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公开(公告)号：US6051556A
公开(公告)日：2000-04-18
申请号：US438465
申请日：1995-05-10
IPC分类号： C07K14/325 , C07K14/34 , C12N15/62 , A61K38/00 , C07K1/00
CPC分类号： C12N15/62 , C07K14/325 , C07K14/34 , C07K2319/00 , C07K2319/035 , C07K2319/55
摘要： The invention concerns novel hybrid pesticidal toxins. These toxins are expressed as the fusion protein of a chimeric gene. Specifically exemplified is a novel B.t. hybrid toxin. These novel toxins have increased toxicity against target pests. The invention also concerns a process for preparing a hybrid virus having an altered insect host range.
摘要翻译： 本发明涉及新型杂种杀虫毒素。 这些毒素被表达为嵌合基因的融合蛋白。 具体例子是一个小说B.t. 杂交毒素。 这些新型毒素对目标害虫的毒性增加。 本发明还涉及制备具有改变的昆虫宿主范围的杂种病毒的方法。
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2.发明授权Process for altering the host range or increasing the toxicity of Bacillus thuringiensis lepidoteran toxins, and recombinant DNA sequences therefor 失效改变宿主范围或增加苏云金芽胞杆菌鳞翅目活性毒素的毒性的方法及其重组DNA序列
公开(公告)号：US6090931A
公开(公告)日：2000-07-18
申请号：US855160
申请日：1997-05-13
IPC分类号： C12N15/09 , A01N63/00 , A01N63/02 , C07K14/00 , C07K14/325 , C07K14/41 , C12N1/00 , C12N1/21 , C12N15/00 , C12N15/32 , C12P21/02 , C12R1/07 , C12R1/19 , C12R1/38 , C12N15/62 , C12N15/70 , C12N15/82
CPC分类号： C12R1/075 , A01N63/00 , A01N63/02 , C07K14/325
摘要： The invention concerns an in vitro process for altering the insect host range (spectrum) or increasing the toxicity of lepidopteran active B.t. crystal protein toxins. The process comprises recombining in vitro the variable region(s) (non-homologous) of two or more genes encoding lepidopteran active B.t. crystal protein toxins. Specifically exemplified is the recombining of the variable regions of two genes obtained from well-known strains of Bacillus thuringiensis var. kurstaki. The resulting products are chimeric toxins which are shown to have an expanded and/or amplified lepidopteran insect host range as compared to the parent toxins.
摘要翻译： 本发明涉及用于改变昆虫宿主范围（光谱）或增加鳞翅目活性B.t.的毒性的体外方法。 晶体蛋白毒素。 该方法包括在体外重组编码鳞翅目昆虫活性B.t.的两个或多个基因的可变区（非同源的）。 晶体蛋白毒素。 具体举例说明了从已知的苏云金芽孢杆菌变种菌株获得的两个基因的可变区的重组。 kurstaki。 所得产物是与母体毒素相比显示具有扩增和/或扩增的鳞翅目昆虫宿主范围的嵌合毒素。
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3.发明授权Hybrid Bacillus thuringiensis gene, plasmid and transformed Pseudomonas fluorescens 失效混合苏云金芽孢杆菌基因，质粒和转化的荧光假单胞菌
公开(公告)号：US5128130A
公开(公告)日：1992-07-07
申请号：US568650
申请日：1990-08-14
申请人： Thomas E. Gilroy , Edward R. Wilcox
发明人： Thomas E. Gilroy , Edward R. Wilcox
IPC分类号： A61K38/00 , C07K14/325 , C12N1/21 , C12N15/32 , C12P1/04
CPC分类号： C07K14/325 , C12P1/04 , A61K38/00 , Y10S435/832 , Y10S435/848 , Y10S435/874
摘要： A novel hybrid B.t. toxin gene toxic to lepidopteran insects has been cloned. The DNA encoding the B.t. toxin can be used to transform various prokaryotic and eukaryotic microbes to express the B.t. toxin. These recombinant microbes can be used to control lepidopteran insects in various environments.
摘要翻译： 一种新型杂种 已经克隆了对鳞翅目昆虫有毒的毒素基因。 编码B.t.的DNA 毒素可用于转化各种原核和真核微生物，以表达B.t. 毒素。 这些重组微生物可用于控制各种环境中的鳞翅目昆虫。
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4.发明授权Method, vectors, and host cells for the control of expression of heterologous genes from lac operated promoters 失效用于控制来自LAC操作的促进剂的异源基因表达的方法，载体和宿主细胞
公开(公告)号：US5169760A
公开(公告)日：1992-12-08
申请号：US386821
申请日：1989-07-27
申请人： Edward R. Wilcox
发明人： Edward R. Wilcox
CPC分类号： C12N15/72
摘要： The invention is a process for controlling the expression of heterologous genes from lac-operated promoters by removing the CAP binding site and lac promoter from the lac operon. Illustrated is the fusion of the lacZ, Y, and A genes of the lac operon to the 3' end of the lacI structural gene. This elimination of the natural regulatory elements of the lac operon results, advantageously, in the production of lac operon gene products in a constitutive mode from the lacI promoter.
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