直定向和间隔开的膜。 与每个膜相关联的下划线，使得通过每个膜的流体通过相应的下划线流入滤液收集室。 不通过膜的流体被收集在滞留物收集室中，并且可以通过反转纺步骤回收，实现大于约90％的回收率。 膜的基本垂直取向将可用的膜面积增加了传统过滤装置中可用面积的至少2.7倍。 两面板配置在过滤的最后阶段比在单面板配置中还维持使用更多的可用膜面积。
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公开(公告)号：US08747670B2
公开(公告)日：2014-06-10
申请号：US13649365
申请日：2012-10-11
Applicant: EMD Millipore Corporation
Inventor： Louis Bonhomme , John Doyle , Kurt Greenizen , George Gagne , Sean Devin , Gerard Lombard , Vincent Smith
CPC classification number: B01D63/16 , B01D61/145 , B01D61/18 , B01D63/08 , B01D2313/08 , B01L3/502 , B01L3/5021 , B01L3/5635 , B01L2300/0681
Abstract: Filtration device suited for concentration of liquid samples, particularly biomolecules, and a method of concentrating, desalting, purifying and/or fractionating liquid samples. In certain embodiments the device includes a housing having a sample reservoir, and two substantially vertically oriented and spaced apart membranes disposed in the housing. An underdrain is associated with each membrane such that fluid passing through each membrane flows through a respective underdrain into a filtrate collection chamber. The fluid that does not pass through the membrane is collected in the retentate collection chamber, and can be recovered such as by a reverse spinning step, achieving recoveries greater than about 90%. The substantially vertical orientation of the membranes increases the available membrane area by at least 2.7 times the area available in a conventional filter device. The two-panel configuration also maintains more available membrane area in use during the last stages of filtration than a one-panel configuration.
Abstract translation: 适用于浓缩液体样品，特别是生物分子的过滤装置，以及浓缩，脱盐，纯化和/或分馏液体样品的方法。 在某些实施方案中，该装置包括具有样品储存器的壳体和设置在壳体中的两个基本垂直定向和间隔开的膜。 与每个膜相关联的下划线，使得通过每个膜的流体通过相应的下划线流入滤液收集室。 不通过膜的流体被收集在滞留物收集室中，并且可以通过反转纺步骤回收，实现大于约90％的回收率。 膜的基本垂直取向将可用的膜面积增加了传统过滤装置中可用面积的至少2.7倍。 两面板配置在过滤的最后阶段比在单面板配置中还维持使用更多的可用膜面积。
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公开(公告)号：US09897520B2
公开(公告)日：2018-02-20
申请号：US14974334
申请日：2015-12-18
Applicant: EMD Millipore Corporation
Inventor： Christopher A. Scott , Timothy K. Nadler , Kurt Greenizen , Louis Bonhomme , Sara D. Gutierrez , Masaharu Mabuchi , David Briggs , Phillip Clark , Ralph T. Scaduto
CPC classification number: G01N1/34 , B01D61/18 , B01D63/06 , B01D63/16 , B01D2315/02 , B01L3/5021 , B01L3/5085 , B01L2300/0618 , B01L2300/0681 , C07K1/16 , C07K1/22 , G01N1/405 , G01N1/4077 , G01N2001/4088 , Y10T436/25375
Abstract: Sample preparation device that allows for a complete bind, wash, elute, buffer-exchange and concentration process to be carried out without sample transfer between multiple devices. The device includes a reservoir, a column for holding chromatography media, a holder region for holding a filtration device, and an outlet. The filtration device plugs into the holder region of the centrifugal device, and the assembly can be placed in an optional holder. The assembly, with or without the optional holder, can be placed in a conventional centrifuge tube for centrifugation. The entire bind, wash, elute, buffer exchange and concentration steps can be carried out with the apparatus without any pipette transfers (and the associated sample losses. The sample preparation device also can be used for binding and washing steps, in which case the filtration device is not needed, and for buffer exchange and concentration steps, in which case the media is not needed.
