公开(公告)号：US20240124822A1

公开(公告)日：2024-04-18

申请号：US17953889

申请日：2022-09-27

申请人： EMULATE, Inc.

发明人： Christopher David Hinojosa ,  Josiah Sliz ,  Iosif Pediaditakis ,  Sonalee Barthakur

IPC分类号： C12M1/00 ,  B01L3/00 ,  C12M3/06

CPC分类号： C12M29/10 ,  B01L3/502738 ,  C12M23/16 ,  C12M29/14 ,  B01L2300/0861 ,  B01L2400/0605 ,  G01N1/18

摘要： The invention generally relates to a microfluidic platforms or “chips” for testing and conducting experiments on the International Space Station (ISS). More specifically, microfluidic Brain-On-Chip, comprising neuronal and vascular endothelial cells, will be analyzed in both healthy and inflamed states to assess how the circumstances of space travel affect the human brain.

公开(公告)号：US11697792B2

公开(公告)日：2023-07-11

申请号：US16712439

申请日：2019-12-12

申请人： EMULATE, INC.

发明人： Christopher David Hinojosa ,  Josiah Sliz ,  Iosif Pediaditakis ,  Sonalee Barthakur

IPC分类号： C12M1/00 ,  B01L3/00 ,  C12M3/06 ,  G01N1/18 ,  C12M1/26

CPC分类号： C12M29/10 ,  B01L3/502738 ,  C12M23/16 ,  C12M29/14 ,  B01L2300/0861 ,  B01L2400/0605 ,  C12M33/00 ,  G01N1/18

摘要： The invention generally relates to a microfluidic platforms or “chips” for testing and conducting experiments on the International Space Station (ISS). More specifically, microfluidic Brain-On-Chip, comprising neuronal and vascular endothelial cells, will be analyzed in both healthy and inflamed states to assess how the circumstances of space travel affect the human brain.

公开(公告)号：US12098356B2

公开(公告)日：2024-09-24

申请号：US17953889

申请日：2022-09-27

申请人： EMULATE, Inc.

发明人： Christopher David Hinojosa ,  Josiah Sliz ,  Iosif Pediaditakis ,  Sonalee Barthakur

IPC分类号： C12M1/00 ,  B01L3/00 ,  C12M3/06 ,  C12M1/26 ,  G01N1/18

CPC分类号： C12M29/10 ,  B01L3/502738 ,  C12M23/16 ,  C12M29/14 ,  B01L2300/0861 ,  B01L2400/0605 ,  C12M33/00 ,  G01N1/18

摘要： The invention generally relates to a microfluidic platforms or “chips” for testing and conducting experiments on the International Space Station (ISS). More specifically, microfluidic Brain-On-Chip, comprising neuronal and vascular endothelial cells, will be analyzed in both healthy and inflamed states to assess how the circumstances of space travel affect the human brain.

公开(公告)号：US20220340849A1

公开(公告)日：2022-10-27

申请号：US17719627

申请日：2022-04-13

申请人： EMULATE, INC.

发明人： Iosif Pediaditakis ,  William R. Tien-Street ,  S. Jordan Kerns ,  Geraldine Hamilton ,  Daniel Levner

IPC分类号： C12M3/06 ,  C12N5/079 ,  C12N5/00 ,  C07K14/47 ,  C12M1/12 ,  G01N33/50

摘要： The invention relates to modeling brain neuronal disease in a microfluidic device, comprising a co-culture of iPS-derived brain endothelial cells; iPS-derived dopaminergic neurons; primary microglia; and primary astrocytes, a Blood-Brain-Barrier (BBB)-Chip and a Brain-Chip. In particular, cross-talk between glial cells (e.g. microglia and astrocytes) with neuronal cells, in further contact with endothelial cells is contemplated for use for identifying drug targets under conditions for inducing in vivo relevant neuronal inflammation, neurodegeneration and neuronal death. Thus, in one embodiment, a microfluidic Brain-Chip comprising a co-culture of brain cells is exposed to α-synuclein preformed fibrils (PFF), a type of pathogenic form of α-synuclein. Such α-synuclein PFF exposure demonstrates an in vivo relevant disease pathogenesis on a microfluidic device as a concentration- and time-controlled manner that may be used for preclinical drug evaluation for diseases related to neuronal inflammation, e.g. Parkinson's disease (PD). In some embodiments, modulation of complement in the presence of neuronal inflammation is contemplated. In some embodiments, drug delivery to brain cells across the BBB is contemplated for preclinical testing of drug efficacy for slowing or stopping neuronal inflammation and degeneration.