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    Transiently immortalized cells for use in gene therapy
    1.
    发明授权
    Transiently immortalized cells for use in gene therapy 有权
    用于基因治疗的瞬时永生细胞

    公开(公告)号：US06451601B1

    公开(公告)日：2002-09-17

    申请号：US09823177

    申请日：2001-03-29

    申请人： Edward E. Baetge ,  Shou Wong ,  Philippe Dupraz ,  Bernard Thorens

    发明人： Edward E. Baetge ,  Shou Wong ,  Philippe Dupraz ,  Bernard Thorens

    IPC分类号： C12N508

    CPC分类号： C12N9/1276 ,  C07K2319/10

    摘要： The invention provides methods and compositions for expanding cells that are not abundant or are difficult to obtain in pure form in culture, are in short supply (e.g., human cells), or have brief lifetimes in culture, using fusion polypeptide. The fusion polypeptide has a first region containing a translocation carrier moiety having the function of a transport polypeptide amino acid sequence from, e.g., herpesviral VP22, HIV TAT, Antp HD, Arg repeats, or a cationic polymer, or from homologues or fragments thereof, and a second region with a polypeptide having cell immortalization activity, a polypeptide having telomerase-specific activity, or a polypeptide having telomerase gene activation activity. The resulting cells of the invention are suitable for use in cell therapy.

    摘要翻译： 本发明提供用于扩增细胞的方法和组合物，所述细胞在培养物中不是丰富的或难以获得纯的形式，在短缺（例如人细胞）中或使用融合多肽在培养中具有短暂的寿命。 融合多肽具有包含具有来自例如疱疹病毒VP22，HIV TAT，Antp HD，Arg重复序列或阳离子聚合物或其同源物或片段的转运多肽氨基酸序列的功能的易位载体部分的第一区域， 和具有细胞永生化活性的多肽的第二区域，具有端粒酶特异性活性的多肽或具有端粒酶基因激活活性的多肽。 本发明的所得细胞适合用于细胞治疗。

    Transiently immortalized cells
    2.
    发明授权
    Transiently immortalized cells 有权
    瞬时永生细胞

    公开(公告)号：US06358739B1

    公开(公告)日：2002-03-19

    申请号：US09546483

    申请日：2000-04-10

    申请人： Edward E. Baetge ,  Shou Wong ,  Philippe Dupraz ,  Bernard Thorens

    发明人： Edward E. Baetge ,  Shou Wong ,  Philippe Dupraz ,  Bernard Thorens

    IPC分类号： C12N508

    CPC分类号： C07K14/82 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/10 ,  C12N9/1276 ,  C12N2710/16622 ,  C12N2740/16045 ,  C12N2740/16322

    摘要： The invention provides methods and compositions for expanding cells that are not abundant or are difficult to obtain in pure form in culture, are in short supply (e.g., human cells), or have brief lifetimes in culture, using fusion polypeptide. The fusion polypeptide has a first region having the transport function of herpesviral VP22 protein or human immunodeficiency virus (HIV) TAT protein, and a second region with a polypeptide having cell immortalization activity, a polypeptide having telomerase-specific activity, or a polypeptide having telomerase gene activation activity. The resulting cells of the invention are suitable for use in cell therapy.

    摘要翻译： 本发明提供用于扩增细胞的方法和组合物，所述细胞在培养物中不是丰富的或难以获得纯的形式，在短缺（例如人细胞）中或使用融合多肽在培养中具有短暂的寿命。 融合多肽具有疱疹病毒VP22蛋白或人类免疫缺陷病毒（HIV）TAT蛋白的转运功能的第一区域和具有细胞永生化活性的多肽的第二区域，具有端粒酶活性的多肽或具有端粒酶的多肽 基因激活活性。 本发明的所得细胞适合用于细胞治疗。

    Compositions for the delivery of biologically active molecules using genetically altered cells contained in biocompatible immunoisolatory capsules
    7.
    发明授权
    Compositions for the delivery of biologically active molecules using genetically altered cells contained in biocompatible immunoisolatory capsules 失效
    使用包含在生物相容性免疫隔离胶囊中的遗传改变细胞递送生物活性分子的组合物

    公开(公告)号：US06264941B1

    公开(公告)日：2001-07-24

    申请号：US09236246

    申请日：1999-01-25

    申请人： Edward E. Baetge ,  Joseph P. Hammang ,  Frank T. Gentile ,  Mark D. Lindner ,  Shelley R. Winn ,  Dwaine F. Emerich

    发明人： Edward E. Baetge ,  Joseph P. Hammang ,  Frank T. Gentile ,  Mark D. Lindner ,  Shelley R. Winn ,  Dwaine F. Emerich

    IPC分类号： A61K4800

    CPC分类号： A61K9/0092 ,  A61K9/0024 ,  A61K9/0085 ,  A61K38/00 ,  A61K48/00 ,  C07K14/48 ,  C07K14/70 ,  C12N15/87

    摘要： This invention provides improved devices and methods for long-term, stable expression of a biologically active molecule using a biocompatible capsule containing genetically engineered cells for the effective delivery of biologically active molecules to effect or enhance a biological function within a mammalian host. The novel capsules of this invention are biocompatible and are easily retrievable. This invention specifically provides improved methods and compositions which utilize cells transfected with recombinant DNA molecules comprising DNA sequences coding for biologically active molecules operatively linked to promoters that are not subject to down regulation in vivo upon implantation into a mammalian host. Furthermore, the methods of this invention allow for the long-term, stable and efficacious delivery of biologically active molecules from living cells to specific sites within a given mammal. In addition, this invention provides a general means for maintaining, for extended periods of time, the in vivo expression of transgenes.

    摘要翻译： 本发明提供改进的装置和方法，用于使用包含基因工程细胞的生物相容性胶囊长期稳定地表达生物活性分子，用于有效递送生物活性分子以实现或增强哺乳动物宿主内的生物学功能。 本发明的新颖胶囊是生物相容的并且易于回收。 本发明具体提供改进的方法和组合物，其利用重组DNA分子转染的细胞，所述重组DNA分子包含编码与植入哺乳动物宿主体内不受体内下调的启动子有效连接的生物活性分子的DNA序列。 此外，本发明的方法允许将生物活性分子从活细胞长期，稳定和有效地递送到给定哺乳动物内的特定位点。 此外，本发明提供了用于长时间维持转基因的体内表达的一般方法。