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Patent Agency Ranking
公开(公告)号：US20140017151A1
公开(公告)日：2014-01-16
申请号：US14031458
申请日：2013-09-19
Applicant: EMD Millipore Corporation
Inventor： Christopher A. Scott , Timothy K. Nadler , Kurt Greenizen , Louis Bonhomme , Sara D. Gutierrez , Masaharu Mabuchi , David Briggs , Phillip Clark , Ralph T. Scaduto
IPC: G01N1/34
CPC classification number: G01N1/34 , B01D61/18 , B01D63/06 , B01D63/16 , B01D2315/02 , B01L3/5021 , B01L3/5085 , B01L2300/0618 , B01L2300/0681 , C07K1/16 , C07K1/22 , G01N1/405 , G01N1/4077 , G01N2001/4088 , Y10T436/25375
Abstract: Sample preparation device that allows for a complete bind, wash, elute, buffer-exchange and concentration process to be carried out without sample transfer between multiple devices. The device includes a reservoir, a column for holding chromatography media, a holder region for holding a filtration device, and an outlet. The filtration device plugs into the holder region of the centrifugal device, and the assembly can be placed in an optional holder. The assembly, with or without the optional holder, can be placed in a conventional centrifuge tube for centrifugation. The entire bind, wash, elute, buffer exchange and concentration steps can be carried out with the apparatus without any pipette transfers (and the associated sample losses. The sample preparation device also can be used for binding and washing steps, in which case the filtration device is not needed, and for buffer exchange and concentration steps, in which case the media is not needed.
Abstract translation: 允许完全结合，洗涤，洗脱，缓冲交换和浓缩过程的样品制备装置，在多个装置之间没有样品转移的情况下进行。 该装置包括储存器，用于保持色谱介质的柱，用于保持过滤装置的保持器区域和出口。 过滤装置插入到离心装置的保持器区域中，并且组件可以放置在可选的保持器中。 具有或不具有任选的保持器的组件可以放置在用于离心的常规离心管中。 整个结合，洗涤，洗脱，缓冲液交换和浓缩步骤可以在没有任何移液管转移（和相关样品损失）的情况下进行，样品制备装置也可用于结合和洗涤步骤，在这种情况下，过滤 设备不需要，并且用于缓冲区交换和集中步骤，在这种情况下不需要介质。
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公开(公告)号：US20160103045A1
公开(公告)日：2016-04-14
申请号：US14974334
申请日：2015-12-18
Applicant: EMD Millipore Corporation
Inventor： Christopher A. Scott , Timothy K. Nadler , Kurt Greenizen , Louis Bonhomme , Sara D. Gutierrez , Masaharu Mabuchi , David Briggs , Phillip Clark , Ralph T. Scaduto
CPC classification number: G01N1/34 , B01D61/18 , B01D63/06 , B01D63/16 , B01D2315/02 , B01L3/5021 , B01L3/5085 , B01L2300/0618 , B01L2300/0681 , C07K1/16 , C07K1/22 , G01N1/405 , G01N1/4077 , G01N2001/4088 , Y10T436/25375
Abstract: Sample preparation device that allows for a complete bind, wash, elute, buffer-exchange and concentration process to be carried out without sample transfer between multiple devices. The device includes a reservoir, a column for holding chromatography media, a holder region for holding a filtration device, and an outlet. The filtration device plugs into the holder region of the centrifugal device, and the assembly can be placed in an optional holder. The assembly, with or without the optional holder, can be placed in a conventional centrifuge tube for centrifugation. The entire bind, wash, elute, buffer exchange and concentration steps can be carried out with the apparatus without any pipette transfers (and the associated sample losses. The sample preparation device also can be used for binding and washing steps, in which case the filtration device is not needed, and for buffer exchange and concentration steps, in which case the media is not needed.
