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    Process for Culturing Adherent Cells
    1.
    发明申请
    Process for Culturing Adherent Cells 有权
    培养粘附细胞的方法

    公开(公告)号：US20110151506A1

    公开(公告)日：2011-06-23

    申请号：US12976679

    申请日：2010-12-22

    申请人： Eric Calvosa ,  Nicolas Seve

    发明人： Eric Calvosa ,  Nicolas Seve

    IPC分类号： C12P1/00 ,  C12N5/10

    CPC分类号： C12N5/00 ,  C12N7/00 ,  C12N2500/92 ,  C12N2500/99 ,  C12N2760/20151

    摘要： The subject of the present invention is a process for producing adherent cells comprising: a. introducing a suspension of adherent cells into a culture vessel containing microcarriers in a culture medium; b. amplifying the cells by performing a plurality of cell passages in the same culture vessel wherein each cell passage subsequent to the first is carried out: i) by using all or part of the cells produced during the previous cell passage after having subjected the cells to enzyme treatment to detach the cells from the microcarriers, and ii) by introducing culture medium and an increasing amount of microcarriers into the culture medium; and c. harvesting the cells produced during the last cell passage, optionally after having subjected the cells to enzyme treatment to detach cells from microcarriers. The invention also relates to the implementation of this process for the production of biological agents, serving in particular to prepare vaccines or drugs.

    摘要翻译： 本发明的主题是制备贴壁细胞的方法，包括：a。 将粘附细胞的悬浮液引入培养基中含有微载体的培养容器中; b。 通过在相同的培养容器中进行多个细胞通道来扩增细胞，其中进行第一次之后的每个细胞通道：i）通过在使细胞进入酶之后使用在先前细胞传代期间产生的全部或部分细胞 处理以从微载体中分离细胞，和ii）通过将培养基和增加量的微载体导入培养基中; 和c。 收集在最后一个细胞传代期间产生的细胞，任选地在使细胞进行酶处理以从微载体分离细胞之后。 本发明还涉及用于生产生物制剂的该方法的实施，特别是用于制备疫苗或药物。

    Process for culturing adherent cells
    2.
    发明授权
    Process for culturing adherent cells 有权
    培养贴壁细胞的方法

    公开(公告)号：US09145543B2

    公开(公告)日：2015-09-29

    申请号：US12976679

    申请日：2010-12-22

    申请人： Eric Calvosa ,  Nicolas Seve

    发明人： Eric Calvosa ,  Nicolas Seve

    IPC分类号： C12P1/00 ,  C12N1/00 ,  C12N5/00 ,  C12N7/00

    CPC分类号： C12N5/00 ,  C12N7/00 ,  C12N2500/92 ,  C12N2500/99 ,  C12N2760/20151

    摘要： The subject of the present invention is a process for producing adherent cells comprising: a. introducing a suspension of adherent cells into a culture vessel containing microcarriers in a culture medium; b. amplifying the cells by performing a plurality of cell passages in the same culture vessel wherein each cell passage subsequent to the first is carried out: i) by using all or part of the cells produced during the previous cell passage after having subjected the cells to enzyme treatment to detach the cells from the microcarriers, and ii) by introducing culture medium and an increasing amount of microcarriers into the culture medium; and c. harvesting the cells produced during the last cell passage, optionally after having subjected the cells to enzyme treatment to detach cells from microcarriers. The invention also relates to the implementation of this process for the production of biological agents, serving in particular to prepare vaccines or drugs.

    摘要翻译： 本发明的主题是制备贴壁细胞的方法，包括：a。 将粘附细胞的悬浮液引入培养基中含微载体的培养容器中; b。 通过在相同的培养容器中进行多个细胞通道来扩增细胞，其中进行第一次之后的每个细胞通道：i）通过在使细胞进入酶之后使用在先前细胞传代期间产生的全部或部分细胞 处理以从微载体中分离细胞，和ii）通过将培养基和增加量的微载体导入培养基中; 和c。 收集在最后一个细胞传代期间产生的细胞，任选地在使细胞进行酶处理以从微载体分离细胞之后。 本发明还涉及用于生产生物制剂的该方法的实施，特别是用于制备疫苗或药物。

    Method for purifying the rabies virus
    5.
    发明申请
    Method for purifying the rabies virus 有权
    纯化狂犬病病毒的方法

