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公开(公告)号：US06525029B1
公开(公告)日：2003-02-25
申请号：US09182567
申请日：1998-10-29
IPC分类号： A61K4800
CPC分类号： C12N15/86 , A61K38/00 , A61K48/00 , C07K14/70517 , C07K14/70578 , C07K14/7151 , C07K14/7156 , C07K2319/00 , C12N2710/10343
摘要： The present invention provides, among other things, a method of inhibiting an immune response to a recombinant vector, such as a viral vector, specifically an adenoviral vector. The method comprises contacting a cell with (i) a recombinant vector, preferably a viral vector, most preferably an adenoviral vector, comprising a transgene and (ii) a means of inhibiting an immune response to the recombinant vector selected from the group consisting of a TNF receptor fusion protein, a Fas receptor fusion protein, an IFN receptor fusion protein, a gene encoding a TNF receptor fusion protein, a gene encoding a Fas receptor fusion protein, and a gene encoding an IFN receptor fusion protein, whereupon an immune response to the recombinant vector is inhibited. In this regard, the present invention also provides a recombinant vector and a composition for use in the method.
摘要翻译： 本发明尤其提供了抑制重组载体如病毒载体，特别是腺病毒载体的免疫应答的方法。 该方法包括使细胞与（i）包含转基因的重组载体，优选病毒载体，最优选腺病毒载体接触，和（ii）抑制对重组载体的免疫应答的方法，所述重组载体选自 TNF受体融合蛋白，Fas受体融合蛋白，IFN受体融合蛋白，编码TNF受体融合蛋白的基因，编码Fas受体融合蛋白的基因和编码IFN受体融合蛋白的基因， 重组载体被抑制。 在这方面，本发明还提供了用于该方法的重组载体和组合物。
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公开(公告)号：US07261885B2
公开(公告)日：2007-08-28
申请号：US10911957
申请日：2004-08-05
IPC分类号： A61K48/00 , C12N15/861
CPC分类号： C12N15/86 , A61K48/00 , C12N2710/10343 , C12N2840/44
摘要： The invention provides an adenoviral vector comprising (a) at least a portion of an adenoviral genome comprising a major late transcription unit containing a terminal exon, wherein the terminal exon comprises a 5′ splice acceptor DNA sequence element and a 3′ polyadenylation signal sequence, and (b) a non-native nucleic acid sequence encoding a protein that does not contribute to the adenoviral vector entry into a host cell, wherein the non-native nucleic acid sequence is positioned within the terminal exon, such that the non-native nucleic acid sequence is selectively expressed in cells within which the adenoviral vector can replicate. The invention further provides an adenoviral vector composition and a method for treating or preventing a pathologic state in a mammal, comprising administering to the mammal the adenoviral vector composition of the invention in an amount sufficient to treat or prevent the pathologic state in the mammal.
摘要翻译： 本发明提供了一种腺病毒载体，其包含（a）至少一部分腺病毒基因组，其包含含有末端外显子的主要晚期转录单位，其中所述末端外显子包含5'剪接受体DNA序列元件和3'多腺苷酸化信号序列， 和（b）编码不对腺病毒载体进入宿主细胞有贡献的蛋白质的非天然核酸序列，其中非天然核酸序列位于末端外显子内，使得非天然核酸 酸序列在腺病毒载体可以复制的细胞中选择性表达。 本发明进一步提供了一种用于治疗或预防哺乳动物病理状态的腺病毒载体组合物和方法，其包括以足以治疗或预防哺乳动物病理状态的量向哺乳动物施用本发明的腺病毒载体组合物。
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公开(公告)号：US5849561A
公开(公告)日：1998-12-15
申请号：US861773
申请日：1997-05-22
IPC分类号： C12N15/09 , A61K48/00 , C12N7/01 , C12N15/861
CPC分类号： C12N15/86 , C12N2710/10343 , C12N2830/002
摘要： A method of producing a replication deficient adenovirus comprising a passenger gene and a deficiency in an essential gene function of the E1 region of an adenovirus comprising producing the adenovirus in a cell that provides in trans gene functions of the E1 and E4 regions of one or more adenoviruses not belonging to the same serogroup as the replication deficient adenovirus.
