公开(公告)号：US20090028880A1

公开(公告)日：2009-01-29

申请号：US11587749

申请日：2006-05-17

申请人： Els Beirnaert ,  Hilde Adi Pierrette Revets ,  Hendricus Renerus Hoogenboom

发明人： Els Beirnaert ,  Hilde Adi Pierrette Revets ,  Hendricus Renerus Hoogenboom

IPC分类号： A61K39/395 ,  C07K16/18 ,  C07H21/04 ,  A61P7/00 ,  C12N5/10 ,  C12P21/00

CPC分类号： C07K16/241 ,  A61K2039/505 ,  C07K14/525 ,  C07K16/18 ,  C07K16/468 ,  C07K2317/22 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/565 ,  C07K2319/31

摘要： The present invention relates to amino acid sequences that are capable of binding to serum albumin, which sequences do not significantly reduce or inhibit the binding of serum albumin to FcRn or significantly reduce the half-life of serum albumin. It further relates to proteins and polypeptides comprising or essentially consisting of such amino acid sequences; to nucleic acids that encode such amino acid sequences, proteins or polypeptides; to compositions, and in particular pharmaceutical compositions, that comprise such amino acid sequences, proteins and polypeptides; and to uses of such amino acid sequences, proteins and polypeptides.

摘要翻译： 本发明涉及能够结合血清白蛋白的氨基酸序列，该序列不显着降低或抑制血清白蛋白与FcRn的结合或显着降低血清白蛋白的半衰期。 它还涉及包含或基本上由这样的氨基酸序列组成的蛋白质和多肽; 涉及编码这种氨基酸序列，蛋白质或多肽的核酸; 包括这样的氨基酸序列，蛋白质和多肽的组合物，特别是药物组合物; 以及这些氨基酸序列，蛋白质和多肽的使用。

公开(公告)号：US20070232790A1

公开(公告)日：2007-10-04

申请号：US11625769

申请日：2007-01-22

申请人： ANDREW GRIFFITHS ,  Hendricus Renerus Hoogenboom ,  James Marks ,  John McCafferty ,  Gregory Winter ,  Geoffrey Grigg

发明人： ANDREW GRIFFITHS ,  Hendricus Renerus Hoogenboom ,  James Marks ,  John McCafferty ,  Gregory Winter ,  Geoffrey Grigg

IPC分类号： C07K16/18

CPC分类号： C40B40/02 ,  B01J2219/00718 ,  C07K16/005 ,  C07K16/18 ,  C07K16/22 ,  C07K16/241 ,  C07K16/26 ,  C07K16/28 ,  C07K16/2812 ,  C07K16/2863 ,  C07K16/2866 ,  C07K16/3007 ,  C07K16/3092 ,  C07K16/34 ,  C07K16/40 ,  C07K16/4208 ,  C07K16/4241 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/62 ,  Y10S530/867

摘要： Methods are disclosed for the production of anti-self antibodies and antibody fragments, being antibodies or fragments of a particular species of mammal which bind self antigens of that species. Methods comprise providing a library of replicable genetic display packages (rgdps), such as filamentous phage, each rgdp displaying at its surface member of a specific binding pair which is an antibody or antibody fragment, and each rgdp containing nucleic acid sequence derived from a species of mammal. The nucleic acid sequence in each rgdp encodes a polypeptide chain which is a component part of the sbp member displayed at the surface of that rgdp. Anti-self antibody fragments are selected by binding with a self antigen from said species of mammal. The displayed antibody fragments may be scFv, Fd, Fab or any other fragment which has the capability of binding antigen. Nucleic acid libraries used may be derived from rearranged V-gene sequences of unimmunised mammal. Synthetic or artificial libraries are described and shown to be useful.

摘要翻译： 公开了用于产生抗自身抗体和抗体片段的方法，其是结合该物种的自身抗原的特定哺乳动物种类的抗体或片段。 方法包括提供可复制的遗传显示包（rgdps）文库，例如丝状噬菌体，每个rgdp在其作为抗体或抗体片段的特异性结合对的表面成员显示，并且每个含有rgdp的核酸序列衍生自物种 的哺乳动物。 每个rgdp中的核酸序列编码多肽链，其是在该rgdp的表面显示的sbp成员的组成部分。 通过与来自所述哺乳动物物种的自身抗原结合来选择抗自身抗体片段。 显示的抗体片段可以是scFv，Fd，Fab或具有结合抗原能力的任何其它片段。 所用的核酸文库可能来源于未免疫哺乳动物的重排的V基因序列。 描述和显示合成或人造文库是有用的。