    公开(公告)号：US20100260798A1

    公开(公告)日：2010-10-14

    申请号：US12756590

    申请日：2010-04-08

    申请人： Virginie Fabre ,  Céline Rocca ,  Pierre Riffard ,  Eric Calvosa

    发明人： Virginie Fabre ,  Céline Rocca ,  Pierre Riffard ,  Eric Calvosa

    IPC分类号： A61K39/205 ,  A61P31/14 ,  C12N7/02 ,  C12N7/06

    CPC分类号： A61K39/205 ,  A61K39/12 ,  C12N7/00 ,  C12N2760/20134 ,  C12N2760/20151

    摘要： The subject of the invention is a method for purifying the rabies virus, comprising a single ion-exchange chromatography step, said step being cation exchange chromatography according to which: a) the supernatant of a culture of cells infected with this virus is brought into contact with a cation exchange chromatography support comprising a polymethacrylate matrix onto which sulfoisobutyl groups have been grafted such that the rabies virus binds to this support, and; b) the virus is eluted from its support.

    摘要翻译： 本发明的目的是一种纯化狂犬病病毒的方法，包括单个离子交换层析步骤，所述步骤为阳离子交换色谱法，其中：a）将感染该病毒的细胞培养物的上清液接触 使用包含聚甲基丙烯酸酯基质的阳离子交换层析支持物，其上已经接枝有磺基异丁基，使得狂犬病毒结合该载体， b）病毒从其支持物上洗脱出来。

    Method for Purifying the Rabies Virus
    6.
    发明申请
    Method for Purifying the Rabies Virus 有权
    纯化狂犬病病毒的方法

    公开(公告)号：US20150216963A1

    公开(公告)日：2015-08-06

    申请号：US14593337

    申请日：2015-01-09

    申请人： Virginie Fabre ,  Celine Rocca ,  Pierre Riffard ,  Eric Calvosa

    发明人： Virginie Fabre ,  Celine Rocca ,  Pierre Riffard ,  Eric Calvosa

    IPC分类号： A61K39/205 ,  C12N7/00

    CPC分类号： A61K39/205 ,  A61K39/12 ,  C12N7/00 ,  C12N2760/20134 ,  C12N2760/20151

    摘要： The subject of the invention is a method for purifying the rabies virus, comprising a single ion-exchange chromatography step, said step being cation exchange chromatography according to which: a) the supernatant of a culture of cells infected with this virus is brought into contact with a cation exchange chromatography support comprising a polymethacrylate matrix onto which sulfoisobutyl groups have been grafted such that the rabies virus binds to this support, and; b) the virus is eluted from its support.

    摘要翻译： 本发明的目的是一种纯化狂犬病病毒的方法，包括单个离子交换层析步骤，所述步骤为阳离子交换层析，其中：a）将感染该病毒的细胞培养物的上清液接触 使用包含聚甲基丙烯酸酯基质的阳离子交换层析支持物，其上已经接枝有磺基异丁基，使得狂犬病毒结合该载体， b）病毒从其支持物上洗脱出来。

    Method for purifying the rabies virus
    7.
    发明授权
    Method for purifying the rabies virus 有权
    纯化狂犬病病毒的方法

    公开(公告)号：US08961997B2

    公开(公告)日：2015-02-24

    申请号：US12756590

    申请日：2010-04-08

    申请人： Virginie Fabre ,  Céline Rocca ,  Pierre Riffard ,  Eric Calvosa

    发明人： Virginie Fabre ,  Céline Rocca ,  Pierre Riffard ,  Eric Calvosa

    IPC分类号： B01D15/08 ,  B01D15/00 ,  C02F1/42 ,  A61K39/205 ,  C12N7/00 ,  A61K39/12

    CPC分类号： A61K39/205 ,  A61K39/12 ,  C12N7/00 ,  C12N2760/20134 ,  C12N2760/20151

    摘要： The subject of the invention is a method for purifying the rabies virus, comprising a single ion-exchange chromatography step, said step being cation exchange chromatography according to which: a) the supernatant of a culture of cells infected with this virus is brought into contact with a cation exchange chromatography support comprising a polymethacrylate matrix onto which sulfoisobutyl groups have been grafted such that the rabies virus binds to this support, and; b) the virus is eluted from its support.

    摘要翻译： 本发明的目的是一种纯化狂犬病病毒的方法，包括单个离子交换层析步骤，所述步骤为阳离子交换层析，其中：a）将感染该病毒的细胞培养物的上清液接触 使用包含聚甲基丙烯酸酯基质的阳离子交换层析支持物，其上已经接枝有磺基异丁基，使得狂犬病毒结合该载体， b）病毒从其支持物上洗脱出来。