摘要翻译： 一种生产复制缺陷型腺病毒的方法，包括乘客基因和腺病毒E1区域的必需基因功能缺陷，包括在提供一个或多个的E1和E4区域的转基因功能的细胞中产生腺病毒 不属于与复制缺陷型腺病毒相同血清群的腺病毒。
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4.发明申请USE OF AAV INTEGRATION EFFICIENCY ELEMENT FOR MEDIATING SITE-SPECIFIC INTEGRATION OF A TRANSCRIPTION UNIT 审中-公开使用AAV整合效率元素介绍转录单位的网站特定整合公开(公告)号：US20110166207A1
公开(公告)日：2011-07-07
申请号：US12546288
申请日：2009-08-24
IPC分类号： A61K31/7088 , C12N15/74 , A61P31/12
CPC分类号： C12N15/86 , A61K48/00 , C07K14/005 , C12N15/90 , C12N2710/10343 , C12N2710/10344 , C12N2750/14122 , C12N2750/14143 , C12N2800/30
摘要： The invention provides an expression construct comprising a nucleic acid sequence encoding an adeno-associated virus integration efficiency element (AAV IEE), wherein the expression construct is substantially devoid of AAV inverted terminal repeats (AAV ITRs). Such an expression construct site-specifically integrates into a host cell chromosome when provided to a host cell in conjunction with an AAV Rep protein. The invention also provides a method of integrating a nucleic acid sequence of interest into a host cell chromosome through use of such an expression construct, as well as a method of prophylactically or therapeutically treating a mammal for a pathologic state comprising administering to a mammal such an expression construct comprising a nucleic acid sequence encoding a therapeutic factor.
摘要翻译： 本发明提供了包含编码腺相关病毒整合效率元件（AAV IEE）的核酸序列的表达构建体，其中所述表达构建体基本上没有AAV反向末端重复序列（AAV ITR）。 当与AAV Rep蛋白结合提供给宿主细胞时，这种表达构建位点特异性地整合到宿主细胞染色体中。 本发明还提供了通过使用这种表达构建体将感兴趣的核酸序列整合到宿主细胞染色体中的方法，以及预防或治疗哺乳动物治疗病理状态的方法，包括向哺乳动物施用 表达构建体，其包含编码治疗因子的核酸序列。
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公开(公告)号：US5837511A
公开(公告)日：1998-11-17
申请号：US537402
申请日：1995-10-02
CPC分类号： C12N15/86 , A61K48/00 , C12N2710/10343
摘要： The present invention provides replication-deficient non-group C adenoviral vectors. Also provided is a therapeutic method, particularly relating to gene therapy, vaccination, and the like, involving the use of such vectors incorporating a foreign nucleic acid.
摘要翻译： 本发明提供了复制缺陷型非C组腺病毒载体。 还提供了一种特别涉及基因治疗，疫苗接种等的治疗方法，涉及使用包含外来核酸的这种载体。
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公开(公告)号：US06824770B1
公开(公告)日：2004-11-30
申请号：US08653114
申请日：1996-05-24
IPC分类号： A01N6300
CPC分类号： C12N15/86 , C07K14/57 , C07K14/723 , C12N2710/10343 , C12N2830/38 , C12N2840/44
摘要： The invention is directed to an adenoviral vector comprising (a) at least one insertion site for cloning a heterologous gene, and, in an orientation opposite to the direction of transcription of the adenoviral region into which it is inserted, (b) a heterologous promoter positioned upstream from the insertion site, (c) a eukaryotic splice acceptor and splice donor site positioned between the promoter and the insertion site; and (c) a polyadenylation sequence positioned downstream of the insertion site. The invention also provides a host cell infected with such a vector, a method of producing a selected protein, and a method of delivering a heterologous gene to an animal heart.
摘要翻译： 本发明涉及一种腺病毒载体，其包含（a）用于克隆异源基因的至少一个插入位点，并且与其插入的腺病毒区域的转录方向相反的方向，（b）异源启动子 位于插入位点的上游，（c）位于启动子和插入位点之间的真核接头受体和剪接供体位点; 和（c）位于插入位点下游的多聚腺苷酸化序列。 本发明还提供了用这种载体感染的宿主细胞，产生选择的蛋白质的方法，以及将异源基因递送到动物心脏的方法。
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公开(公告)号：US06599737B1
公开(公告)日：2003-07-29
申请号：US09699314
申请日：2000-10-30
IPC分类号： C12N15861
CPC分类号： C07K14/005 , A61K48/00 , C12N15/86 , C12N2710/10322 , C12N2710/10343 , C12N2710/10345 , C12N2810/6018
摘要： The present invention provides an adenoviral gene transfer vector comprising a first fiber gene and a second fiber gene, wherein the fiber genes are different. The present invention also provides related recombinant adenoviral gene transfer vectors and methods of propagating an adenovirus with a fiber protein that does not bind to a native adenoviral fiber receptor.
摘要翻译： 本发明提供了包含第一纤维基因和第二纤维基因的腺病毒基因转移载体，其中纤维基因不同。 本发明还提供了相关的重组腺病毒基因转移载体和用不与天然腺病毒纤维受体结合的纤维蛋白扩增腺病毒的方法。
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