公开(公告)号：US20090155810A1

公开(公告)日：2009-06-18

申请号：US11555464

申请日：2006-11-01

申请人： John McCafferty ,  Anthony Richard Pope ,  Kevin Stuart Johnson ,  Hendricus Renerus Hoogenboom ,  Andrew David Griffiths ,  Ronald Henry Jackson ,  Kasper Philipp Holliger ,  James David Marks ,  Timothy Piers Clackson ,  David John Chiswell ,  Gregory Paul Winter ,  Timothy Peter Bonnert

发明人： John McCafferty ,  Anthony Richard Pope ,  Kevin Stuart Johnson ,  Hendricus Renerus Hoogenboom ,  Andrew David Griffiths ,  Ronald Henry Jackson ,  Kasper Philipp Holliger ,  James David Marks ,  Timothy Piers Clackson ,  David John Chiswell ,  Gregory Paul Winter ,  Timothy Peter Bonnert

IPC分类号： G01N33/53 ,  C12N15/63

CPC分类号： C07K16/005 ,  B01J2219/00718 ,  C07K16/00 ,  C07K16/18 ,  C07K16/26 ,  C07K16/34 ,  C07K16/40 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K19/00 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/62 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/62 ,  C12N15/73 ,  C40B40/02 ,  G01N33/53 ,  G01N33/6857 ,  Y10S530/867

摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.

摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。

公开(公告)号：US09738701B2

公开(公告)日：2017-08-22

申请号：US12931955

申请日：2011-02-14

申请人： Hendricus Renerus Hoogenboom ,  Ton Logtenberg

发明人： Hendricus Renerus Hoogenboom ,  Ton Logtenberg

IPC分类号： C07K16/10 ,  C07K16/22 ,  C07K16/00

CPC分类号： G01N33/56966 ,  C07K16/005 ,  C07K16/10 ,  C07K16/22 ,  C07K16/248 ,  C07K16/2863 ,  C07K16/32 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/34 ,  C07K2317/52 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2317/94 ,  C07K2319/00

摘要： The present invention provides combinations of specific binding proteins, such as immunoglobulins, that are designed to be true combinations, essentially all components of the combination being functional and compatible with each other. The invention further provides a method for producing a composition comprising at least two different proteinaceous molecules comprising paired variable regions, the at least two proteinaceous molecules having different binding specificities, comprising paired variable regions, at least two proteinaceous molecules having different binding specificities, comprising contacting at least three different variable regions under conditions allowing for pairing of variable regions and harvesting essentially all proteinaceous molecules having binding specificities resulting from the pairing.

公开(公告)号：US20070059766A1

公开(公告)日：2007-03-15

申请号：US11490545

申请日：2006-07-20

申请人： Ton Logtenberg ,  Hendricus Renerus Hoogenboom

发明人： Ton Logtenberg ,  Hendricus Renerus Hoogenboom

IPC分类号： C40B30/06 ,  C40B40/02 ,  C40B50/06

CPC分类号： C12N15/1086 ,  C07K16/10 ,  C07K16/20 ,  C07K16/40 ,  C07K2317/22 ,  C07K2317/569 ,  C07K2318/20 ,  C12N15/1082

摘要： Described are methods for producing libraries of cells expressing at least two separate single polypeptide chain binding proteins, in which the binding proteins have different target epitopes. Such libraries are made by integration of the nucleic acid sequences encoding the polypeptide chains into the genome of the host cell, and selecting for cells that have successfully integrated these nucleic acids. The selected cells are preferably subjected to a cloning step. Mixtures of binding proteins are produced without having to individually produce each of the components of the mixture. A library of cells wherein essentially each cell encodes at least two single polypeptide chain binding proteins having different target epitopes is also herewith provided, as well as methods for producing a composition comprising at least two separate single polypeptide chain binding proteins having different target epitopes.

摘要翻译： 描述了用于产生表达至少两个单独的多肽链结合蛋白的细胞文库的方法，其中结合蛋白具有不同的靶表位。 通过将编码多肽链的核酸序列整合到宿主细胞的基因组中并选择成功整合这些核酸的细胞来制备此类文库。 所选择的细胞优选进行克隆步骤。 产生结合蛋白的混合物，而不必单独产生混合物的每种组分。 还提供了其中基本上每个细胞编码至少两种具有不同靶表位的单个多肽链结合蛋白的细胞库，以及用于产生包含至少两种具有不同靶表位的单独多肽链结合蛋白的组合物的方法。