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公开(公告)号：US09103756B2
公开(公告)日：2015-08-11
申请号：US14031458
申请日：2013-09-19
Applicant: EMD Millipore Corporation
Inventor： Christopher A. Scott , Timothy K. Nadler , Kurt Greenizen , Louis Bonhomme , Sara D. Gutierrez , Masaharu Mabuchi , David Briggs , Phillip Clark , Ralph T. Scaduto
CPC classification number: G01N1/34 , B01D61/18 , B01D63/06 , B01D63/16 , B01D2315/02 , B01L3/5021 , B01L3/5085 , B01L2300/0618 , B01L2300/0681 , C07K1/16 , C07K1/22 , G01N1/405 , G01N1/4077 , G01N2001/4088 , Y10T436/25375
Abstract: Sample preparation device that allows for a complete bind, wash, elute, buffer-exchange and concentration process to be carried out without sample transfer between multiple devices. The device includes a reservoir, a column for holding chromatography media, a holder region for holding a filtration device, and an outlet. The filtration device plugs into the holder region of the centrifugal device, and the assembly can be placed in an optional holder. The assembly, with or without the optional holder, can be placed in a conventional centrifuge tube for centrifugation. The entire bind, wash, elute, buffer exchange and concentration steps can be carried out with the apparatus without any pipette transfers (and the associated sample losses. The sample preparation device also can be used for binding and washing steps, in which case the filtration device is not needed, and for buffer exchange and concentration steps, in which case the media is not needed.
Abstract translation: 允许完全结合，洗涤，洗脱，缓冲交换和浓缩过程的样品制备装置，在多个装置之间没有样品转移的情况下进行。 该装置包括储存器，用于保持色谱介质的柱，用于保持过滤装置的保持器区域和出口。 过滤装置插入到离心装置的保持器区域中，并且组件可以放置在可选的保持器中。 具有或不具有任选的保持器的组件可以放置在用于离心的常规离心管中。 整个结合，洗涤，洗脱，缓冲液交换和浓缩步骤可以在没有任何移液管转移（和相关样品损失）的情况下进行，样品制备装置也可用于结合和洗涤步骤，在这种情况下，过滤 设备不需要，并且用于缓冲区交换和集中步骤，在这种情况下不需要介质。
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公开(公告)号：US09050565B2
公开(公告)日：2015-06-09
申请号：US13739008
申请日：2013-01-11
Applicant: EMD Millipore Corporation
Inventor： Louis Bonhomme , John Doyle , Kurt Greenizen , George Gagne , Sean Devin , Gerard Lombard , Vincent Smith
CPC classification number: B01D63/16 , B01D61/145 , B01D61/18 , B01D63/08 , B01D2313/08 , B01L3/502 , B01L3/5021 , B01L3/5635 , B01L2300/0681
Abstract: Filtration device suited for concentration of liquid samples, particularly biomolecules, and a method of concentrating, desalting, purifying and/or fractionating liquid samples. In certain embodiments the device includes a housing having a sample reservoir, and two substantially vertically oriented and spaced apart membranes disposed in the housing. An underdrain is associated with each membrane such that fluid passing through each membrane flows through a respective underdrain into a filtrate collection chamber. The fluid that does not pass through the membrane is collected in the retentate collection chamber, and can be recovered such as by a reverse spinning step, achieving recoveries greater than about 90%. The substantially vertical orientation of the membranes increases the available membrane area by at least 2.7 times the area available in a conventional filter device. The two-panel configuration also maintains more available membrane area in use during the last stages of filtration than a one-panel configuration.
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公开(公告)号：US20130186830A1
公开(公告)日：2013-07-25
申请号：US13739008
申请日：2013-01-11
Applicant: EMD Millipore Corporation
Inventor： Louis Bonhomme , John Doyle , Kurt Greenizen , George Gagne , Sean Devin , Gerard Lombard , Vincent Smith
IPC: B01D63/16
CPC classification number: B01D63/16 , B01D61/145 , B01D61/18 , B01D63/08 , B01D2313/08 , B01L3/502 , B01L3/5021 , B01L3/5635 , B01L2300/0681
Abstract: Filtration device suited for concentration of liquid samples, particularly biomolecules, and a method of concentrating, desalting, purifying and/or fractionating liquid samples. In certain embodiments the device includes a housing having a sample reservoir, and two substantially vertically oriented and spaced apart membranes disposed in the housing. An underdrain is associated with each membrane such that fluid passing through each membrane flows through a respective underdrain into a filtrate collection chamber. The fluid that does not pass through the membrane is collected in the retentate collection chamber, and can be recovered such as by a reverse spinning step, achieving recoveries greater than about 90%. The substantially vertical orientation of the membranes increases the available membrane area by at least 2.7 times the area available in a conventional filter device. The two-panel configuration also maintains more available membrane area in use during the last stages of filtration than a one-panel configuration.
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